Diagnosis of cyanide poisoning using an automated, field-portable sensor for rapid analysis of blood cyanide concentrations

Bortey-Sam, Nesta; Jackson, Randy; Gyamfi, Obed A.; Bhadra, Subrata; Freeman, Caleb; Mahon, Sari B.; Brenner, Matthew; Rockwood, Gary A.; Logue, Brian A.

doi:10.1016/j.aca.2019.11.034

Cited by 9 publications

(4 citation statements)

References 21 publications

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“…After incubation, 25 μl was removed from the vial and used for the analysis. Quantification of HCN proceeded via active microdiffusion, chemical modification of HCN using NDA and taurine, and LC-MS/MS analysis of the CN-NDA-taurine compound as described 10,36,64,65 . Briefly, NDA (2 mM), taurine (100 mM), and NaOH (10 mM), 200 µl each, were added to the reagent chamber of a two-chamber sample preparation cartridge which allows active airflow from the sample chamber to the reagent chamber.…”

Section: Methodsmentioning

confidence: 99%

Endogenously produced hydrogen cyanide serves as a novel mammalian gasotransmitter

Zuhra,

Petrosino,

Janickova

et al. 2024

Preprint

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Small, gaseous molecules, known as gasotransmitters (NO, CO, H2S), are produced endogenously in mammalian cells and serve important biological roles. Hydrogen cyanide, traditionally considered a cytotoxic molecule in mammals, serves as an endogenous mediator in several plants and bacterial species. Here we show that low concentrations of cyanide are generated endogenously in mouse liver and human hepatocytes. Cyanide production is stimulated by glycine, occurs at the low pH of lysosomes and requires peroxidase activity. Cyanide, in turn, is detectable in several cellular compartments. Cyanide is also detectable basally in the blood of mice; its levels increase after treatment of the animals with glycine. Rhodanese activity regulates endogenous cyanide levels. Cyanide, when generated endogenously at an optimal level, exerts stimulatory effects on mitochondrial bioenergetics, cell metabolism and cell proliferation. Dysregulation of endogenous cyanide, either below or above optimal levels, impairs cellular bioenergetics. The regulatory effects of cyanide are in part mediated by posttranslational modification of cysteine residues via protein cyanylation; cyanylated protein residues can be detected basally, and increase after treatment with glycine. Controlled low-dose cyanide supplementation exhibits cytoprotective effects, as demonstrated in hypoxia and reoxygenation modelsin vitroandin vivo. However, pathologically elevated cyanide production, as demonstrated in nonketotic hyperglycinemia – an autosomal recessive disease of glycine metabolism – is deleterious to the cells.

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Section: Methodsmentioning

confidence: 99%

Endogenously produced hydrogen cyanide serves as a novel mammalian gasotransmitter

Zuhra,

Petrosino,

Janickova

et al. 2024

Preprint

Self Cite

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“…Cyanide toxicity is known because it can inhibit the mitochondrial cytochrome C oxidase and suppress oxygen transport. 23,24 Consequently, the development of sensitive and selective probes for the sensing of this anion has been gaining considerable attention in recent years because of its essential role in a wide variety of biological, 25 environmental, 26 biochemical, 27 clinical, 28 synthetical due to design and preparation of the respective chemosensors and industrial applications. 21,22 …”

Section: Introductionmentioning

confidence: 99%

Photophysical and anion sensing properties of a triphenylamine–dioxaborinine trimeric compound

et al. 2023

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“…However, large number of cyanide anion have been produced and used in various industrial processes such as electronic devices and organic polymer production, pesticides, pharmaceuticals, metalwork, and gold extraction. [4][5][6][7] Furthermore, in nature, microbes and plants such as bacteria, fungi, algae, fruit seeds, and sweet potatoes also defensively generate cyanogenic substances toward their enemies. [8] Because of this, simple and fast methods for CN À ion detection have been increasingly gained importance during the last decades.…”

Section: Introductionmentioning

confidence: 99%

“…They interact with respiratory enzymes, blocks cytochrome oxidase by binding to the Fe 3 + atom and cause hypoxia and interrupt the mitochondrial membrane, resulting in vomiting seizure, unconsciousness, and untimely death. [4] In living animals, including humans, even a small amount of cyanide is fatal. However, large number of cyanide anion have been produced and used in various industrial processes such as electronic devices and organic polymer production, pesticides, pharmaceuticals, metalwork, and gold extraction.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Highly Selective BODIPY Based Turn‐Off Colorimetric Cyanide Sensor**

Kaya

2021

ChemistrySelect

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Cyanide ion (CN À ion) is one of the most harmful inorganic pollutants wasted by various industrial processes. Although some spectroscopic methods are being used, these methods need advanced instrumentation and have high detection limits. Because of this, rapid, low-cost and sensitive methods are highly required to detect CN À ion. Chemosensor application is one of the most useful methods for selective detection of the target analyte. BODIPY (boradiazaindacene) dyes are one of the promising candidates for these types of applications because of the unique optical and photophysical properties. Here, we report the first application of the meso-formyl-BODIPY dye-based, rapid and naked-eye CN À ion sensor application depending on the reaction of CN À ion and aldehyde functionality of the meso-formylated BODIPY derivative (BODIPY-BAL). Absorption and emission experiments were successfully conducted. Additionally, colorimetric tests were performed in solution and on test strips. The limit of detection of the chemosensor was calculated as 10.4 μM. Real sample experiments in seawater were also performed and CN À ion was detected at 50 μM concentration, which is the limit level of CN À ion concentration causes acute effects determined by the U.S. Environmental Protection Agency (EPA).

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Diagnosis of cyanide poisoning using an automated, field-portable sensor for rapid analysis of blood cyanide concentrations

Cited by 9 publications

References 21 publications

Endogenously produced hydrogen cyanide serves as a novel mammalian gasotransmitter

Endogenously produced hydrogen cyanide serves as a novel mammalian gasotransmitter

Photophysical and anion sensing properties of a triphenylamine–dioxaborinine trimeric compound

Rapid and Highly Selective BODIPY Based Turn‐Off Colorimetric Cyanide Sensor**

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