Diacylglycerol and Triacylglycerol as Responses in a Dual Response Surface-Optimized Process for Diacylglycerol Production by Lipase-Catalyzed Esterification in a Pilot Packed-Bed Enzyme Reactor

Abstract

Diacylglycerol (DAG) and triacylglycerol (TAG) as responses on optimization of DAG production using a dual response approach of response surface methodology were investigated. This approach takes the molecular equilibrium of DAG into account and allows for the optimization of reaction conditions to achieve maximum DAG and minimum TAG yields. The esterification reaction was optimized with four factors using a central composite rotatable design. The following optimized conditions yielded 48 wt % DAG and 14 wt % TAG: reaction temperature of 66.29 degrees C, enzyme dosage of 4 wt %, fatty acid/glycerol molar ratio of 2.14, and reaction time of 4.14 h. Similar results were achieved when the process was scaled up to a 10 kg production in a pilot packed-bed enzyme reactor. Lipozyme RM IM did not show any significant activity losses or changes in fatty acid selectivity on DAG synthesis during the 10 pilot productions. However, lipozyme RM IM displayed higher selectivity toward the production of oleic acid-enriched DAG. The purity of DAG oil after purification was 92 wt %.

Solvent-free enzymatic synthesis of 1, 3-Diacylglycerols by direct esterification of glycerol with saturated fatty acids

“…With attractive intermediates for synthetic application, pure 1, 3-diacylglycerols (1, 3-DAG) have been considered to be significant surfactants in food, cosmetics and pharmaceutical industries [1,2], as well as the effect on obesity prevention. 1, 3-DAG may potentially function on building blocks for synthesis of lipid derivatives, such as phospholipids, glycolipids or lipoproteins, which have been shown to improve bioavailability and reduce side effects, so as to used as starting materials for the preparation of some drugs [3-5].…”

Preparation of diacylglycerol‐enriched palm olein by phospholipase A1‐catalyzed partial hydrolysis

“…In some studies on production of DAG, separation of DAG from TAG was not conducted due to the low vapor pressure difference between them [12,14]. However, with a high temperature (over 220 7C) under very low pressure (,1 Pa), DAG can be separated from TAG by MD to obtain a product with high purity [21].…”

Preparation of Diacylglycerol-enriched Rice Bran Oil by Lipase-catalyzed Deacidification in Packed-bed Reactors by Continuous Dehydration

“…It can also improve the reaction efficiency and supply an efficient and simple method in industrial production. Numerous works have validly confirmed its advantage based on continuous enzyme reaction in production of MAG 23 , DAG 24 , TAG 24 and human milk fat substitutes 25 . Recycling of the enzyme was also easier in the continuous system.…”