Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction

Miagostovich, Marize Pereira; Santos, Flávia Barreto dos; Araújo, Eliane Saraiva Machado de; Dias, Juarez Pereira; Schatzmayr, Hermann G.; Nogueira, Rita Maria Ribeiro

doi:10.1590/s0074-02761997000500006

Cited by 24 publications

(21 citation statements)

References 24 publications

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“…Recent improvements in research techniques could provide detection assays with increased sensitivity and improved accuracy. In agreement, recent publications have demonstrated the presence of dengue antigens in the CNS due to the detection of viral proteins, immunoglobulin, and RNA (Araujo et al , 2011; Lima et al , 2011; Miagostovich et al , 1997a; Miagostovich et al , 1997b). Viral infiltration and subsequent infection of the CNS have been linked directly to CNS viral replication.…”

Section: Immuno and Central Nervous System Pathogenesissupporting

confidence: 75%

Flaviviruses, an expanding threat in public health: focus on dengue, West Nile, and Japanese encephalitis virus

2014

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The flaviviruses Dengue, West Nile, and Japanese encephalitis represent three major mosquito-borne viruses worldwide. These pathogens impact the lives of millions of individuals and potentially could affect non-endemic areas already colonized by mosquito vectors. Unintentional transport of infected vectors (Aedes and Culex sp), traveling within endemic areas, rapid adaptation of the insects into new geographic locations, climate change, and lack of medical surveillance have greatly contributed to the increase in flaviviral infections worldwide. The mechanisms by which flaviviruses alter the immune and the central nervous system have only recently been examined despite the alarming number of infections, related deaths, and increasing global distribution. In this review, we will discuss the expansion of the geographic areas affected by flaviviruses, the potential threats to previously unaffected countries, the mechanisms of pathogenesis, and the potential therapeutic interventions to limit the devastating consequences of these viruses.

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Section: Immuno and Central Nervous System Pathogenesissupporting

confidence: 75%

Flaviviruses, an expanding threat in public health: focus on dengue, West Nile, and Japanese encephalitis virus

2014

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“…RT-PCR is a powerful tool in virological surveillance of DENV, especially when negative results are obtained by other tests such as virus isolation in cell culture [32]. Based on its high sensitivity, RT-PCR is used in epidemiological studies where large amounts of mosquitoes are collected.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Natural Vertical Transmission of Dengue-2 and Dengue-3 Viruses in Aedes aegypti and Aedes albopictus in Fortaleza, Ceará, Brazil

et al. 2012

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Background Aedes aegypti and Aedes albopictus perform an important role in the transmission of the dengue virus to human populations, particularly in the tropical and subtropical regions of the world. Despite a lack of understanding in relation to the maintenance of the dengue virus in nature during interepidemic periods, the vertical transmission of the dengue virus in populations of A. aegypti and A. albopictus appears to be of significance in relation to the urban scenario of Fortaleza. Methods From March 2007 to July 2009 collections of larvae and pupae of Aedes spp were carried out in 40 neighborhoods of Fortaleza. The collections yielded 3,417 (91%) A. aegypti mosquitoes and 336 (9%) A. albopictus mosquitoes. Only pools containing females, randomly chosen, were submitted to the following tests indirect immunofluorescence (virus isolation), RT-PCR/nested-PCR and nucleotide sequencing at the C-prM junction of the dengue virus genome. Results The tests on pool 34 (35 A. albopictus mosquitoes) revealed with presence of DENV-3, pool 35 (50 A. aegypti mosquitoes) was found to be infected with DENV-2, while pool 49 (41 A. albopictus mosquitoes) revealed the simultaneous presence of DENV-2 and DENV-3. Based on the results obtained, there was a minimum infection rate of 0.5 for A. aegypti and 9.4 for A. albopictus. The fragments of 192 bp and 152 bp related to DENV-3, obtained from pools 34 and 49, was registered in GenBank with the access codes HM130699 and JF261696, respectively. Conclusions This study recorded the first natural evidence of the vertical transmission of the dengue virus in populations of A. aegypti and A. albopictus collected in Fortaleza, Ceará State, Brazil, opening a discuss on the epidemiological significance of this mechanism of viral transmission in the local scenario, particularly with respect to the maintenance of these viruses in nature during interepidemic periods.

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“…RT-PCR from clinical samples identified DEN-1 and DEN-2 in 41% (41/ 100) of cases previously confirmed by serology where no virus isolation was achieved and proved to be an applicable method for retrospective dengue diagnosis, especially in fatal cases. In addition, it is rapidly becoming useful for monitoring dengue virus activity in new areas of the state (Miagostovich et al 1997b). Other RT-PCR protocols have also supported molecular epidemiological studies conducted over the years.…”

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confidence: 99%