Diagnosis of mycobacterial drug resistance in HIV reactive patients by phenotypic and genotypic assay - a comparative study

Umamaheshwari, S.; Neelambike, Sumana M.; Anuradha, K.; Sumangala, B

doi:10.1186/1471-2334-14-s3-p58

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“…MDR of the three groups were 5 (6.6%), 2 (6.7%), and 4 (8%), respectively [ Table 4 ]. [ 8 ] The MDR rate detected in these groups were statistically (Chi-square test) found to be insignificant.…”

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confidence: 99%

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Drug resistance pattern of mycobacterial isolates in HIV and non-HIV population in South India

Shivaswamy

Neelambike

2016

Lung India

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Background:Emergence of drug resistance has complicated the treatment of tuberculosis (TB). WHO reports India to be one among 27 “high burden” multidrug-resistant (MDR) TB countries.Objective:To diagnose TB and detect drug resistance of mycobacterial isolates in acid-fast bacilli (AFB) smear negative HIV reactive patients (Group A) and compare them with HIV seropositive AFB smear positive (Group B) and HIV-seronegative AFB positive cases (Group C).Materials and Methods:Clinical specimens collected in all groups were processed as per the standard protocol except blood, which was processed by lysis centrifugation technique. They were then inoculated with Lowenstein-Jensen media and the isolates obtained were subjected to drug susceptibility test (DST) by proportion method and genotype MTBDR plus assay.Results:In Group A, 162 patients were included. Of the 443 clinical samples collected, 76 mycobacterial strains were obtained from 67 (41%) patients. Of these, 50 (65.8%) were sensitive to all drugs and 26 (34.2%) resistant to one or more anti-tubercular drugs. Antibiogram of Group A when compared with Group B and C showed that the MDR rate 6.6%, 6.7% and 8% respectively) did not differ much; but resistance to at least single drug was (26 [34.2%], 3 [10%], and 8 [16%]), respectively.Conclusion:Our study suggests that HIV has no influence on the anti-tubercular resistance pattern, but increased MDR rate along with HIV in high TB burden setting stresses the need for early diagnosis and DST in providing proper regimens and improve prognosis.

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confidence: 99%

“…All the results of the conventional proportion method concurred with the molecular genotype MTBDR plus assay except one isolate which showed resistance to RIF solely by the latter method. [ 8 ]…”

Section: Resultsmentioning

confidence: 99%

Drug resistance pattern of mycobacterial isolates in HIV and non-HIV population in South India

Shivaswamy

Neelambike

2016

Lung India

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Diagnosis of mycobacterial drug resistance in HIV reactive patients by phenotypic and genotypic assay - a comparative study

Abstract: Umamaheshwari et al.: Diagnosis of mycobacterial drug resistance in HIV reactive patients by phenotypic and genotypic assay-a comparative study. BMC Infectious Diseases 2014 14(Suppl 3):P58.

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Drug resistance pattern of mycobacterial isolates in HIV and non-HIV population in South India

Drug resistance pattern of mycobacterial isolates in HIV and non-HIV population in South India

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