Diagnosis and Detection of Seed-Borne Fungal Phytopathogens

Gupta, A. K.; Srivastava, Seweta; Devi, G. Lalitha; Singh, Vaibhav K.; Goswami, Sanjay Kumar; Gurjar, Malkhan Singh; Aggarwal, Rashmi

doi:10.1007/978-981-32-9046-4_5

Cited by 20 publications

(12 citation statements)

References 108 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hop latent viroid can be detected using molecular techniques 22,23,72 . Methods for detecting seed‐borne pathogens on cannabis or hemp seeds can be adapted from the vast literature on seed‐borne pathogens 65,73 . Since there is currently no certification program for cannabis or hemp in Canada, licensed producers cannot be guaranteed disease‐free planting materials.…”

Section: Disease Mitigation Approachesmentioning

confidence: 99%

“… 22 , 23 , 72 Methods for detecting seed‐borne pathogens on cannabis or hemp seeds can be adapted from the vast literature on seed‐borne pathogens. 65 , 73 Since there is currently no certification program for cannabis or hemp in Canada, licensed producers cannot be guaranteed disease‐free planting materials. As a result, spread of Fusarium through cuttings provided by propagators, for example, is not uncommon, resulting in introduction of the pathogen into new areas.…”

Section: Disease Mitigation Approachesmentioning

confidence: 99%

See 1 more Smart Citation

Emerging diseases of Cannabis sativa and sustainable management

Punja

2021

Pest Management Science

114

View full text Add to dashboard Cite

Cultivation of cannabis plants (Cannabis sativa L., marijuana) has taken place worldwide for centuries. In Canada, legalization of cannabis in October 2018 for the medicinal and recreational markets has spurned interest in large‐scale growing. This increased production has seen a rise in the incidence and severity of plant pathogens, causing a range of previously unreported diseases. The objective of this review is to highlight the important diseases currently affecting the cannabis and hemp industries in North America and to discuss various mitigation strategies. Progress in molecular diagnostics for pathogen identification and determining inoculum sources and methods of pathogen spread have provided useful insights. Sustainable disease management approaches include establishing clean planting stock, modifying environmental conditions to reduce pathogen development, implementing sanitation measures, and applying fungal and bacterial biological control agents. Fungicides are not currently registered for use and hence there are no published data on their efficacy. The greatest challenge remains in reducing microbial loads (colony‐forming units) on harvested inflorescences (buds). Contaminating microbes may be introduced during the cultivation and postharvest phases, or constitute resident endophytes. Failure to achieve a minimum threshold of microbes deemed to be safe for utilization of cannabis products can arise from conventional and organic cultivation methods, or following applications of beneficial biocontrol agents. The current regulatory process for approval of cannabis products presents a challenge to producers utilizing biological control agents for disease management. © 2021 The Author. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

show abstract

Section: Disease Mitigation Approachesmentioning

confidence: 99%

Section: Disease Mitigation Approachesmentioning

confidence: 99%

Emerging diseases of Cannabis sativa and sustainable management

Punja

2021

Pest Management Science

114

View full text Add to dashboard Cite

show abstract

“…BIO-PCR assay is a modification of end-point PCR technique which involves a pre-assay incubation step in a diseased sample to increase the biomass of the causal agent. This technique is mainly used to concentrate target pathogens by growing the target pathogen in a growing media that prevent the growth of non-target microorganisms to improve detection ( Schaad et al., 1995 ) and has been effectively used to detect seed-borne fungal pathogens ( Kumar et al., 2020 ). A seed and airborne lupin anthracnose disease caused by Colletotrichum lupini was diagnosed using the BIO-PCR method.…”

Section: Molecular Tools For Detection Of Fungimentioning

confidence: 99%

Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review

Hariharan¹,

Prasannath²

2021

Front. Cell. Infect. Microbiol.

118

View full text Add to dashboard Cite

Phytopathogenic fungal species can cause enormous losses in quantity and quality of crop yields and this is a major economic issue in the global agricultural sector. Precise and rapid detection and identification of plant infecting fungi are essential to facilitate effective management of disease. DNA-based methods have become popular methods for accurate plant disease diagnostics. Recent developments in standard and variant polymerase chain reaction (PCR) assays including nested, multiplex, quantitative, bio and magnetic-capture hybridization PCR techniques, post and isothermal amplification methods, DNA and RNA based probe development, and next-generation sequencing provide novel tools in molecular diagnostics in fungal detection and differentiation fields. These molecular based detection techniques are effective in detecting symptomatic and asymptomatic diseases of both culturable and unculturable fungal pathogens in sole and co-infections. Even though the molecular diagnostic approaches have expanded substantially in the recent past, there is a long way to go in the development and application of molecular diagnostics in plant diseases. Molecular techniques used in plant disease diagnostics need to be more reliable, faster, and easier than conventional methods. Now the challenges are with scientists to develop practical techniques to be used for molecular diagnostics of plant diseases. Recent advancement in the improvement and application of molecular methods for diagnosing the widespread and emerging plant pathogenic fungi are discussed in this review.

show abstract

“…Molecular diagnostic techniques based on the detection of fungal DNA have been widely used for species‐specific detection of Colletotrichum associated with soybean anthracnose. PCR is the method of choice in the field of molecular diagnosis of soybean pathogens, as it enables an exponential amplification of the target DNA sequence, making it a fast, efficient, and attractive technique (Kumar et al., 2020). Using these molecular techniques, tiny amounts of host samples are sufficient for the detection of Colletotrichum in soybean seeds or other plant tissues.…”

Section: Identification and Molecular Diagnosticsmentioning

confidence: 99%

Soybean anthracnose caused by Colletotrichum species: Current status and future prospects

Boufleur

Ciampi‐Guillardi

Tikami

et al. 2021

Molecular Plant Pathology

View full text Add to dashboard Cite

Soybean (Glycine max) is one of the most important cultivated plants worldwide as a source of protein‐rich foods and animal feeds. Anthracnose, caused by different lineages of the hemibiotrophic fungus Colletotrichum, is one of the main limiting factors to soybean production. Losses due to anthracnose have been neglected, but their impact may threaten up to 50% of the grain production. Taxonomy While C. truncatum is considered the main species associated with soybean anthracnose, recently other species have been reported as pathogenic on this host. Until now, it has not been clear whether the association of new Colletotrichum species with the disease is related to emerging species or whether it is due to the undergoing changes in the taxonomy of the genus. Disease symptoms Typical anthracnose symptoms are pre‐ and postemergence damping‐off; dark, depressed, and irregular spots on cotyledons, stems, petioles, and pods; and necrotic laminar veins on leaves that can result in premature defoliation. Symptoms may evolve to pod rot, immature opening of pods, and premature germination of grains. Challenges As accurate species identification of the causal agent is decisive for disease control and prevention, in this work we review the taxonomic designation of Colletotrichum isolated from soybean to understand which lineages are pathogenic on this host. We also present a comprehensive literature review of soybean anthracnose, focusing on distribution, symptomatology, epidemiology, disease management, identification, and diagnosis. We consider the knowledge emerging from population studies and comparative genomics of Colletotrichum spp. associated with soybean providing future perspectives in the identification of molecular factors involved in the pathogenicity process. Useful website Updates on Colletotrichum can be found at http://www.colletotrichum.org/. All available Colletotrichum genomes on GenBank can be viewed at http://www.colletotrichum.org/genomics/.

show abstract

Diagnosis and Detection of Seed-Borne Fungal Phytopathogens

Cited by 20 publications

References 108 publications

Emerging diseases of Cannabis sativa and sustainable management

Emerging diseases of Cannabis sativa and sustainable management

Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review

Soybean anthracnose caused by Colletotrichum species: Current status and future prospects

Contact Info

Product

Resources

About