Diagnosis and Treatment of Gonorrhea in the Female

Neisseria gonorrhoeae identified by the direct fluorescent antibody technique in male contacts.

“…Lucas, Price, Thayer, and Schroeter (1967) have shown in a study in vitro that killed N. gonorrhoeae are not all lysed and that strong fluorescent antibody staining may be observed up to 24 days after exposure to bactericidal concentrations of penicillin. In this study only two of the ten FA-positive patients had been treated with antibiotics during the preceding 6 months.…”

Diagnosis of gonorrhoea by culture on a selective medium containing vancomycin, colistin, nystatin and trimethoprim (VCNT). A comparison with Gram-staining and immunofluorescence.

“…However, in a few cases, organisms in every way characteristic of gonococci by immunofluorescence fail to grow, just as do organisms typical of gonococci in Gramstained smears of samples from both men and women. For these reasons, it is not surprising that with somewhat different techniques some workers have found that delayed immunofluorescence yields more positive results than culture on media of various types (Fry and Wilkinson 1964); Lucas, Price, and Thayer, 1967), that some have found the results of immunofluorescence and culture on selective media to correspond very closely (Lind, 1969), and that others have had more positive results from culture than from immunofluorescence (Martin, Peacock, and Thayer, 1965;McGill, Moffett, Masterton, and Schofield, 1969). In addition to this, Schmale, Martin, and Domescik (1969) found that cultures were negative at the first examination in 6 to 8 per cent.…”

Value of vaginal and rectal cultures in the diagnosis of gonorrhoea. With special reference to areas with limited medical facilities.

“…Direct plating was used in most of the papers referred to although, in the work of Wilkinson (1965) and Roepstorff and Hammarstrom (1966), the specimens were kept initially in a transport medium and inoculated on to plates after a maximum of 24 hours. Double plating of the samples was carried out by Wilkinson (1965), Roepstorff and Hammarstrom (1966) and Lucas, Price, Thayer, and Schroeter (1967) and, as emphasized in the last two papers, this probably contributed to the lower yield reported from the urethra in these series (66 per cent. in both).…”