Diagnosis ofHelicobacter pyloriInfection in Children: Comparison of a Salivary Immunoglobulin G Antibody Test with the [13C]Urea Breath Test

Bode, Günter; Marchildon, P; Peacock, Jeffrey; Brenner, Hermann; Rothenbacher, Dietrich

doi:10.1128/cdli.9.2.493-495.2002

Cited by 20 publications

(20 citation statements)

References 26 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Six papers evaluated the presence of antibodies in oral specimens using a variety of commercially available kits and collection devices [3,5,38,41,77,78,107]. At least two of the studies sampled asymptomatic preschool children (in day-care or random samples) and used UBT as the reference standard [77,78].…”

Section: Serologymentioning

confidence: 99%

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

et al. 2009

View full text Add to dashboard Cite

The array of tests that can be used for diagnosis of Helicobacter pylori infection is large, and it can be confusing to define which test to use particularly in children where results may not be comparable to those obtained in adult patients. Using PubMed, we reviewed the English literature from January 1999 to May 2009 to identify articles that determined sensitivity and specificity of H. pylori invasive and non-invasive diagnostic tests in children. We excluded articles that presented a review of the literature, abstracts, case reports, or series where children's results could not be separated from adult populations. Of the tissue based methods, rapid urease tests have better sensitivity than histology to detect presence of H. pylori; however, histology can detect the pathology associated with disease including gastritis, intestinal metaplasia, and other conditions that could be the cause of the child's symptoms. Culture of gastric tissues or stool has 100% specificity but sensitivity is low. Of the serologic tests, immunoblot has the best sensitivity. The urea breath tests have >75% sensitivity for detection of H. pylori before and after treatment. Immunoassays in stool using monoclonal antibodies have >95% sensitivity for detection of H. pylori before and after treatment. PCR testing can be performed in tissue and stool samples and can detect genes associated to antibiotic resistance. In summary, the current commercial non-invasive tests have adequate sensitivity and specificity for detecting the presence of H. pylori; however, endoscopy with histopathology is the only method that can detect H. pylori and lesions associated with the infection.

show abstract

Section: Serologymentioning

confidence: 99%

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

et al. 2009

View full text Add to dashboard Cite

show abstract

“…H. pylori stool antigen; HpSA test), have been developed and efficiently used in diagnosis of H. pylori infection (20). It was reported that noninvasive tests such as 13C-UBT, H. pylori stool antigen and anti-H. pylori immunoglobulin G antibody are relatively concordant and specific for the diagnosis of H. pylori infection (21,22). Furthermore, UBT has excellent sensitivity and specificity in lab diagnosis of H. pylori as mentioned previously (12,23).…”

Section: Discussionmentioning

confidence: 97%

Potential Complications of Helicobacter pylori Infection in Children of a Non-Urban Community

Ali

Elkhatib

2015

Arch Pediatr Infect Dis

View full text Add to dashboard Cite

Background:Helicobacter pylori (H. pylori) is one of the most common chronic bacterial infections world-wide and has several adverse consequences particularly on health of pediatric patients. Objectives: The current study addressed various clinical presentations associated with H. pylori infections among children of a rural community. Materials and Methods:In this case-control study, the number of cases (H. pylori-positive) were 120 (52.5% boys and 47.5% girls) and the control group (H. pylori-negative) were 100 cases aged 7 -12 years old (Mean 9.9 ± 3 years). H. pylori infection was defined by positive results for H. pylori stool antigen test and serum IgG or urea breath test. All pediatric participants were assessed for weight, height, sideropenic anemia and gastrointestinal symptoms. Results:The results showed that recurrent abdominal pain, recurrent vomiting and anorexia were 81 (67.5%), 24 (20%) and 15 (12.5%), respectively in H. pylori-infected patients, compared to 28 (28%), 8 (8%) and 4 (4%) in control group. Weight and height were 20.4 ± 1 kg and 128.1 ± 1 cm in case group compared to 25.6 ± 1.7 kg and 133.8 ± 2 cm in control group. Sideropenic anemia was 36.7% in patients compared to 15% in control. Conclusions: In conclusion, the study findings revealed that pediatric H. pylori infection is associated with recurrent abdominal pain, anorexia and recurrent vomiting, also adversely affects the growth of children in various modalities; in particular weight, height and progressive incidence of sideropenic anemia.

show abstract

“…Meta-analysis searching indicated that H. pylori gastric infection doubles the likelihood of finding H. pylori in the mouth [14]. This searching was based on oral H. pylori identification by PCR, culture, urease activity, or salivary antibodies [6,9,10,11,15]. H. pylori has also been detected in the oral microbiome by DNA sequencing [16].…”

Section: Discussionmentioning

confidence: 99%

“…However, oral screening is not routinely available, despite evidence as early as 1989 for H. pylori in the oral cavity [8]. Studies of human dental plaque and saliva have identified H. pylori by PCR, culture, urease activity, and specific oral antibodies [7,9,10,11]. But the application of these techniques to H. pylori detection has been variable and sometimes controversial.…”

Section: Introductionmentioning

confidence: 99%

A Screening Trial of Helicobacter pylori-Specific Antigen Tests in Saliva to Identify an Oral Infection

Yee¹,

Wei²,

Yee³

et al. 2013

Digestion

View full text Add to dashboard Cite

Objective:Helicobacter pylori infection places a heavy burden on medical and economic resources. Standard diagnosis requires the presence of established H. pylori gastric disease. Study Design and Setting: A multicenter screening trial assessing 2 immunochromatographic H. pylori antigen oral tests was carried out with 201 participants. The analysis also included a urea breath test (UBT), a Campylobacter-like organism test, silver stain, culture, serology, and stool tests. Results: The participants were grouped into UBT positive (UBT+) and UBT negative (UBT-) people, using conventional methods with congruent clusters based on p values from McNemar's paired χ² analysis and 95% CI estimates. Both oral tests were also positive in 82% of the seropositive UBT- people. However, oral antigen and seroprevalence divided UBT- people into 2 statistically separate CI subgroups: the UBT- symptomatic (highly positive) group and the UBT- asymptomatic (mostly negative) group. 90.5% of all people whose oral tests were both negative were also UBT-. Conclusions: Saliva H. pylori antigen is an important indicator in UBT- asymptomatic patients. Currently, its clinical significance remains uncertain, but saliva may be a reservoir from where H. pylori is transmitted to the stomach. In symptomatic patients, it is strongly associated with stomach infection.

show abstract

Diagnosis ofHelicobacter pyloriInfection in Children: Comparison of a Salivary Immunoglobulin G Antibody Test with the [¹³C]Urea Breath Test

Cited by 20 publications

References 26 publications

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

Potential Complications of Helicobacter pylori Infection in Children of a Non-Urban Community

A Screening Trial of <b><i>Helicobacter pylori</i></b>-Specific Antigen Tests in Saliva to Identify an Oral Infection

Contact Info

Product

Resources

About