Diagnosis of bovine neosporosis

Dubey, J. P.; Schares, Gereon

doi:10.1016/j.vetpar.2006.03.035

Cited by 235 publications

(268 citation statements)

References 284 publications

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“…Previous studies carried out in Argentina using three bulls experimentally infected with 3 Â 10 7 N. caninum tachyzoites failed to detect specific antibodies in seminal plasma samples using an immunofluorescent antibody test (Campero et al, personal communication). Discrepancies between results could be explained by the higher sensitivity and specificity of the ELISA technique used [22]. Our results suggest that detection of specific antibodies to N. caninum in seminal plasma by ELISA, which is especially useful in bulls from AI centres, could be an alternative to serology for the diagnosis of bovine neosporosis.…”

Section: Discussionmentioning

confidence: 88%

“…In recent studies it has been shown that it is necessary for bovine semen to contain between 10 2 and 10 3 tachyzoites in order to induce an infection in athymic BALB/c nu/nu mice (our unpublished data). However, the susceptibility of nude mice to N. caninum is well documented [22] and the athymic mouse model has been used satisfactorily for Neospora isolation from bovine brain in our laboratory (unpublished data).…”

Section: Discussionmentioning

confidence: 99%

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Experimental neosporosis in bulls: Parasite detection in semen and blood and specific antibody and interferon-gamma responses

et al. 2007

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Aim: To investigate the presence of Neospora caninum in semen and blood, and the development of specific antibody and interferon-gamma (IFN-g) responses in experimentally infected bulls. Methods: Eight bulls were intravenously infected with 10 8 live N. caninum tachyzoites of NC-1 isolate. The presence of N. caninum in semen and blood was assessed using a nested-PCR procedure. PCR-positive semen samples were bioassayed using a BALB/c nu/nu mouse model. Specific anti-N. caninum antibody and IFN-g responses were also examined. In parallel, eight seronegative bulls were studied as non-infected controls. All bulls were monitored for 26 weeks. Results: All eight experimentally infected bulls showed N. caninum DNA in their semen and/or blood samples at some time during the course of the study. Parasite load in semen ranged from 0.1 to 14.5 parasites/ml (mean 6.0). N. caninum could not be detected in BALB/c nu/nu mice inoculated with PCR-positive semen samples. A significant increase in mean serum specific IgM antibody response to N. caninum was detected between 10 and 28 days post-infection (p.i.). Serum specific IgG, IgG1, and IgG2 antibody levels in experimentally infected bulls were significantly different after 21, 10, and 14 days p.i. as compared to controls, respectively. Specific anti-N. caninum IgG were detected in seminal plasma from infected bulls and values obtained were different from controls after 25 days p.i. Mean specific IFN-g responses in experimentally infected bulls were significantly higher than controls 3 days p.i. Conclusions: This is the first study to report the presence of N. caninum DNA in the semen and blood of experimentally infected bulls. Our observations indicate an intermittent presence of N. caninum in low numbers in semen and associated with chronic stage of the infection. This study is also the first to report the detection of anti-N. caninum IgG in seminal plasma of experimentally infected bulls. #

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Experimental neosporosis in bulls: Parasite detection in semen and blood and specific antibody and interferon-gamma responses

et al. 2007

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“…Outras alterações patológicas consistem na reabsorção ou mumificação fetal e ainda, nascimento com aspecto clínico normal porém cronicamente infectado HOAR et al, 1996;SCHARES, 2006). O aparecimento dos sintomas em bezerros nascidos de mães infectadas ocorre em cerca de cinco dias após o parto , enquanto observou ainda mielite, miocardite e encefalite associado ao N. caninum, em bezerros infectados.…”

Section: Neosporose Em Bovinosunclassified

Neosporose em Animais Domésticos

Guimarães

Romanelli

2006

Sem. Ci. Agr.

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ResumoA neosporose, doença emergente causada pelo Neospora caninum, é considerada como a principal causa de abortamentos em bovinos em vários países. Estudos têm sido realizados até hoje com diversas espécies de animais, visando melhor elucidação da neosporose considerando agente, hospedeiro e meio ambiente. Esta revisão enfatiza aspectos sobre patogenia e diagnóstico da neosporose em alguns animais domésticos. Palavras-chave: Neospora caninum. Bovinos. Cães. Ovinos. Eqüídeos. AbstractNeosporosis, emerging disease caused by Neospora caninum was considered the most important causes of aborts in cattle from many countries. Some studies have been developed in several animal species to understand host-parasite relationship. The present review describes some aspects about pathogeny and diagnostic of the neosporosis in domestic animals.

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“…Others studies have compared different ELISAs, based on the use of recombinant N. caninum-purified antigens, with the IFAT. 10 In cattle, previous studies 4,13 showed perfect agreement between ELISA and IFAT when the recombinant Ncp29 (NcsSRS1) antigen was used. ELISAs based on the use of rNcGra6 and NcGra7 also showed good correlation with the IFAT.…”

mentioning

confidence: 98%

“…Different methods were developed, including indirect fluorescent antibody test (IFAT), 7 direct agglutination, Western blot, or enzyme-linked immunosorbent assays (ELISAs). 10 Whole tachyzoites 18 or purified antigens 15 also can be used for the detection of specific antibodies. Because intact tachyzoites are used as antigen in the IFAT, this test mainly detects antibodies directed to antigens present on the cell surface of the parasite.…”

mentioning

confidence: 99%

Evaluation of a SRS2 Sandwich Commercial Enzyme-Linked Immunosorbent Assay for the Detection of Anti-Neospora Caninum Antibodies in Bovine and Canine Sera

et al. 2009

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Abstract. Neospora caninum is a parasite responsible for abortion in cows and neuromuscular disease in dogs. Serology is the most widely used technique to evaluate the prevalence of N. caninum in different host populations. A sandwich enzyme-linked immunosorbent assay (ELISA) based on the use of an anti-SRS2 monoclonal antibody was evaluated against the indirect fluorescent antibody test for 100 canine sera and against a well-characterized ELISA for 102 bovine sera. In cattle sera, the relative sensitivity and relative specificity were 100%. In dog sera, the relative specificity and relative sensitivity were 94% and 86%, respectively. The kappa value was 1 for bovine sera and 0.77 for canine sera. The seroprevalence was 3.9% in bovine sera and 21-23% in canine sera. The SRS2 sandwich ELISA was considered a valuable tool in both species.

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Diagnosis of bovine neosporosis

Cited by 235 publications

References 284 publications

Experimental neosporosis in bulls: Parasite detection in semen and blood and specific antibody and interferon-gamma responses

Experimental neosporosis in bulls: Parasite detection in semen and blood and specific antibody and interferon-gamma responses

Neosporose em Animais Domésticos

Evaluation of a SRS2 Sandwich Commercial Enzyme-Linked Immunosorbent Assay for the Detection of Anti-Neospora Caninum Antibodies in Bovine and Canine Sera

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