Diagnosis of Helicobacter pylori infection with the ¹³C‐urea breath test by means of GC‐MS analysis

Abstract

The diagnosis of Helicobacter pylori (H. pylori) infection by GC-MS detection of the (13)CO(2) enrichment in (13)C-urea breath test ((13)C-UBT) samples is reported. This study aimed to optimize the (13)C-UBT with regards to the diagnostic cut-off value, sampling time, and frequency. The H. pylori status of 103 dyspeptic patients was obtained by histological examination, the rapid urease test as well as with the GC-MS (13)C-UBT. Analytical and diagnostic accuracies were determined by comparison of the GC-MS (13)C-UBT results with that of the analytical and diagnostic gold standards, namely GC-isotope ratio MS (IRMS) and histology. The (13)CO(2) enrichment values obtained with GC-MS analysis, correlated favorably (r(2) = 0.993) with those obtained by GC-IRMS analysis. When compared to histology, the GC-MS (13)C-UBT had a diagnostic sensitivity of 92% and a specificity of 93%. The positive predictive value (PPV), negative predictive value (NPV), and accuracy were 95, 89, and 92%, respectively. It was concluded that SIM GC-MS is capable of analyzing nonradioactive (13)C-UBT samples, with a precision and accuracy sufficient to distinguish between H. pylori positive and negative patients.

Diagnosis of Helicobacter pylori

“…A simplified 13 C‐UBT protocol with no test meal gave the best results at 100% for all performance parameters. A study from South Africa [30] compared the simpler and cheaper GC‐MS to the standard IRMS and found a sensitivity and specificity of 92% and 93%, respectively. Another simplified 13 C‐UBT showed a sensitivity and specificity of 90.2% and 93.3%, respectively [31].…”

Residual gas analyzer mass spectrometry for human breath analysis: a new tool for the non-invasive diagnosis of Helicobacter pylori infection

“…Interestingly, the OA-IOCS measurement also demonstrated a significant higher value of δ 13 DOB C‰ = 4.8‰ of that individual compared to the cutoff value of 3.0‰. The reason for producing false positive results in the endoscopy-based biopsy test is still a subject of controversy and subsequently several possible causes have been reported [4,20]. It is known that H. pylori has a very patchy distribution throughout the stomach.…”

Mechanism of lignin degradation via white rot fungi explored using spectral analysis and gas chromatography-mass spectrometry

“…The GC-MS analysis was performed as previously described by Jordaan and Laurens (2015). Samples from the degradation system were collected after running the reaction for 24 h, and the samples were analyzed via GC-MS.…”