Diagnosis of extrapulmonary tuberculosis by polymerase chain reaction for mpb64 gene: An evaluation in a hospital based study

Pednekar, Sushma; Bhore, Arvind V.; Muley, Vrishali Avinash; Ghadage, Dnyaneshwari P.

doi:10.4103/0974-777x.112283

J Global Infect Dis

2013

DOI: 10.4103/0974-777x.112283

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Diagnosis of extrapulmonary tuberculosis by polymerase chain reaction for mpb64 gene: An evaluation in a hospital based study

Sushma Pednekar

Arvind V. Bhore

Vrishali Avinash Muley

et al.

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Polymerase Chain Reaction using the MPB64 fragment for detection of mycobacterium tuberculosis complex DNA in suspected TB cases

Aryal¹,

Sah²,

Paudel³

et al. 2014

Int J Med Sci Public Health

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Background: Various primers have been used for detection of Mycobacterium tuberculosis complex (MTBC) DNA using Polymerase chain reaction (PCR). The MPB64 gene has been demonstrated to be a highly specific target for MTBC detection. Aims & Objectives: To detect MTBC DNA using MPB64 primers in clinical specimens of Nepalese patients suspected to have tuberculosis (TB). Materials and Methods: DNA was extracted from 44 clinical specimens of patients suspected of having TB and MPB64 targeted PCR was performed. Results: Bands comprising 240 base pair (bp) of MPB64 sequence were present in 15 of the 44 clinical samples, of which 4 were pulmonary and the remainders were extra-pulmonary samples. The overall positivity of MPB64 targeted PCR was 34.1%. Conclusion: PCR targeting the MPB64 fragment has a potential of detecting the MTBC DNA and has potentially valuable clinical applications in early detection of TB in Nepal.

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Polymerase Chain Reaction using the MPB64 fragment for detection of mycobacterium tuberculosis complex DNA in suspected TB cases

Aryal¹,

Sah²,

Paudel³

et al. 2014

Int J Med Sci Public Health

View full text Add to dashboard Cite

show abstract

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Diagnosis of extrapulmonary tuberculosis by polymerase chain reaction for mpb64 gene: An evaluation in a hospital based study

Cited by 1 publication

References 0 publications

Polymerase Chain Reaction using the MPB64 fragment for detection of mycobacterium tuberculosis complex DNA in suspected TB cases

Polymerase Chain Reaction using the MPB64 fragment for detection of mycobacterium tuberculosis complex DNA in suspected TB cases

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