2003
DOI: 10.1354/vp.40-1-32
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Diagnosis of Canine Lymphoid Neoplasia Using Clonal Rearrangements of Antigen Receptor Genes

Abstract: Abstract. Although the diagnosis of canine leukemia and lymphoma in advanced stages is usually uncomplicated, some presentations of the disease can be a diagnostic challenge. In certain situations, lymphoma and leukemia can be difficult to distinguish from a benign reactive proliferation of lymphocytes. Because clonality is the hallmark of malignancy, we have developed an assay that uses the polymerase chain reaction to amplify the variable regions of immunoglobulin genes and T-cell receptor genes to detect th… Show more

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Cited by 302 publications
(394 citation statements)
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“…PARR was performed as described previously [1,3]. Briefly, each sample was amplified by two sets of primers (major and minor) for IgH and one set of primers for TCRγ.…”
Section: Methodsmentioning
confidence: 99%
“…PARR was performed as described previously [1,3]. Briefly, each sample was amplified by two sets of primers (major and minor) for IgH and one set of primers for TCRγ.…”
Section: Methodsmentioning
confidence: 99%
“…Here, we provide the first report of two molecular techniques that confirm the diagnosis of mature B cell lymphoma/leukemia in horses. Assessments of B cell IGHV clonality and sequencing have widely been used in human patients and infrequently in dogs with lymphoid neoplasia (leukemia and lymphoma) (5,39,40). Rearrangement of the IGHV region and expression of a functional B cell receptor occur during B cell development in the bone marrow, and mutations may follow during antigen-dependent development (41).…”
Section: Discussionmentioning
confidence: 99%
“…However, this contradicted the clinical finding in the peripheral blood (PB), which showed CLL. Therefore, a polymerase chain reaction for antigen receptor rearrangement (PARR) analysis (Kahotechno, Fukuoka, Japan) of the LN and PB samples was performed to detect the clonal expansion of lymphocytes [2]. Both samples exhibited rearrangements of the immunoglobulin heavy chain (IgH) gene (B cells) and the T cell receptor (TCR) γ gene (T cells) (Fig.…”
mentioning
confidence: 99%
“…Both samples exhibited rearrangements of the immunoglobulin heavy chain (IgH) gene (B cells) and the T cell receptor (TCR) γ gene (T cells) (Fig. 3A, B) [2]. We also examined the immunophenotype of the LN sample using flow cytometry.…”
mentioning
confidence: 99%
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