Diagnosis of Chlamydia infection in women

Su, Wen Hsiang; Tsou, Tsung Shan; Chen, Chien Sheng; Ho, Tien Yu; Lee, Wen Ling; Yu, Yuan Yi; Chen, Tien Jui; Tan, Chia Hsuan; Wang, Peng Hui

doi:10.1016/j.tjog.2011.07.001

Cited by 22 publications

(12 citation statements)

References 33 publications

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“…7 An easy, fast, inexpensive, high-throughput, and nano-scale-sensitive multiplexed detection system might be the answer. A clinician might be able to detect the different pathogens causing cervical and vaginal infections, including gonorrhoea or chlamydiasis, 37 immediately and accurately using a microarray-based immunoassay. With a sensitive test for the point-of-care setting, a correct prescription of antibiotics will be made rapidly and confidently and over-or undertreatment can be avoided.…”

Section: Prospecting Methodsmentioning

confidence: 99%

Are we satisfied with the tools for the diagnosis of gonococcal infection in females?

Tsou

Chen

et al. 2011

Journal of the Chinese Medical Association

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Gonorrhea (Neisseria gonorrhoeae) is a common sexually transmitted infection in women, with a heavy burden on female and neonatal health, because sequelae occur, such as female infertility, ectopic pregnancy, neonatal ophthalmitis and infection, and chronic pelvic pain. Prompt and appropriate antibiotic treatment can cure infection and avoid complications. However, adequate treatment is not easy, because early and rapid identification of gonorrhea is interfered with by many factors, including the complicated mixed microflora of the vagina and cervix, non-user-friendly culture systems, and lack of immediate availability of results, even with a combination of subjective complaint and high clinical suspicion. A PubMed search was conducted using the major headings of "gonorrhoea and diagnostic tool" and "Neisseria gonorrhoeae and diagnostic tool", before the end of 2010. Recently available methods for the diagnosis of gonorrhea infection in women were included, including traditional tools and advanced technology. Traditional tools such as microscopic examination and microbial culture have been used broadly; unfortunately, they have relatively lower specificity or sensitivity, and most importantly, "see-and-treat" is impossible for these infected women. Advances in technology, such as antigen detection by immunoassay and nucleic acid amplification tests (NAATs), have achieved major progress in the diagnosis of gonorrhea, because of their accuracy, convenience and time-saving aspects. However, NAATs are expensive, making their acceptance impossible in developing countries. Detection of pathogens including N. gonorrheae using microarray chips is viewed as a possible solution, because it is a relatively rapid, easy, inexpensive and sensitive tool, which makes an "identify-and-treat" or point-of-care policy possible. A rapid and affordable tool with high sensitivity and specificity for detection of gonorrhea in developing countries is still not available at the time of writing. To make a point-of-care policy possible, advanced technology for aiding diagnosis of gonorrhea is encouraged and appreciated.

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Section: Prospecting Methodsmentioning

confidence: 99%

Are we satisfied with the tools for the diagnosis of gonococcal infection in females?

Tsou

Chen

et al. 2011

Journal of the Chinese Medical Association

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“…For example, chlamydia (caused by the pathogen Chlamydia trachomatis, CT) is the most common sexually transmitted infection worldwide, with more than 110 million cases reported annually (4). Diagnosis of CT infections is challenged by a lack of standard symptoms (many infections are asymptomatic) (5) and the presence of mixed flora (particularly in the female reproductive tract) (6). Thus, rapid NAATs with high sensitivity and specificity are critically needed, especially NAATs that can deal with the high levels of host or background DNA likely to be present in clinical samples such as urine samples and swabs (7,8).…”

Section: Introductionmentioning

confidence: 99%

Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification

Rolando

Jue

Barlow

et al. 2020

Nucleic Acids Research

151

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Isothermal amplification assays, such as loopmediated isothermal amplification (LAMP), show great utility for the development of rapid diagnostics for infectious diseases because they have high sensitivity, pathogen-specificity and potential for implementation at the point of care. However, elimination of non-specific amplification remains a key challenge for the optimization of LAMP assays. Here, using chlamydia DNA as a clinically relevant target and high-throughput sequencing as an analytical tool, we investigate a potential mechanism of non-specific amplification. We then develop a real-time digital LAMP (dLAMP) with high-resolution melting temperature (HRM) analysis and use this single-molecule approach to analyze approximately 1.2 million amplification events. We show that single-molecule HRM provides insight into specific and non-specific amplification in LAMP that are difficult to deduce from bulk measurements. We use real-time dLAMP with HRM to evaluate differences between polymerase enzymes, the impact of assay parameters (e.g. time, rate or florescence intensity), and the effect background human DNA. By differentiating true and false positives, HRM enables determination of the optimal assay and analysis parameters that leads to the lowest limit of detection (LOD) in a digital isothermal amplification assay.

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“…Lamentablemente, Chile no cuenta con una recomendación al respecto pese a existir evidencias de una prevalencia similar a la reportada por varios de los países (E.U,A., Canadá, miembros de la comunidad europea) que ya han incorporado dicho tamizaje como parte de la vigilancia epidemiológica periódica 9,[26][27][28] . En la actualidad existe consenso sobre el método de tamizaje y tipo de muestras a analizar, tanto en mujeres como en hombres 9,29 . Este debe realizarse mediante técnicas de amplificación de ácidos nucleicos (TAAN) dada su alta sensibilidad y especificidad comparado con los métodos tradicionales (ej.…”

Section: Artículo Originalunclassified

“…Este debe realizarse mediante técnicas de amplificación de ácidos nucleicos (TAAN) dada su alta sensibilidad y especificidad comparado con los métodos tradicionales (ej. detección de inclusiones citoplasmáticas por citología, cultivo celular, detección de antígenos de C. trachomatis por ELISA o inmunofluorescencia) 22,[29][30][31][32][33] . En Chile, existen actualmente cinco estudios de corte transversal que han evaluado la prevalencia de esta infección utilizando TAAN, todos realizados antes del año 2013 [13][14][15][16][17] .…”

Section: Artículo Originalunclassified

La prevalencia actual de infección genital por Chlamydia trachomatis en adolescentes y mujeres jóvenes chilenas asintomáticas justifica la vigilancia periódica

Zamboni

Ralph

García

et al. 2016

Rev. chil. infectol.

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Diagnosis of Chlamydia infection in women

Cited by 22 publications

References 33 publications

Are we satisfied with the tools for the diagnosis of gonococcal infection in females?

Are we satisfied with the tools for the diagnosis of gonococcal infection in females?

Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification

La prevalencia actual de infección genital por Chlamydia trachomatis en adolescentes y mujeres jóvenes chilenas asintomáticas justifica la vigilancia periódica

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