Diacylglycerol Activates the Light-Dependent Channel TRP in the Photosensitive Microvilli of<i>Drosophila melanogaster</i>Photoreceptors

Delgado, Ricardo; Muñoz, Yorka; Peña‐Cortés, Hugo; Giavalisco, Patrick; Bacigalupo, Juan

doi:10.1523/jneurosci.0513-14.2014

Cited by 44 publications

(39 citation statements)

References 36 publications

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“…Proposed candidates include IP3 (Brown et al, 1984;Fein et al, 1984) or Ca 2+ (Payne et al, 1986) in Limulus polyphemus; DAG or its metabolites, e.g. PUFAs (Chyb et al, 1999;del Pilar Gomez and Nasi, 1998;Delgado et al, 2014) in Drosophila or scallop; protons (Huang et al, 2010) and mechanical forces (Hardie and Franze, 2012) in Drosophila. The next step of the cascade, namely the role of TRP channels in DO and JCs photoconductance has been confirmed and appears similar to that in invertebrate photoreceptors and ipRGCs.…”

Section: Rhabdomeric Photoreceptorsmentioning

confidence: 99%

Amphioxus photoreceptors - insights into the evolution of vertebrate opsins, vision and circadian rhythmicity

Pergner

Kozmík²

2017

Int. J. Dev. Biol.

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Studies on amphioxus, representing the most basal group of chordates, can give insights into the evolution of vertebrate traits. The present review of amphioxus research is focused on the physiology of light-guided behavior as well as on the fine structure, molecular biology, and electrophysiology of the nervous system, with special attention being given to the photoreceptive organs. The amphioxus visual system is especially interesting because four types of receptors are involved in light detection -dorsal ocelli and Joseph cells (both rhabdomeric photoreceptors) and the frontal eye and lamellar body (both ciliary photoreceptors). Here, we consider how the available information on photoreceptive organs and light-guided behavior in amphioxus helps generate hypotheses about the history of these features during chordate and subsequently vertebrate evolution.

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Section: Rhabdomeric Photoreceptorsmentioning

confidence: 99%

Amphioxus photoreceptors - insights into the evolution of vertebrate opsins, vision and circadian rhythmicity

Pergner

Kozmík²

2017

Int. J. Dev. Biol.

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“…Thus far we have not encountered analytical methods that were incompatible with the obtained fractions and in most cases the obtained abundance and the quality of the compounds from the MTBE-derived extracts was reaching sensitivities and quality to the more specialized extraction methods. Beyond the optimal applicability of our method for plant cells and tissues, it should be mentioned that we have not only applied this method thus far for diverse plant samples [23, 27, 32, 48–54], but it was also successfully employed for metabolic and/or proteomic studies of algae [55, 56], flies [57] and diverse mammalian cells and tissues [58–60]. …”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the method allowed the detection and annotation of more than 180 lipid species from Chlamydomonas reinhardtii [55]. Furthermore, we applied the described method to determine the lipid composition of the green algal species Scenedesmus (Acutodesmus) obliquus [56] and the model fly Drosophila melanogaster [57]. Moreover, the method has been proven useful also for lipid profiling of 124 lipid species from the marine diatom ( Thalassiosira pseudonana ) [53, 61] and the biddulphioid diatom ( Biddulphia biddulphiana ) [61].…”

Section: Discussionmentioning

confidence: 99%

Protocol: a fast, comprehensive and reproducible one-step extraction method for the rapid preparation of polar and semi-polar metabolites, lipids, proteins, starch and cell wall polymers from a single sample

et al. 2016

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BackgroundThe elucidation of complex biological systems requires integration of multiple molecular parameters. Accordingly, high throughput methods like transcriptomics, proteomics, metabolomics and lipidomics have emerged to provide the tools for successful system-wide investigations. Unfortunately, optimized analysis of different compounds requires specific extraction procedures in combination with specific analytical instrumentation. However, the most efficient extraction protocols often only cover a restricted number of compounds due to the different physico-chemical properties of these biological compounds. Consequently, comprehensive analysis of several molecular components like polar primary metabolites next to lipids or proteins require multiple aliquots to enable the specific extraction procedures required to cover these diverse compound classes. This multi-parallel sample handling of different sample aliquots is therefore not only more sample intensive, it also requires more time and effort to obtain the required extracts.ResultsTo circumvent large sample amounts, distributed into several aliquots for the comprehensive extraction of most relevant biological compounds, we developed a simple, robust and reproducible two-phase liquid–liquid extraction protocol. This one-step extraction protocol allows for the analysis of polar-, semi-polar and hydrophobic metabolites, next to insoluble or precipitated compounds, including proteins, starch and plant cell wall components, from a single sample. The method is scalable regarding the used sample amounts but also the employed volumes and can be performed in microcentrifuge tubes, enabling high throughput analysis. The obtained fractions are fully compatible with common analytical methods, including spectroscopic, chromatographic and mass spectrometry-based techniques. To document the utility of the described protocol, we used 25 mg of Arabidopsis thaliana rosette leaves for the generation of multi-omics data sets, covering lipidomics, metabolomics and proteomics. The obtained data allowed us to measure and annotate more than 200 lipid compounds, 100 primary metabolites, 50 secondary metabolites and 2000 proteins.ConclusionsThe described extraction protocol provides a simple and straightforward method for the efficient extraction of lipids, metabolites and proteins from minute amounts of a single sample, enabling the targeted but also untargeted high-throughput analyses of diverse biological tissues and samples.Electronic supplementary materialThe online version of this article (doi:10.1186/s13007-016-0146-2) contains supplementary material, which is available to authorized users.

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“…In both classes of light sensors, however, the nature of the downstream effectors that control the photoconductance has remained elusive. In invertebrates the gamut of proposed candidates includes IP 3 (31,32), Ca (33), DAG or metabolites thereof (34)(35)(36), PIP 2 (37), metabolic stress (38), capacitative Ca entry (39), protons (40), and mechanical forces (41). In fact, there are even indications that no universal mechanism may exists, because substances that exert a clear effect in one system, like PUFAs in Drosophila (35), proved inert in another, like Limulus (42), and vice versa (43).…”

Section: Discussionmentioning

confidence: 99%

“…Murine ipRGCs are only minimally affected by heparin and by thapsigargin (8), which argues against such notion; however, their light response was gradually abolished by buffering [Ca] i with BAPTA, although the onset of the effect was deemed too slow to represent direct interference with the messenger. Notwithstanding the likely ancestral relation between melanopsinbased photoreceptors of mammals and amphioxus, divergence of effector mechanisms is possible: there are precedents for photoreceptors of the same lineage in distantly related species (e.g., Drosophila vs. Limulus) seemingly recruiting different signaling elements to control the photoconductance, namely, the DAG (35,36) vs. the IP 3 branches (31-33) of the PLC cascade. Further work will be required to determine whether an extrapolation of the present conclusions to vertebrates is warranted, or whether separate melanopsin effector mechanisms have evolved, perhaps related to the widely different kinetics of the photoresponses in question.…”

Section: Discussionmentioning

confidence: 99%

Calcium activates the light-dependent conductance in melanopsin-expressing photoreceptors of amphioxus

Peinado

Osorno

Gómez

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Melanopsin, the photopigment of the "circadian" receptors that regulate the biological clock and the pupillary reflex in mammals, is homologous to invertebrate rhodopsins. Evidence supporting the involvement of phosphoinositides in light-signaling has been garnered, but the downstream effectors that control the lightdependent conductance remain unknown. Microvillar photoreceptors of the primitive chordate amphioxus also express melanopsin and transduce light via phospholipase-C, apparently not acting through diacylglycerol. We therefore examined the role of calcium in activating the photoconductance, using simultaneous, high timeresolution measurements of membrane current and Ca 2+ fluorescence. The light-induced calcium rise precedes the onset of the photocurrent, making it a candidate in the activation chain. Moreover, photolysis of caged Ca elicits an inward current of similar size, time course and pharmacology as the physiological photoresponse, but with a much shorter latency. Internally released calcium thus emerges as a key messenger to trigger the opening of lightdependent channels in melanopsin-expressing microvillar photoreceptors of early chordates.melanopsin | calcium | photoreceptor | amphioxus

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Diacylglycerol Activates the Light-Dependent Channel TRP in the Photosensitive Microvilli ofDrosophila melanogasterPhotoreceptors

Cited by 44 publications

References 36 publications

Amphioxus photoreceptors - insights into the evolution of vertebrate opsins, vision and circadian rhythmicity

Amphioxus photoreceptors - insights into the evolution of vertebrate opsins, vision and circadian rhythmicity

Protocol: a fast, comprehensive and reproducible one-step extraction method for the rapid preparation of polar and semi-polar metabolites, lipids, proteins, starch and cell wall polymers from a single sample

Calcium activates the light-dependent conductance in melanopsin-expressing photoreceptors of amphioxus

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