Diagnosis of common hemoglobinopathies among South East Asian population using capillary isoelectric focusing system

Srivorakun, Hataichanok; Fucharoen, Goonnapa; Sanchaisuriya, Kanokwan; Fucharoen, Supan

doi:10.1111/ijlh.12585

Cited by 11 publications

(5 citation statements)

References 25 publications

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“…α -Thalassemia is prevalent in populations of Southeast Asia, Central Africa, and the Middle East [5, 6]. In the last few decades, massive population migrations favored the spread of these defects.…”

Section: Introductionmentioning

confidence: 99%

Alpha-Thalassemia in North Morocco: Prevalence and Molecular Spectrum

Laghmich

Ismaili

Barakat

et al. 2019

BioMed Research International

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Unlike the other hemoglobinopathies, few researches have been published concerning α-thalassemia in Morocco. The epidemiological features and the mutation spectrum of this disease are still unknown. This regional newborn screening is the first to study α-thalassemia in the north of Morocco. During the period from January 2015 to December 2016, 1658 newborns umbilical blood samples were investigated. Suspected newborns were screened for α-globin defects using Gap-PCR and Multiplex Ligation-dependent Probe Amplification technique. The prevalence of α-thalassemia, its mutation spectrum, and its allelic frequencies were described for the first time in Morocco. Six different α-globin genetic disorders were detected in 16 neonates. This screening valued the prevalence of α-thalassemia in the studied population at 0.96% and showed the wide mutation spectrum and the heterogeneous geographical distribution of the disease. A high rate of carriers was observed in Laouamra, a rural commune in Larache province. Heterogeneity of α-globin alleles in Morocco explains the high variability of α-thalassemia severity. This diversity reflects the anthropological history of the country. These results would contribute to the prevention of thalassemia in Morocco directing the design of a nationwide screening strategy and awareness campaign.

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Section: Introductionmentioning

confidence: 99%

Alpha-Thalassemia in North Morocco: Prevalence and Molecular Spectrum

Laghmich

Ismaili

Barakat

et al. 2019

BioMed Research International

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“…Other CE variations have been used in the past but within the time covered by this review only CIEF has been applied to the diagnosis of hemoglobinopathies by Srivorakun et al. . These investigators applied CIEF to blood samples obtained from 665 adults and 13 fetal blood samples at a routine thalassemia clinic.…”

Section: Applications In Different Clinical Fieldsmentioning

confidence: 99%

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

Phillips

2017

Electrophoresis

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Recent advances in CE and microchip-CE in clinical applications: 2014 to mid-2017CE and microchip CE (ME) are powerful tools for the analysis of a number of different analytes and have been applied to a variety of clinical fields and human samples. This review will present an overview of the most recent applications of these techniques to different areas of clinical medicine during the period of 2014 to mid-2017. CE and ME have been applied to clinical chemistry, drug detection and monitoring, hematology, infectious diseases, oncology, endocrinology, neonatology, nephrology, and genetic screening. Samples examined range from serum, plasma, and urine to lest utilized materials such as tears, cerebral spinal fluid, sweat, saliva, condensed breath, single cells, and biopsy tissue. Examples of clinical applications will be given along with the various detection systems employed.

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“…The deficiency in production of either the α - or β -chain of hemoglobin leads to α - or β -thalassemia, respectively 7 . Thalassemia affects approximately 5% of the world population and is more common in North and Central Africa, Southern Asia, and Mediterranean countries 8 , 9 , 10 . The level of functional hemoglobin indicates the severity of thalassemia; patients with moderate thalassemia have a moderate level of hemoglobin (9 – 12 g/dL) 8 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Significance of Tear Ferning Patterns in beta-Thalassemia Patients

Fagehi

Abbadi

Abusharha

et al. 2021

Klin Monbl Augenheilkd

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Aim This study was designed to evaluate the tear film in beta-thalassemia patients using tear ferning (TF) and phenol red thread (PRT) tests. Methods Forty beta-thalassemia patients, aged 18 – 38 years (25.7 ± 6.0 years), along with a control group of 40 age-matched subjects with healthy eyes completed the study. All subjects completed the ocular surface disease index (OSDI) first, followed by the TF and PRT tests with a 5-minute gap between the tests. Results Significant differences (Wilcoxon test, p < 0.05) were observed between the beta-thalassemia and control groups in all scores. Among beta-thalassemia patients, OSDI scores indicated eye dryness in 35 subjects (87.5%). PRT showed dryness in 31 subjects (77.5%) in the right and left eyes, and the TF grades were ≥ 2 for the tears collected from 35 subjects (87.5%). There was a strong correlation (r = 0.851, p = 0.001) between the PRT measurements in the right and left eyes. There was a medium correlation between OSDI and TF scores (r = 0.309, p = 0.026) and between OSDI and age (r = 0.365, p = 0.010). Age had a moderate negative correlation (r = − 0.328, p = 0.019) with the PRT obtained from the right eye. Conclusion Significant ocular dryness symptom was observed in beta-thalassemia patients, based on the scores collected from TF and PRT tests. Beta-thalassemia patients develop dry eyes, possibly due to several factors, including reduced tear secretion. Therefore, it is important to regularly evaluate both the quality and quantity of tears in patients with beta-thalassemia.

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Diagnosis of common hemoglobinopathies among South East Asian population using capillary isoelectric focusing system

Cited by 11 publications

References 25 publications

Alpha-Thalassemia in North Morocco: Prevalence and Molecular Spectrum

Alpha-Thalassemia in North Morocco: Prevalence and Molecular Spectrum

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

Evaluation of the Significance of Tear Ferning Patterns in beta-Thalassemia Patients

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