Diagnosis and therapy of rare central nervous system infections

Qi, Xiaokun

doi:10.4103/2347-8659.135568

Cited by 4 publications

(4 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Infeksi SSP dapat disebabkan oleh infeksi bakteri, virus, jamur dan parasit. 7 Parasit dapat bermanifestasi dengan gejala yang ringan sampai dengan berat. 6,8 Infeksi dengan gejala yang tidak spesifik menyebabkan, diagnosis dapat menjadi sulit.…”

Section: Konteks: Outline Modul Mooc Cns Parasitic Infectionunclassified

“…Pada kebanyakan kasus, para ahli dan dokter sulit untuk menegakkan diagnosis pasti termasuk ke arah pengobatannya. 7 Pendekatan dengan karakteristik epidemiologi dasar serta membedakan temuan hasil radiografi dapat meningkatkan kemungkinan deteksi dan pengobatan yang tepat dari infeksi parasit pada sistem saraf pusat. 9 Modul CNS Parasitic Infection telah diajarkan dalam Kurikulum Prodi S1 Kedokteran FKKMK UGM yang sebelumnya dilakukan dengan tatap muka penuh.…”

Section: Konteks: Outline Modul Mooc Cns Parasitic Infectionunclassified

See 1 more Smart Citation

Students’ Perception on the Use of a Massive Open Online Course for Parasitology: A Preliminary Study

Utomo,

Kusumasari,

Murhandarwati

et al. 2023

JPKI

View full text Add to dashboard Cite

Background: The COVID-19 pandemic had a significant impact on medical education globally. The learning process has been shifted to online learning to ensure health and safety measures. Online learning approaches and applications are also established rapidly, including the Massive Open Online Course (MOOC), which can support learning in many topics and facilitate a large number of students effectively and efficiently. Department of Parasitology and Department of Medical Education and Bioethics, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada developed the Central Nervous System (CNS) Parasitic Infection module as an online learning for both undergraduate and postgraduate levels.Aims: The study aimed to evaluate and explore students’ perception of the CNS Parasitic Infection online module development in an MOOC format.Methods: This is an evaluation study using an explorative qualitative approach. The pilot implementation of the CNS Parasitic Infection module was evaluated using in-depth interviews with ten (10) students of the Master in Tropical Medicine and the Master in Biomedical Science programs. The participants were requested to share their learning experience on the module, and their suggestions for module improvement. The interviews were conducted online using Zoom. The interview transcripts were analyzed using thematic analysis.Results: Implementing the CNS Parasitic Infection module using MOOC asynchronous approach received both positive and negative responses from participants. A total of 6 themes were identified from participants, for instance, learning outcomes achievement, interactive e-case design, learning flexibility, course timing, network issues, and the need for learning directions.Conclusion: CNS Parasitic Infection can be delivered to students using an asynchronous MOOC format. Students’ outcomes achievement was sufficient using this method, and overall, students appreciated the implementation of the program.

show abstract

Section: Konteks: Outline Modul Mooc Cns Parasitic Infectionunclassified

Students’ Perception on the Use of a Massive Open Online Course for Parasitology: A Preliminary Study

Utomo,

Kusumasari,

Murhandarwati

et al. 2023

JPKI

View full text Add to dashboard Cite

show abstract

“…Fungal identification can be done by fungal internal transcribed spacer region or ribosomal small subunit (18S) rDNA PCR and amplicon sequencing [9,11]. Detection of mycobacterial infection is commercially available, such as Cobas Taqman MTB test and Xpert MTB/RIF assays [12,13]. However, these methods are expensive and require special equipment or service, which are not suitable for laboratories with limited resources available.…”

Section: Introductionmentioning

confidence: 99%

Universal Multiplex Polymerase Chain Reaction-Restriction Fragment Length polymorphism (UMPCR-RFLP) for Rapid Detection and Species Identification of Fungal and Mycobacterial Pathogens

Szekely

Chelae

Ingviya

et al. 2020

Walailak J Sci & Tech

View full text Add to dashboard Cite

Fungal and mycobacterial skin infections are common in immunocompromised patients and patients with febrile neutropenia, since the patients’ ability to control localized infection is diminished by the disease. The similarity of the lesions caused by these organisms conduces to difficulty of differential diagnosis. Although a histopathological examination and a microbial culture are standard methods for laboratory diagnosis of skin infection, the methods have drawbacks. Histopathological examination yields low positive results, while microbial culture is time-consuming and might result in no growth, causing delayed treatment. This study aimed to develop and evaluate an in-house rapid polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the detection and identification of fungal and mycobacterial pathogens in resource-limited laboratories. A total of 26 typed species of human pathogenic fungi and 12 species of mycobacteria were used. Strain differentiation was analyzed by using multiplex PCR-RFLP. The internal transcript spacer region (ITS) of fungi and heat-shock protein 65 (hsp65) gene of mycobacteria were amplified using ITS1-ITS4 and Tb11-Tb12 universal primers, respectively. The RFLP patterns were examined at genus-specific and species-specific level. No cross-amplification was observed between fungal and mycobacterial tested strains, nor any specific binding between primers and human DNA. It was concluded that the multiplex PCR-RFLP method developed in this study can be used as a molecular diagnostic test for fungal and mycobacterial species identification. In the future, this technique may be useful for detecting fungal and mycobacterial infection directly from clinical specimens.

show abstract

“…Fungal infection is generally opportunistic and has gradually increased in recent years, mainly due to the increase in acquired immunodeficiency syndrome (AIDS), transplant surgery and drug resistance to antifungal medication. Cryptococcus neoformans infection is still the most common CNS fungal infection, whereas Aspergillus and Mucor are relatively uncommon causes of CNS infection (Qi, 2014 ). Aspergillosis CNS fungal infection may present with various features depending on the pathogenicity of a specific pathogenic species and the status of the host's immune response (Rallis et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%