Diagnosing dengue virus infection: rapid tests and the role of micro/nanotechnologies

Zhang, Bei; Salieb-Beugelaar, Georgette B.; Nigo, Maurice M; Weidmann, Manfred; Hunziker, Patrick

doi:10.1016/j.nano.2015.05.009

Cited by 46 publications

(31 citation statements)

References 144 publications

(123 reference statements)

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“…After collecting beads for 5 min, the channels are washed with phosphate buffered saline (Figure 3c-4). The overall concentration time for this device is only 10 min, while traditional bio-sample preparation for RT-PCR can take up to 48 min and carries a higher contamination risk [59]. The major improvement in the concentration time is due to the use of a magnetic field, which is the active element of this concentration technique.…”

Section: Electric and Magnetic Fieldsmentioning

confidence: 99%

Recent Advances in Nanoparticle Concentration and Their Application in Viral Detection Using Integrated Sensors

Dincau

Yong-Kuk

Kim

et al. 2017

Sensors

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Early disease diagnostics require rapid, sensitive, and selective detection methods for target analytes. Specifically, early viral detection in a point-of-care setting is critical in preventing epidemics and the spread of disease. However, conventional methods such as enzyme-linked immunosorbent assays or cell cultures are cumbersome and difficult for field use due to the requirements of extensive lab equipment and highly trained personnel, as well as limited sensitivity. Recent advances in nanoparticle concentration have given rise to many novel detection methodologies, which address the shortcomings in modern clinical assays. Here, we review the primary, well-characterized methods for nanoparticle concentration in the context of viral detection via diffusion, centrifugation and microfiltration, electric and magnetic fields, and nano-microfluidics. Details of the concentration mechanisms and examples of related applications provide valuable information to design portable, integrated sensors. This study reviews a wide range of concentration techniques and compares their advantages and disadvantages with respect to viral particle detection. We conclude by highlighting selected concentration methods and devices for next-generation biosensing systems.

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Section: Electric and Magnetic Fieldsmentioning

confidence: 99%

Recent Advances in Nanoparticle Concentration and Their Application in Viral Detection Using Integrated Sensors

Dincau

Yong-Kuk

Kim

et al. 2017

Sensors

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“…There are four distinct but antigenically related serotypes of the virus that causes dengue, viz. DENV1, DENV2, DENV3 and DENV4 [36]. Infection by any of these serotypes may cause illnesses in individuals, encompassing classical dengue fever (DF), fatal dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS) [36][37][38][39].…”

Section: Denguementioning

confidence: 99%

Diagnosis of communicable diseases using papepr microfluidic platforms

Kumar¹,

Bhushan²,

Bhattacharya³

2017

Point-of-Care Diagnostics - New Progresses and Perspectives

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“…Rapid disease diagnostic tests are however required for monitoring infections such as hepatitis, dengue, etc. [6][7][8][9] Furthermore, developing countries often lack infrastructure as well as properly trained personnel to provide accurate diagnosis [10,11]. Recently, the concept of "lab on paper" has generated significant interest for nucleic acid assaying due to its ease of use and cost effectiveness, especially for applications in resource limited settings [12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Pixelated colorimetric nucleic acid assay

Aydın

Cheema

Ammanath

et al. 2020

Talanta

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A B S T R A C TConjugated polyelectrolytes (CPEs) have been widely used as reporters in colorimetric assays targeting nucleic acids. CPEs provide naked eye detection possibility by their superior optical properties however, as concentration of target analytes decrease, trace amounts of nucleic acid typically yield colorimetric responses that are not readily perceivable by naked eye. Herein, we report a pixelated analysis approach for correlating colorimetric responses of CPE with nucleic acid concentrations down to 1 nM, in plasma samples, utilizing a smart phone with an algorithm that can perform analytical testing and data processing. The detection strategy employed relies on conformational transitions between single stranded nucleic acid-cationic CPE duplexes and double stranded nucleic acid-CPE triplexes that yield distinct colorimetric responses for enabling naked eye detection of nucleic acids. Cationic poly[N,N,N-triethyl-3-((4-methylthiophen-3-yl)oxy)propan-1-aminium bromide] is utilized as the CPE reporter deposited on a polyvinylidene fluoride (PVDF) membrane for nucleic acid assay. A smart phone application is developed to capture and digitize the colorimetric response of the individual pixels of the digital images of CPE on the PVDF membrane, followed by an analysis using the algorithm. The proposed pixelated approach enables precise quantification of nucleic acid assay concentrations, thereby eliminating the margin of error involved in conventional methodologies adopted for interpretation of colorimetric responses, for instance, RGB analysis. The obtained results illustrate that a ubiquitous smart phone could be utilized for point of care colorimetric nucleic acids assays in complex matrices without requiring sophisticated software or instrumentation.

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Diagnosing dengue virus infection: rapid tests and the role of micro/nanotechnologies

Cited by 46 publications

References 144 publications

Recent Advances in Nanoparticle Concentration and Their Application in Viral Detection Using Integrated Sensors

Recent Advances in Nanoparticle Concentration and Their Application in Viral Detection Using Integrated Sensors

Diagnosis of communicable diseases using papepr microfluidic platforms

Pixelated colorimetric nucleic acid assay

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