Diagnosis of Liver Fibrosis With Wisteria floribunda Agglutinin-Positive Mac-2 Binding Protein (WFA-M2BP) Among Chronic Hepatitis B Patients

Abstract

BackgroundWisteria floribunda agglutinin-positive Mac-2 binding protein (WFA-M2BP) is a protein with altered glycosylation that reacts with lectin, and was recently identified as a useful non-invasive biomarker for the diagnosis of liver fibrosis in patients with hepatitis C virus infection.This study aimed to evaluate the diagnostic efficacy of WFA-M2BP for liver fibrosis in the context of hepatitis B virus (HBV).MethodsWe enrolled 151 patients infected with HBV. Liver biopsy and elastography (Fibroscan) were performed during the initial visit. Fibrosis was graded according to the Knodell histologic activity index (F0–3). WFA-M2BP levels were determined with an automated immunoassay analyzer (M2BPGi, HISCL-5000, Sysmex, Japan). The diagnostic efficacy of WFA-M2BP was compared with those of various conventional or composite biomarkers, including enhanced liver fibrosis (ELF) score, Fibroscan, aspartate transaminase (AST)-to-platelet ratio index (APRI), and FIB-4, based on the area under the ROC curve (AUC) value.ResultsThe majority of patients were at fibrosis stages F1 and F2. The F2 and F3 AUC values for WFA-M2BP were similar to those for FIB-4, APRI, ELF, and Fibroscan, although the latter showed the best diagnostic efficacy. The diagnostic accuracy of all tested biomarkers for F2 and F3 was 60–70%. In multivariate analysis, WFA-M2BP, ELF, and platelet count significantly predicted stage ≥F2, whereas only platelet count significantly predicted F3.ConclusionsWFA-M2BP can support a diagnosis of liver fibrosis with similar diagnostic efficacy to other biomarkers, and predicted liver fibrosis stage ≥2 among patients with chronic hepatitis B.

Fibrosis Staging Using Direct Serum Biomarkers is Influenced by Hepatitis Activity Grading in Hepatitis C Virus Infection

“…WFA-M2BP and ELF score are comparable in the diagnosis of advanced liver fibrosis as shown by Mann–Whitney U test in stage-by-stage analysis, ROC curve analysis, sensitivity, and specificity. The results followed the comparison of these two biomarkers based on fibrosis staging by transient elastography in a cohort comprised mainly of HBV-infected patients [ 27 ]. Contrary to recent work, neither WFA-M2BP nor ELF score were able to distinguish stage 2 from stage 1 in the current cohort, which might be due to the relatively smaller number of patients in the fibrosis stage 1 group 19.…”

Procalcitonin as a prognostic marker for sepsis based on SEPSIS‐3

“…Hepatitis C viral infection was excluded if anti‐HCV was negative. Respiratory virus infection was excluded if the interpretation of radiologic study was negative or 14 kinds of multiplex virus PCR (influenza A, influenza B, parainfluenza 1,2,3, respiratory syncytial virus A, B, adenovirus, metapneumovirus, rhinovirus, three kinds of coronavirus and bocavirus) were negative . Demographic data and baseline characteristics of patients were collected at the time of admission.…”

Performance evaluation of serum PIVKA‐II measurement using HISCL‐5000 and a method comparison of HISCL‐5000, LUMIPULSE G1200, and ARCHITECT i2000

“…It is important to detect early‐stage HCC in high‐risk groups such as those with HBV infection, HCV infection, or cirrhosis. Several serum markers for diagnosing liver fibrosis or cirrhosis are known, and the diagnosis of HCC is based on pathology or imaging studies . Regular surveillance is warranted in these high‐risk groups .…”

“…Several serum markers for diagnosing liver fibrosis or cirrhosis are known, and the diagnosis of HCC is based on pathology or imaging studies. 2,3 Regular surveillance is warranted in these high-risk groups. 2,4,5 HCC surveillance programs for high-risk groups have been performed using ultrasound and serum alpha-fetoprotein (AFP) level in Korea.…”