Diagnosis and screening of abnormal hemoglobins

Sani, Ali; Idrees Khan, Muhammad; Shah, Saud; Tian, Youli; Zha, Genhan; Fan, Liuyin; Zhang, Qiang; Cao, Chengxi

doi:10.1016/j.cca.2023.117685

Cited by 5 publications

(1 citation statement)

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“…The goal is to craft a method that is not only efficient but also addresses the drawbacks of existing techniques, ensuring practicality and affordability in its application . Fluorescence and electrochemical analyses, for instance, involve intricate and time-consuming processes utilizing blood samples, incorporating biochemical and physical steps for Hb detection through fluorescent and electrochemical signals, respectively. , Spectrophotometry, in contrast, relies on optical direct detection, observing monochromatic light intensity changes caused by Hb or its derivatives’ absorption, following the Lambert–Beer law. Recent years have seen the emergence of optical-based detection methods, such as photoacoustic spectrometry, dynamic spectrometry, and spectral imaging, garnering significant interest from researchers.…”

Section: Introductionmentioning

confidence: 99%

Selective Extraction and Quantification of Hemoglobin Based on a Novel Molecularly Imprinted Nanopolymeric Structure of Poly(acrylamide-vinyl imidazole)

Şarkaya,

Özçelik,

Yaşar

et al. 2024

ACS Omega

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Imbalances in hemoglobin (Hb) levels can lead to conditions such as anemia or polycythemia, emphasizing the importance of precise Hb extraction from blood. To address this, a novel synthetic imprinted polymer was meticulously developed for capturing and separating Hb. Poly(acrylamide-vinylimidazole) nanopolymer (poly(AAm-VIM)) was synthesized using acrylamide and vinyl imidazole as functional monomers through surfactant-free emulsion polymerization. Characterization using FTIR, particle size, zeta potential, and SEM ensured the polymer's structure. The Hb-imprinted nanopolymer (Hbpoly(AAm-VIM)) demonstrated notable specificity, with a calculated Hb-specific adsorption value (Q max ) of 3.7377 mg/g in a medium containing 2.5 mg/mL Hb. The molecularly imprinted polymer (MIP) exhibited approximately 5 times higher Hb adsorption than the nonimprinted polymer (NIP). Under the same conditions, the imprinted nanopolymer displayed 2.39 and 2.17 times greater selectivity for Hb over competing proteins such as bovine serum albumin (BSA) and lysozyme (Lys), respectively. Also, SDS-PAGE analysis results confirmed the purification of Hb by the molecularly imprinted nanopolymer. These results underscore the heightened specificity and efficacy of the molecularly imprinted nanopolymer in selectively targeting Hb atoms among other proteins. Incorporating such polymers is justified by their notable affinity, cost-effectiveness, and facile production. This research contributes valuable insights into optimizing synthetic imprinted polymers for efficient Hb extraction, with potential in medical diagnostics and treatment applications.

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Section: Introductionmentioning

confidence: 99%