Diachasmimorpha longicaudata Parasitism Response to Medfly Host Fruit and Fruit Infestation Age

Harbi, Ahlem; Pedro, Luis de; Ferrara, Fernando; Tormos, José; Chermiti, Brahim; Beitia, Francisco J.; Sabater-Muñoz, Beatriz

doi:10.3390/insects10070211

Cited by 17 publications

(26 citation statements)

References 57 publications

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“…Harbi and colleagues demonstrated a multistep assay (e.g., olfactory, laboratory, and semi-field trials) and reported the preference of medfly-infected fruits, including apple, orange, peach, and clementine mandarins [18]. This experiment supports that different olfactory genes are expressed in response to different fruit scents.…”

Section: Introductionmentioning

confidence: 89%

De Novo Transcriptome Identifies Olfactory Genes in Diachasmimorpha longicaudata (Ashmead)

Tang

Liu

et al. 2020

Genes

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Diachasmimoorpha longicaudata (Ashmead, D. longicaudata) (Hymenoptera: Braconidae) is a solitary species of parasitoid wasp and widely used in integrated pest management (IPM) programs as a biological control agent in order to suppress tephritid fruit flies of economic importance. Although many studies have investigated the behaviors in the detection of their hosts, little is known of the molecular information of their chemosensory system. We assembled the first transcriptome of D. longgicaudata using transcriptome sequencing and identified 162,621 unigenes for the Ashmead insects in response to fruit flies fed with different fruits (guava, mango, and carambola). We annotated these transcripts on both the gene and protein levels by aligning them to databases (e.g., NR, NT, KEGG, GO, PFAM, UniProt/SwissProt) and prediction software (e.g., SignalP, RNAMMER, TMHMM Sever). CPC2 and MIREAP were used to predict the potential noncoding RNAs and microRNAs, respectively. Based on these annotations, we found 43, 69, 60, 689, 26 and 14 transcripts encoding odorant-binding protein (OBP), chemosensory proteins (CSPs), gustatory receptor (GR), odorant receptor (OR), odorant ionotropic receptor (IR), and sensory neuron membrane protein (SNMP), respectively. Sequence analysis identified the conserved six Cys in OBP sequences and phylogenetic analysis further supported the identification of OBPs and CSPs. Furthermore, 9 OBPs, 13 CSPs, 3 GRs, 4IRs, 25 ORs, and 4 SNMPs were differentially expressed in the insects in response to fruit flies with different scents. These results support that the olfactory genes of the parasitoid wasps were specifically expressed in response to their hosts with different scents. Our findings improve our understanding of the behaviors of insects in the detection of their hosts on the molecular level. More importantly, it provides a valuable resource for D. longicaudata research and will benefit the IPM programs and other researchers in this filed.

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Section: Introductionmentioning

confidence: 89%

De Novo Transcriptome Identifies Olfactory Genes in Diachasmimorpha longicaudata (Ashmead)

Tang

Liu

et al. 2020

Genes

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“…Aganaspis pelleranoi has been reported to be more attracted to volatiles of guavas infested with frugivorous larvae [ 47 ]. For its part, the females of D. longicaudata , which is one of the most widely employed species against tephritids [ 13 ], have also shown a significant preference for fruits infested with larvae of the medfly and/or other fruit flies [ 36 , 48 ]. In this regard, larvae in rotting fruits have long been believed to release or induce the formation of volatiles that are attractive to parasitoids when compared with uninfested ripe fruit [ 49 , 50 , 51 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

“…This was connected to an air pump producing a unidirectional airflow of 150 mL/min from the arms to the base (wind speed of 0.005 m/s). Airflow was established based on previous experiments with similar-sized insects [ 30 , 34 , 35 , 36 ]. The air pump was connected to two 5-L crystal jars containing the odor sources to be evaluated (in this case, test fruits).…”

Section: Methodsmentioning

confidence: 99%

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A Minor Role of Host Fruit on the Parasitic Performance of Aganaspis daci (Hymenoptera: Figitidae) on Medfly Larvae

Pedro

Harbi

Tormos

et al. 2021

Insects

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Host fruit is known to strongly affect the performance of both fruit pests and their potential natural enemies. This is particularly important in the control of tephritid fruit flies, whose larvae develop inside the fruit and thus create a set of foraging problems for parasitoids. In the present study, we assessed the response of female Aganaspis daci (Weld) (Hymenoptera: Figitidae), one of the most promising parasitoids for tephritid biocontrol in the Mediterranean Basin, to different potential host fruit species. We measured the olfactory response to medfly-infested and uninfested fruits, and several biological parameters of A. daci when different infested fruits were offered under both laboratory and greenhouse conditions. Our results showed that this parasitoid was significantly more attracted to apples and uninfested fruit. Moreover, parasitic activity was similar among the tested fruits under both conditions, showing very high values in the laboratory and a much poorer performance when conditions were variable. This suggests that A. daci may be a good candidate to be included in mass releases against the medfly regardless of the affected crop, but only when climate conditions are not expected to hinder its normal activity.

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“…Direct and indirect damage is caused by Anastrepha and Ceratitis, thus the biological control of fruit flies has great economic and environmental importance, providing increased research aimed at the use of the organisms Diachasmimorpha Research, Society and Development, v. 10, n. 1, e22510111245, 2021 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i1.11245 longicaudata, Doryctobracon areolatus, Beauveria bassiana, Wolbachia, and Bacillus thuringiensis, who are responsible for controlling this pest (Harbi et al, 2019).…”

Section: Control Agents Analyzed By Categoriesmentioning

confidence: 99%

Biological control of fruit flies: bibliometric analysis on the main biocontrol agents

Salustino

Celedônio

Filho

et al. 2021

RSD

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The Tephritidae family has many fruit fly species responsible for causing direct and indirect damage to economically important fruit trees worldwide. Biological control has been sought as a method for the management of these insects, mainly because it does not cause adverse damage to the environment. Thus, this review sought information on what is currently being published in the scientific field about the main biological agents that are used to control fruit flies. The information was obtained through surveys between the months of June and August 2020, in bases such as the Web of Science, Scopus, ScienceDirect, Taylor & Francis, Springer, and Scielo. The inclusion of the articles followed criteria such as publication language English, Portuguese and Spanish, available in full, from categories A1 to B1, related to the biological agents used in the control of fruit flies and published in the last five years. A total of 2,362 studies were found, of which 105 articles were selected for this review. Regarding the years of publication, only 27% of the studies correspond to references from the years 2019 and 2020, with a greater number of research on parasitoids and developed in the laboratory. The largest concentration of research was in countries like Brazil, Mexico, and Spain.

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Diachasmimorpha longicaudata Parasitism Response to Medfly Host Fruit and Fruit Infestation Age

Cited by 17 publications

References 57 publications

De Novo Transcriptome Identifies Olfactory Genes in Diachasmimorpha longicaudata (Ashmead)

De Novo Transcriptome Identifies Olfactory Genes in Diachasmimorpha longicaudata (Ashmead)

A Minor Role of Host Fruit on the Parasitic Performance of Aganaspis daci (Hymenoptera: Figitidae) on Medfly Larvae

Biological control of fruit flies: bibliometric analysis on the main biocontrol agents

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