Diagnosis of Canine Viral Infections

Guy, James S.

doi:10.1016/s0195-5616(86)50133-9

Cited by 10 publications

(5 citation statements)

References 11 publications

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“…The virus is now an epidemic enteric pathogen of dogs worldwide. 4 It has a single-stranded DNA genome length of about 5,200 nucleotides, with 2 promoters. The virus expresses 2 structural (VP1 and VP2) and 2 non-structural (NS1 and NS2) proteins through alternative splicing of viral mRNAs.…”

mentioning

confidence: 99%

Detection of Canine Parvovirus in Fecal Samples using Loop-Mediated Isothermal Amplification

2006

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Loop-mediated isothermal amplification (LAMP) is a novel, sensitive, and rapid technique for detection of genomic DNA. The end-product of the technique is a white precipitate of magnesium pyrophosphate that is visible without the use of gel electrophoresis. The LAMP method was applied to the detection of canine parvovirus (CPV) genomic DNA. A set of 4 primers, 2 outer and 2 inner, were designed from CPV genomic DNA targeting the VP2 gene. The optimal reaction time and temperature for LAMP were determined to be 60 minutes and 63 degrees C. On the basis of results for 50 canine fecal samples using polymerase chain reaction (PCR) analysis as the gold standard, the relative sensitivity of LAMP was 100% and the relative specificity was 76.9%. The detection limit of the LAMP method was 10(-1) median tissue culture infective doses (TCID50)/ml, compared with 10 TCID50/ml for PCR analysis. In addition to the advantage resulting from visual detection of the end product, the LAMP method is very rapid, requiring only 1 hour to complete. This assay would be a viable alterative to PCR analysis for diagnosis of CPV infection in dogs. The LAMP method holds promise for use as a diagnostic assay for CPV detection in a clinical setting.

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mentioning

confidence: 99%

Detection of Canine Parvovirus in Fecal Samples using Loop-Mediated Isothermal Amplification

2006

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“…It has been reported that anti‐CDV IgM antibody can be induced by CDV vaccination (Guy 1986, Blixenkrone‐Moller and others 1991, Barben and others 1999). No CDV vaccine antigen has previously been detected in the serum, although it has been detected in lymph tissues (Blixenkrone‐Moller 1989).…”

Section: Discussionmentioning

confidence: 99%

“…The disease can be diagnosed by measuring the titre of anti‐CDV antibody in a dog's serum (Guy 1986, Greene and Appel 1998), but the titre can be low or undetectable in recently infected dogs and in dogs that are fatally infected (Krakowka and others 1975, Miele and Krakowka 1983). In such cases, an ELISA for CDV antigen has been described as a diagnostic method (Potgieter and Ajidagba 1989, Gemma and others 1996) which may improve the rate of accurate diagnosis.…”

mentioning

confidence: 99%

Detection of canine distemper virus antigen in canine serum and its application to diagnosis

Soma

Ishii²,

Hara

et al. 2003

Veterinary Record

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Canine distemper virus (CDV) antigen was detected in the serum of dogs by an ELISA and the results of this assay were compared with an anti-CDV immunoglobulin M (IgM) antibody test. In paired sera from 26 naturally infected dogs, the antigen-positive rate was 26.9 per cent at the first examination and 11.5 per cent at the second examination two to three weeks later. The antigen was detected in three of the 10 dogs which were negative for anti-CDV IgM antibody at the first examination. It could also be detected in the serum of between eight and two of 40 specific pathogen-free dogs vaccinated against CDV, for up to four weeks after they were vaccinated.

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“…However, it was difficult to tell the difference between this disease and other types of illnesses, such as kennel cough when the disease is in its early stages. Anti-CDV IgM antibody identification can be used to make a serologic diagnosis (8,9). However, owing to a detectable IgM antibody titer to CDV after 3 weeks of vaccination, it remains a problem in vaccinated pets (9).…”

Section: Introductionmentioning

confidence: 99%

“…Anti-CDV IgM antibody identification can be used to make a serologic diagnosis (8,9). However, owing to a detectable IgM antibody titer to CDV after 3 weeks of vaccination, it remains a problem in vaccinated pets (9).…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Canine Distemper Virus in Dogs in Baghdad Province, Iraq

Mansour

Hasso

2021

Iraqi J. Vet. Med.

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Canine distemper (CD) is an infectious disease that affects dogs and is extremely contagious and lethal, with a high mortality and morbidity rates. It infects a broad variety of animals, including primates, cetaceans, and carnivores causing a multi-systemic pathological condition. This study aimed to detect canine distemper virus (CDV) in blood samples of dogs clinically suspected with distemper at the Baghdad Veterinary Hospital, Baghdad, Iraq. CDV nucleoprotein gene (N) was detected in the whole blood of 46 dogs using reverse transcription-PCR (RT-PCR). The partially amplified (591 bp) fragment of the N gene was detected in 12 of 46 (26%) blood samples of dogs examined. Based on the partial sequencing data of the N gene, three local isolates might be similar to the NCBI-BLAST reference CDV virus isolates FJ977579.1 China, AF378705.1 USA, and AF305419.1 UK, while other strains EU072200.1 Hungary, AF164967.1 Switzerland, KU578257.1 Germany, and AB474397.1 Japan were found to be rather distinct. The isolates displayed a higher level of similarity with the Snyder Hill CDV strain and Onderstepoort CDV strain. There was less homology with the CDV strain A75/17 of Switzerland and 007Lm CDV strain of Japan. In conclusion, this study confirmed that CDV infection is present in domestic dogs in Iraq. This may indicate a risk of the disease spreading to parts of the country that may be disease-free.

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Diagnosis of Canine Viral Infections

Cited by 10 publications

References 11 publications

Detection of Canine Parvovirus in Fecal Samples using Loop-Mediated Isothermal Amplification

Detection of Canine Parvovirus in Fecal Samples using Loop-Mediated Isothermal Amplification

Detection of canine distemper virus antigen in canine serum and its application to diagnosis

Molecular Detection of Canine Distemper Virus in Dogs in Baghdad Province, Iraq

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