Diagnosis of endometrial nerve fibers in women with endometriosis

Meibody, Fatemeh Aghaey; Kashi, Abolfazl Mehdizadeh; Mirzaie, Ali Zare; Amam, Marjan Ghajarie Bani; Behbahani, Afsane Shariati; Zolali, Bita; Najafi, Laily

doi:10.1007/s00404-010-1806-5

Cited by 25 publications

(12 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meticulous sampling technique for collection and proper orientation of the functional endometrial layer were methodologic points of emphasis. These results were confirmed in a study of 27 prospectively collected eutopic endometrial specimens using identical sampling and detection methods [ 142 ]. However, the adoption of this method in a clinical laboratory failed to recapitulate the accuracy of endometrial PGP9.5 immunohistochemistry in the diagnosis of women with endometriosis [ 143 ].…”

Section: Endometrial Biomarkersmentioning

confidence: 57%

Update on Biomarkers for the Detection of Endometriosis

Fassbender

Burney

et al. 2015

BioMed Research International

155

129

View full text Add to dashboard Cite

Endometriosis is histologically characterized by the displacement of endometrial tissue to extrauterine locations including the pelvic peritoneum, ovaries, and bowel. An important cause of infertility and pelvic pain, the individual and global socioeconomic burden of endometriosis is significant. Laparoscopy remains the gold standard for the diagnosis of the condition. However, the invasive nature of surgery, coupled with the lack of a laboratory biomarker for the disease, results in a mean latency of 7–11 years from onset of symptoms to definitive diagnosis. Unfortunately, the delay in diagnosis may have significant consequences in terms of disease progression. The discovery of a sufficiently sensitive and specific biomarker for the nonsurgical detection of endometriosis promises earlier diagnosis and prevention of deleterious sequelae and represents a clear research priority. In this review, we describe and discuss the current status of biomarkers of endometriosis in plasma, urine, and endometrium.

show abstract

Section: Endometrial Biomarkersmentioning

confidence: 57%

Update on Biomarkers for the Detection of Endometriosis

Fassbender

Burney

et al. 2015

BioMed Research International

155

129

View full text Add to dashboard Cite

show abstract

“…It is known that there are no nerve fibers in the functional layer of the endometrium. In recent years, however, many publications have identified nerve fibers in endometrial specimens of endometriosis patients who present with pain complaints 5 , 6 , 11 , 12 , 13 . Based on these findings, we thought that investigating the presence of nerve fibers in uterine leiomyoma patients could shed light on the etiopathogenesis of leiomyoma-associated pain.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Nerve Fiber Density between Patients with Uterine Leiomyoma with and without Pain: a Prospective Clinical Study

Giray

Esim-Buyukbayrak

Hallac-Keser

et al. 2018

Geburtshilfe Frauenheilkd

View full text Add to dashboard Cite

Introduction We aimed to compare the presence and the amount of nerve fibers in endometrial, myometrial and leiomyoma tissues using protein gene product 9.5 (PGP 9.5) and neurofilament (NF) immunohistochemical staining in uterine leiomyoma patients with and without pain complaint. Methods Patients undergoing hysterectomy for uterine leiomyoma were prospectively enrolled in the study. Twenty-five uterine leiomyoma patients without pelvic pain complaint (visual analog scale (VAS) < 5) were assigned to Group 1; 23 uterine leiomyoma patients with pelvic pain complaint (VAS ≥ 5) were assigned to Group 2. Endometrial, myometrial and leiomyoma tissues obtained from hysterectomy specimens were stained immunohistochemically using PGP 9.5 and NF dyes. The presence and density of nerve fibers were compared between the two groups. Results None of the endometrial samples in either groups stained with PGP 9.5 and NF dyes. There was no statistically significant difference in the number of nerve fibers in myometrial and leiomyoma tissues between the two groups with either of the stains (PGP 9.5: p = 0.39 and p = 0.29; NF: p = 0.83 and p = 0.65, respectively). There was agreement between PGP 9.5 and NF immunohistochemical staining for nerve fiber detection in myometrial and leiomyoma tissues (p < 0.05/κ = 0.622 and p < 0.05/κ = 0.388, respectively). Conclusion This study demonstrates that the quantity and density of nerve fibers in myometrial and leiomyoma tissue in patients with pain were similar to that in patients without pain.

show abstract

“…Similarly to us, Tokushige et al [21] using NF antibody did not detect these myelinated nerve fibres in these tissues, at difference to Bokor et al [12] who detected them in a few eutopic endometrial samples from endometriosis (5%) and control (10%) women. The neural protein usually detected in eutopic endometrium is PGP9.5 by a pan antibody, which recognizes both myelinated and non myelinated nerve fibres [8] [12]- [14] [42]. Both results suggest the lack of myelinated nerve fibres in the functional layer of eutopic endometrium.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of nerve fibres in endometriosis patients has been shown in eutopic (intrauterine) and ectopic endometria, including ovarian endometriomas, peritoneal endometriotic lesions and deep infiltrating endometriosis, but almost undetectable in control endometria from healthy women [9]- [11]. This important finding leads to the proposal to use nerve fibres detection as a diagnostic method through minimal invasive biopsy of eutopic endometria [12]- [14].…”

Section: Introductionmentioning

confidence: 97%

Nerve Fibres Detection in Paired Eutopic and Ectopic Endometria from Women with Endometriosis: Correlation with Nerve Growth Factor Expression

Venegas¹,

Parada²,

Boric³

et al. 2015

OJOG

View full text Add to dashboard Cite

Endometriosis, disorder characterized by the presence of endometrium outside the uterus cavity, is associated with chronic pelvic pain. Nerve growth factor (NGF) participates in development, repair and survival of neurons. We evaluated NGF and nerve fibres in eutopic and ectopic endometria from women with endometriosis and in normal endometrium from control patients without endometriosis. In endometrium collected during surgery for endometriosis (paired eutopic and ectopic endometria, proliferative n = 6 and secretory n = 7 phases) or during tubal ligation or hysterectomy for no endometrial disease (control, proliferative n = 6 and secretory n = 6 phases), NGF and neurofilament (NF) of nerve fibres were studied by immunohistochemistry measuring integrated optical density (IOD). Cytoplasmic NGF was detected in glands and stroma in all control, eutopic and ectopic endometrial samples; no statistical differences were found throughout the menstrual cycle. However, total (gland plus stroma) NGF IOD was higher in eutopic than in ectopic endometria during the secretory phase. NF was detected only in ectopic endometrium being eutopic and control endometria negative to this antibody, and no differences were observed between proliferative and secretory phases. Negative and significant correlation was found between NGF and NF expression in ectopic endometria only during the secretory phase. In conclusion, our results show a significant negative correlation between NGF and myelinated nerve fibres in secretory ectopic endometria. NGF expression was not significantly modified throughout the menstrual cycle in control and in endometriosis, even higher in eutopic than in ectopic tissues; the NGF secretion from lesions to pelvic cavity cannot be ruled out, and could explain the negative correla-S. Venegas et al. 418 tion observed. Although NF and NGF are not useful as diagnostic markers for endometriosis, their expression may be related to endometrial physiology and pathophysiology of the disease.

show abstract

Diagnosis of endometrial nerve fibers in women with endometriosis

Abstract: Our findings indicated that endometrial biopsy for detecting density of nerve fibers by usage of protein gene product 9.5, provided a reliable marker for diagnosis of endometriosis.

Cited by 25 publications

References 24 publications

Update on Biomarkers for the Detection of Endometriosis

Update on Biomarkers for the Detection of Endometriosis

Comparison of Nerve Fiber Density between Patients with Uterine Leiomyoma with and without Pain: a Prospective Clinical Study

Nerve Fibres Detection in Paired Eutopic and Ectopic Endometria from Women with Endometriosis: Correlation with Nerve Growth Factor Expression

Contact Info

Product

Resources

About