Diagnosis and Therapeutic Relevance of Micrometastases

Holz, E; Pantel, Klaus; Riethmüller, Gert

doi:10.1007/978-3-642-71967-7_18

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…A number of publications have focused on the role of disseminated tumor cells in recent years, and the term ''minimal residual disease'' is well established [23]. Although the prognostic value of circulating tumor cells is controversial, several studies have shown that the presence of minimal residual disease is of prognostic value for the course of the disease and the prognosis after surgical resection [3][4][5][9][10][11].…”

Section: Discussionmentioning

confidence: 99%

Correlation of CK‐20‐Positive Cells in Peripheral Venous Blood with Serum CEA Levels in Patients with Colorectal Carcinoma

et al. 2007

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Tumor cell dissemination appears to be an early event in tumor progression, and tumor cells can be detected in peripheral venous blood at the time of the operation. Although cytokeratin 20 (CK-20) is not specifically expressed by colorectal carcinomas, it represents a widely used marker for the detection of colorectal tumor cells. We used the combination of density centrifugation and CK-20 real-time reverse transcription polymerase chain reaction to detect CK-20-positive cells in the peripheral venous blood of 37 patients with colorectal carcinoma. Detection rates were compared to serum levels of the tumor markers carcinoembryonic antigen (CEA), carbohydrate antigen CA 19-9, and cancer antigen CA 125. The prognostic impact was assessed by the overall survival and by univariate and multivariate analysis. Overall, CK-20-positive cells in peripheral venous blood were detected in 11 of 37 (29.7%) patients. CK-20-positive patients showed a significantly higher mean serum CEA level (90.3 ng/ml) than the 4.1 ng/ml found in the CK-20-negative group (p = 0.03). CEA levels also correlated with CK-20 copy numbers. No significant correlation was observed for CA 19-9 or CA 125. CK-20-negative patients showed a trend toward better survival (p = 0.08). In the univariate analysis, CA 19-9, CEA, tumor size, lymph node status, grading, the presence of distant metastases, and resection status reached significant prognostic levels, whereas the detection of CK-20-positive cells showed only a prognostic trend (p = 0.06). Multivariate analysis failed to identify independent prognostic parameters. Here we report the correlation of CK-20-positive cells in peripheral venous blood with the serum CEA level of patients with colorectal cancer, which may represent a potential marker of the tumor load.

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Section: Discussionmentioning

confidence: 99%

Correlation of CK‐20‐Positive Cells in Peripheral Venous Blood with Serum CEA Levels in Patients with Colorectal Carcinoma

et al. 2007

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“…These findings suggest that the dissemination of tumor cells occurs before or during the removal of the tumor, and that mi-crometastases that cannot be detected by conventional histopathological means might be present at the time of resection. This hypothesis led to the establishment of the term minimal residual disease [3,4]. Although the prognostic value of circulating tumor cells is controversial several studies have shown that the presence of minimal residual disease influences the course of disease and the prognosis after surgical resection [5][6][7][8].…”

mentioning

confidence: 99%

Prognostic Impact of CK‐20‐positive Cells in Peripheral Venous Blood of Patients with Gastrointestinal Carcinoma

et al. 2005

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Despite curative tumor resection, about 30%-50% of patients with locally advanced gastrointestinal (GI) carcinoma develop tumor recurrence which may be caused by pre- or intraoperative tumor cell dissemination. We examined the combination of optimized density gradient centrifugation with a CK-20 reverse transcriptase-polymerase chain reaction to detect and quantify circulating tumor cells in peripheral blood. Peripheral venous blood (20 ml) of patients with GI carcinomas was collected during primary tumor staging before and after the endoscopy procedure. CK-20 expression in peripheral venous blood was found in 22 of 82 patients (26.8%) with a nonsignificant difference between the upper GI tract (23.9%) and the lower GI tract (30.5%). The correlation with clinical outcome (24-month-survival) revealed a significantly worse prognosis (p < 0.05) of CK-20-positive patients with carcinoma of the upper GI tract and a trend toward a worse prognosis for patients with carcinoma of the lower GI tract. Quantification of CK-20 expression in peripheral blood showed a significantly higher circulating CK-20 copy number (median: 2816) in patients with metastatic tumors than in those with non-metastatic tumors (median: 983) (p < 0.05). For a subset of 42 primarily operated patients, we correlated the detection rate with UICC (International Union Against Cancer) staging categories. In contrast to the upper GI tract, the detection rate of patients with carcinoma of the lower GI tract showed a trend toward tumor size (pT) and a significant correlation with the presence of distant metastases (pM) (p < 0.01) and the postoperative residual tumor status (R) (p < 0.01). The endoscopy procedure did not lead to an increased detection of CK-20 expression.

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“…Mafosfamide purging of autologous marrow grafts from patients with acute myeloid leukaemia does not need a control of leukaemia cell depletion because leukaemic cells are killed but not removed from the graft. However, detection of small populations of AML cells is possible in the case of certain chromosomal translocations by PCR or FISH, or in some phenotypes by two-colour flow cytometry [71,72].…”

Section: Quality Control Of Cell Selection and Minimal Residual Diseasementioning

confidence: 99%

Cell Selection in Marrow and Stem Cell Transplantation

Krüger¹,

Gutensohn²,

Tögel³

et al. 1999

Transfus Med Hemother

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High-dose therapy with subsequent allogeneic or autologous transplantation of haemopoietic progenitor cells has proved to be an important therapy for patients with haematological malignancies, certain non-haematological cancers and several nonmalignant disorders such as severe aplastic anaemia. Allogeneic and autologous transplantation are associated with specific complications. One major problem after allotransplantation is the graft versus host disease with jaundice, diarrhoea and erythema. The major problem of autologous stem cell transplantation is a potential reinfusion of contaminating tumor cells. Allogeneic transplantation has been refined, and an approach to modulate graft versus host disease is a partial or complete lymphocyte depletion. Especially in the allogeneic setting, the field of cell therapy for modulation of graft versus host disease and treatment of transplant-related complications is expanding. For autologous transplantation different techniques to purge autografts from malignant contaminants have been developed. These cell selection techniques consist of immunological, biological, cultural, chemotherapeutical and physical techniques. This article reviews techniques currently used for graft engineering in bone marrow and blood stem cell transplantation.

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Diagnosis and Therapeutic Relevance of Micrometastases

Cited by 4 publications

References 15 publications

Correlation of CK‐20‐Positive Cells in Peripheral Venous Blood with Serum CEA Levels in Patients with Colorectal Carcinoma

Correlation of CK‐20‐Positive Cells in Peripheral Venous Blood with Serum CEA Levels in Patients with Colorectal Carcinoma

Prognostic Impact of CK‐20‐positive Cells in Peripheral Venous Blood of Patients with Gastrointestinal Carcinoma

Cell Selection in Marrow and Stem Cell Transplantation

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