Diagnosis of anthelmintic resistance in cattle in Brazil: A comparison of different methodologies

Neves, José Henrique das; Carvalho, Nadino; Rinaldi, Laura; Cringoli, Giuseppe; Amarante, Alessandro Francisco Talamini do

doi:10.1016/j.vetpar.2014.10.015

Cited by 48 publications

(38 citation statements)

References 24 publications

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“…More recently, Bayesian modelling using Markov chain Monte Carlo (MCMC) methods have been advocated as robust statistical analyses to cope with low and aggregated FEC data (Denwood et al., 2010, Torgerson et al., 2014). These MCMC-based procedures, available as open-source R packages or web-interface software, are being increasingly used to infer drug efficacy and to monitor AR in horse nematodes (Denwood et al., 2010, Fischer et al., 2015) and cattle helminths (Neves et al., 2014, O'Shaughnessy et al., 2014, Geurden et al., 2015, Novobilský and Höglund, 2015, Ramos et al., 2016). However, the performance of these MCMC procedures with the low mean FEC and parasite aggregation levels commonly found in cattle has not yet been evaluated.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of ivermectin against gastrointestinal nematodes of cattle in Denmark evaluated by different methods for analysis of faecal egg count reduction

Pena-Espinoza

Thamsborg

Denwood

et al. 2016

International Journal for Parasitology: Drugs and Drug Resistan

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The efficacy of ivermectin (IVM) against gastrointestinal nematodes in Danish cattle was assessed by faecal egg count reduction test (FECRT). Six cattle farms with history of clinical parasitism and avermectin use were included. On the day of treatment (Day 0), 20 naturally infected calves per farm (total n = 120) were stratified by initial faecal egg counts (FEC) and randomly allocated to a treatment group dosed with 0.2 mg IVM kg−1 body weight s.c. (IVM; n = 10) or an untreated control group (CTL; n = 10). Individual FEC were obtained at Day 0 and Day 14 post-treatment and pooled faeces by group were cultured to isolate L3 for detection of Ostertagia ostertagi and Cooperia oncophora by qPCR. Treatment efficacies were analysed using the recommended WAAVP method and two open-source statistical procedures based on Bayesian modelling: ‘eggCounts’ and ‘Bayescount’. A simulation study evaluated the performance of the different procedures to correctly identify FEC reduction percentages of simulated bovine FEC data representing the observed real data. In the FECRT, reduced IVM efficacy was detected in three farms by all procedures using data from treated animals only, and in one farm according to the procedures including data from treated and untreated cattle. Post-treatment, O. ostertagi and C. oncophora L3 were detected by qPCR in faeces of treated animals from one and three herds with declared reduced IVM efficacy, respectively. Based on the simulation study, all methods showed a reduced performance when FEC aggregation increased post-treatment and suggested that a treatment group of 10 animals is insufficient for the FECRT in cattle. This is the first report of reduced anthelmintic efficacy in Danish cattle and warrants the implementation of larger surveys. Advantages and caveats regarding the use of Bayesian modelling and the relevance of including untreated cattle in the FECRT are discussed.

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Section: Introductionmentioning

confidence: 99%

Efficacy of ivermectin against gastrointestinal nematodes of cattle in Denmark evaluated by different methods for analysis of faecal egg count reduction

Pena-Espinoza

Thamsborg

Denwood

et al. 2016

International Journal for Parasitology: Drugs and Drug Resistan

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“…The resistance of D. hominis to ivermectin is a very important issue because this drug is used extensively on Brazilian cattle (Neves et al, 2014). It is assumed that, with the continued use of the product, resistance to it will spread across the country, with negative consequences to the leather industry.…”

Section: Discussionmentioning

confidence: 99%

“…The animals were weighed and separated into groups in order to evaluate the efficacy of anthelmintics (Neves et al, 2014). The drugs were administered to 10 animals in each group, given by subcutaneous injection following the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

“…In February and March 2013, during an experiment conducted to evaluate anthelmintic resistance in cattle (Neves et al, 2014), we observed, by visual inspection, that most of the animals of two farms were presenting the typical lesions on skin caused by D. hominis infestation. For this reason, we decided to also evaluate the efficacy of macrocyclic lactones against D. hominis larvae in the cattle on those farms; one located in the municipality of Pardinho and one in the municipality Anhembi, both in the state of São Paulo, Brazil.…”

Section: Cattle Herds and Managementmentioning

confidence: 99%

“…However, there have been frequent reports of anthelmintic resistance (Neves et al, 2014), and also reports of R. microplus (Reck et al, 2014) and Cochliomyia hominivorax (Lopes et al, 2013) resistance to treatment with macrocyclic lactones. This paper describes, for the first time, the presence of live D. hominis larvae after the treatment of cattle with ivermectin and moxidectin in Brazil.…”

Section: Introductionmentioning

confidence: 99%

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Dermatobia hominis : Potencial risk of resistance to macrocyclic lactones

Neves

Carvalho

Amarante

2015

Veterinary Parasitology

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Dermatobia hominis is an ectoparasite that infests various species of mammals, including cattle, impairing the quality of cowhides and leather. After observing natural infestation with D. hominis larvae in cattle on two farms in the state of São Paulo, Brazil, we evaluated the efficacy of two macrocyclic lactones, ivermectin and moxidectin, against this parasite. The drugs were administered to 10 animals in each group, following the manufacturer's instructions. The groups were: Group 1-treated with ivermectin (0.2mg/kg of body weight (BW)); Group 2-treated with moxidectin (0.2mg/kg BW); and Group 3-control (untreated). On the farm in Pardinho, a total of 12 and 16 live larvae were found in 6 and in 8 animals 10 days after the treatment with ivermectin and moxidectin, respectively, while in the control group 4 bovines had a total of 7 live larvae. On the farm in Anhembi, 2, 4 and 6 live larvae were extracted from ivermectin, moxidectin and control groups, respectively, after the treatment. This is the first report of the presence of live D. hominis larvae after the treatment of cattle with ivermectin and moxidectin in Brazil.

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Occurrence, Measurement and Clinical Perspectives of Drug Resistance in Important Parasitic Helminths of Livestock

Woodgate

Cornell

Sangster

2017

Antimicrobial Drug Resistance

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Diagnosis of anthelmintic resistance in cattle in Brazil: A comparison of different methodologies

Cited by 48 publications

References 24 publications

Efficacy of ivermectin against gastrointestinal nematodes of cattle in Denmark evaluated by different methods for analysis of faecal egg count reduction

Efficacy of ivermectin against gastrointestinal nematodes of cattle in Denmark evaluated by different methods for analysis of faecal egg count reduction

Dermatobia hominis : Potencial risk of resistance to macrocyclic lactones

Occurrence, Measurement and Clinical Perspectives of Drug Resistance in Important Parasitic Helminths of Livestock

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