Diagnosis of biofilm-associated infections in medical devices

Bose, Seema; Ghosh, Atindra Krishna

doi:10.1533/9780857097224.1.71

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…Therefore, zebrafish models using <120 hpf embryos have low ethical considerations [65]. Moreover, similar to C. elegans and D. melanogaster, zebrafish larvae microinjection tests have a short timeframe, low maintenance cost and high throughput [52] which are major advantages in virulence testing [48].…”

Section: Discussionmentioning

confidence: 99%

“…An optimal animal model used for virulence testing can be characterized by having a low cost, high infection success, ease of infection initiation, a low mortality rate of the test organism and a reasonable expense [48]. To meet these requirements, the incubation time, bacterial concentrations and droplet size of the newly developed protocol had to be optimized to reach a high infection success with relatively low mortality in a short period of time with easy implementation and, at the same time, to have a clear, statistically significant difference compared to the untreated control group.…”

Section: Optimization Of the Combined Zebrafish Microinjection Virule...mentioning

confidence: 99%

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Evaluating the In Vivo Virulence of Environmental Pseudomonas aeruginosa Using Microinjection Model of Zebrafish (Danio rerio)

Kaszab,

Jiang,

Szabó

et al. 2023

Antibiotics

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(1) Background: Microinjection of zebrafish (Danio rerio) embryos offers a promising model for studying the virulence and potential environmental risks associated with Pseudomonas aeruginosa. (2) Methods: This work aimed to develop a P. aeruginosa infection model using two parallel exposition pathways on zebrafish larvae with microinjection into the yolk and the perivitelline space to simultaneously detect the invasive and cytotoxic features of the examined strains. The microinjection infection model was validated with 15 environmental and clinical strains of P. aeruginosa of various origins, antibiotic resistance profiles, genotypes and phenotypes: both exposition pathways were optimized with a series of bacterial dilutions, different drop sizes (injection volumes) and incubation periods. Besides mortality, sublethal symptoms of the treated embryos were detected and analyzed. (3) Results: According to the statistical evaluation of our results, the optimal parameters (dilution, drop size and incubation period) were determined. (4) Conclusions: The tested zebrafish embryo microinjection infection model is now ready for use to determine the in vivo virulence and ecological risk of environmental P. aeruginosa.

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Section: Discussionmentioning

confidence: 99%

Section: Optimization Of the Combined Zebrafish Microinjection Virule...mentioning

confidence: 99%