Purpose Urban and peri-urban agriculture is becoming increasingly important as a source of income and food for the urban population in South Africa. While most studies on urban agriculture have focused their attention on surface soils, there is dearth of information regarding subsoil properties. This study examined properties of subsoil horizons that may impede root growth and productivity of crops under urban agriculture. Materials and methods The properties of topsoil (0-20 cm) and subsoil horizons (20-40 cm) of four profiles from plots within the city of Mahikeng (25°48′ S and 25°38′ E) were examined to determine the nature of subsoil constraints that can limit root growth and crop productivity. The plots were selected in an area extending through four residential suburbs of the city, and two plots with a long history of cultivation were purposely selected from each suburb to represent the main cropping systems and soil types. Soil physical (penetrometer resistance, bulk density, hydraulic conductivity), chemical (pH, exchangeable Ca, Mg, K, Na, phosphorus and boron) and biological (root growth, organic carbon, microbial biomass, enzyme activity) properties were measured in the profiles. Results and discussion Even though there was a large variability between profiles, the results revealed high bulk density (mean 2.06 Mg m −3