Diagnoses and immature stages of some Australian<i>Tanytarsus</i>van der Wulp (Diptera: Chironomidae)

Ekrem, Torbjørn

doi:10.1046/j.1440-6055.2001.00246.x

Cited by 11 publications

(6 citation statements)

References 15 publications

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“…There are even species with larvae and pupae in marine or terrestrial environments[ 28 ]. The genus was erected by van der Wulp [ 29 ] and various species groups and regionally distributed species have been revised over the last few decades [ 30 – 37 ]. A morphological determination of some Tanytarsus species group can be extremely challenging.…”

Section: Introductionmentioning

confidence: 99%

Exploring Genetic Divergence in a Species-Rich Insect Genus Using 2790 DNA Barcodes

2015

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DNA barcoding using a fragment of the mitochondrial cytochrome c oxidase subunit 1 gene (COI) has proven to be successful for species-level identification in many animal groups. However, most studies have been focused on relatively small datasets or on large datasets of taxonomically high-ranked groups. We explore the quality of DNA barcodes to delimit species in the diverse chironomid genus Tanytarsus (Diptera: Chironomidae) by using different analytical tools. The genus Tanytarsus is the most species-rich taxon of tribe Tanytarsini (Diptera: Chironomidae) with more than 400 species worldwide, some of which can be notoriously difficult to identify to species-level using morphology. Our dataset, based on sequences generated from own material and publicly available data in BOLD, consist of 2790 DNA barcodes with a fragment length of at least 500 base pairs. A neighbor joining tree of this dataset comprises 131 well separated clusters representing 121 morphological species of Tanytarsus: 77 named, 16 unnamed and 28 unidentified theoretical species. For our geographically widespread dataset, DNA barcodes unambiguously discriminate 94.6% of the Tanytarsus species recognized through prior morphological study. Deep intraspecific divergences exist in some species complexes, and need further taxonomic studies using appropriate nuclear markers as well as morphological and ecological data to be resolved. The DNA barcodes cluster into 120–242 molecular operational taxonomic units (OTUs) depending on whether Objective Clustering, Automatic Barcode Gap Discovery (ABGD), Generalized Mixed Yule Coalescent model (GMYC), Poisson Tree Process (PTP), subjective evaluation of the neighbor joining tree or Barcode Index Numbers (BINs) are used. We suggest that a 4–5% threshold is appropriate to delineate species of Tanytarsus non-biting midges.

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Section: Introductionmentioning

confidence: 99%

Exploring Genetic Divergence in a Species-Rich Insect Genus Using 2790 DNA Barcodes

2015

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“…The taxon has generally been referred to as Tanytarsus fuscithorax in Western Australian literature . In the present study however, the taxon has been referred to as Tanytarsus fuscithorax/semibarbitarsus because Ekrem (2001) suggested that the immatures of T. fuscithorax can easily be confused with those of T. semibarbitarsus and that the two are probably closely related. The species is common in permanent lentic waters throughout Australia (Edward 1986) and is known to inhabit shallow pools in the south-west of Western Australia .…”

Section: Invertebrate Taxa In Relation To the Ph Groupsmentioning

confidence: 86%

Not really white - again: performing Jewish difference in Hollywood films since the 1980s

Stratton¹

2001

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“…The genus Tanytarsus was created by van der Wulp (1874) and species of Tanytarsus have been revised over the past decades (e.g. Cranston, 2000;Ekrem, 2001a;Ekrem, 2001b;Ekrem, 2002;Ekrem et al, 2003;Giłka & Paasivirta, 2009;Glover, 1973;Lin et al, 2018a;Reiss & Fittkau, 1971;Sanseverino, 2006;Sasa, 1980;Sasa, 1983;Sasa & Kawai, 1987), and their evolutionary history has been clarified recently by a molecular phylogenetic study based on multiple loci (Lin et al, 2018c). However, there remain challenges limiting the taxonomic disentangling of Tanytarsus in China: (a) there are still many poorly investigated regions, such as eastern Palearctic and Oriental regions; (b) life stages are incomplete as rearing is difficult; and (c) species boundaries remain uncertain in morphologically determined species.…”

Section: Introductionmentioning

confidence: 99%

The first comprehensive DNA barcode reference library of Chinese Tanytarsus (Diptera: Chironomidae) for environmental DNA metabarcoding

et al. 2020

Diversity and Distributions

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Aim Reliable DNA barcode reference libraries are critical for biodiversity monitoring based on Environmental DNA (eDNA). DNA barcoding has proven successful in species delimitation and association of life stages in many groups of animals, such as non‐biting midges (Chironomidae). We use the genus Tanytarsus van der Wulp, 1874, second largest genus of the family Chironomidae, to test whether DNA barcodes can be used to identify unknown and cryptic species. A comprehensive DNA barcode library will be built to facilitate further eDNA studies for biodiversity monitoring and conservation in freshwater ecosystems. Location Mainland China. Methods In this study, the cytochrome c oxidase subunit 1 (COI) DNA barcodes from 298 individuals, representing 56 morphospecies from China, were analysed, including 136 sequences from previous studies and 162 newly generated DNA barcodes. 298 COI barcodes were applied into the Automatic Barcode Gap Discovery (ABGD) to compare the operational taxonomic units (OTU) number resulting from the Barcode Index Number (BIN) based “Barcode Gap Analysis” and neighbour joining (NJ) tree. Results Total 298 barcode sequences were applied to 66 BINs, including 20 singleton BINs and 46 concordant BINs. ABGD yielded 55 OTUs with a prior intraspecific divergence of Pmax = 3.59%. By Integrating DNA barcodes with morphology, we revealed 21 putative species new to science and two unidentified species from the 56 well‐separated morphospecies. Besides, several cryptic species are uncovered using the NJ tree and TCS haplotype network based on COI DNA barcodes. Main conclusions Our results demonstrate that DNA barcodes enable the discovery and identification of Tanytarsus non‐biting midges. A comprehensive DNA barcode reference library could speed up accurate species delimitation and discovery by providing a reliable resource for taxonomic studies, and improve the application of eDNA metabarcoding in biomonitoring of freshwater ecosystems.

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Diagnoses and immature stages of some AustralianTanytarsusvan der Wulp (Diptera: Chironomidae)

Cited by 11 publications

References 15 publications

Exploring Genetic Divergence in a Species-Rich Insect Genus Using 2790 DNA Barcodes

Exploring Genetic Divergence in a Species-Rich Insect Genus Using 2790 DNA Barcodes

Not really white - again: performing Jewish difference in Hollywood films since the 1980s

The first comprehensive DNA barcode reference library of Chinese Tanytarsus (Diptera: Chironomidae) for environmental DNA metabarcoding

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