Diagnosing bioremediation of crude oil-contaminated soil and related geochemical processes at the field scale through microbial community and functional genes

Abstract

Purpose: Bioremediation is widely considered the most desirable procedure for remediation of oil-contaminated soil. Few studies have focused on the relationships among microbial community, functional genes of biodegradation, and geochemical processes during field bioremediation, which provide crucial information for bioremediation. Methods: In the current study, the microbial community and functional genes related to hydrocarbon and nitrogen metabolism, combined with the soil physico-chemical properties, were used to diagnose a set of bioremediation experiments, including bioaugmentation, biostimulation, and phytoremediation, at the field scale. Result: The results showed that the added nutrients stimulated a variety of microorganisms, including hydrocarbon degradation bacteria and nitrogen metabolism microorganisms. The functional genes reflected the possibility of aerobic denitrification in the field, which may be helpful in biodegradation. Biostimulation was found to be the most suitable of the studied bioremediation methods in the field. Conclusion:We offer a feasible approach to obtain useful bioremediation information and assist with the development of appropriate remediation procedures. The findings improve our knowledge of the interactions between microorganisms and edaphic parameters.

Diversity of hydrocarbon-degrading bacteria in Pulau Pari and their potential roles for bioremediation

“…Novosphingobium has been shown in other study to contribute substantially to the abundance of many hydrocarbonoclastic genes [12]. Sphingopyxis is known to be associated with variations in hydrocarbon degradation and oxidation of reduced sulfur compounds which are present in crude oil [13]. This study also shows that many isolates belonged this group is able to use hydrocarbon (hexadecane, phenanthrene, and dibenzothiophene) as the sole carbon sources (Table 2).…”

Insights into Bacterial Community Involved in Bioremediation of Aged Oil-Contaminated Soil in Arid Environment

“…A number of studies recently have been focused on application of next generation sequencing to discover microbial populations in environmental samples. 45 - 51 The work presented here focused on understanding the bacterial community structure associated with TPHs and PAHs degradation in desert soil at various stages of bioremediation process.…”

“…As a result, this genus can be used to predict the end of contamination in the oil-contaminated soils in the hot desert gulf region. 27 , 28 A recent study described Zhoui species isolated from mangrove sediment from East Asia as underexplored bacteria with lignocellulose degrading abilities. 81 Thus, the abundance of Zhoui at the end of treatment could be associated to the derivate with less complex ring structures resulting from the degradation of aromatic compounds with complex structure.…”

“…26 These bacteria could be used as oil contamination indicators. 27,28 Although cultivation of these bacteria is the classic methodology for assessing microbial community changes during the bioremediation process, less than 1% of environmental bacteria are cultivable. Recently, culture-independent techniques have been successfully applied as new approach to investigate the diversity of soil microbial communities.…”

Sustainable mitigation of heavy metals from effluents: Toxicity and fate with recent technological advancements

“…Silicate-modified biochar improves specific surface area (~45–112%), porosity of biochar (~5–12%), and internal Cd diffusion on biochar. As per XPS and FTIR analysis the prominent mechanism involved in Cd sorption and heavy metal irradiation process were Na + ion exchange, surface precipitation (CdSiO3 or Cd2SiO4, CdCO3), C = C π electrons coordination, and complexation of carboxyl and C-Si-O groups [ 103 ].…”