Diagnosis of brucellosis in sheep and goats raw milk by fast and reliable techniques

Abstract

This study aimed to evaluate the occurrence of brucellosis in sheep and goats' raw milk samples by detecting anti-Brucella antibodies and Brucella species at Erbil governorate. A total of 320 raw milk samples (150 sheep milk and 170 goats milk) were irregularly collected from dairy females. The overall occurrence of Brucella antibodies in samples of sheep and goats raw milk was 11.6% and 9.7% according to MRT and indirect ELISA, respectively. Isolation of Brucella (B.) species from samples of raw milk was 7.8%. The isolated species of Brucella were B. melitensis and B. abortus. A noticeable increase in frequency during September to November was observed. In conclusion, brucellosis is still a considerable public health threat in the Erbil. Based on the tests performance, the study recommends MRT in standard observing of brucellosis in milk aggregate farms, centers, and dairy manufactories. Customers are also recommended to adequately pasteurize the milk in order to damage this milk-borne pathogen before ingestion or saleable handling.

Histopathological and histochemical study of mastitis in sheep

“…The interaction between milk leukocytes and pathogenic bacteria resulting in the establishment of inflammation (24), then macrophage release chemoattractant that attracted other polymorphonuclear cells from vasculature to the site of infection and accumulated in large numbers around the alveoli, neutrophil emigration occurs through gaps in the lining epithelia, or due to sloughing apart of it this inflammation and emigration of neutrophil are associated with sloughing or damage of the alveoli and epithelial ducts cell (25) these result agreement with (26,27), vacuolar and coagulative necrosis can results from damage of the mammary vasculature after infection with pathogenic bacteria and these result agreement with (28,29). Chronic mastitis pathology showed severe infiltration of mono morphonuclear cells.…”

Isolation and identification of <em>Helicobacter pylori</em> from raw chicken meat in Dhamar Governorate, Yemen

“…Two hundred and sixty (260) fresh raw chicken meat samples (140 Thigh, and 120 Breast), were collected from retail markets and chicken slaughterhouses in different places of Dhamar Governorate, from July to December 2020. The samples were put in sterile cooled polyethylene bags and kept in ice box with temperature approximately 4°C during transport and storage at the laboratory (Almashhadany, 2021b ). The bacteriological analysis was performed within 2 h of sample collection.…”

Isolation and Identification of Helicobacter Pylori from RTE Foods

“…In the laboratory, the isolation of H. pylori was done under aseptic conditions as previously published [41,48]. Briefly, samples were cut into small pieces using sterile blades to liberate adherent bacteria.…”