Diagnosis of mycoplasma pneumoniae by loop-mediated isothermal amplification: systematic review and meta-analysis

Abstract

Background A novel method, termed loop-mediated isothermal amplification (LAMP), was developed by Notomi et al. (2000). Individually published results have been reported that this technology has been successfully applied to the detection of a variety of pathogens. However, the overall diagnostic accuracy of LAMP for Mycoplasma pneumoniae (MP) remains unclear. A meta-analysis was therefore performed to review the accuracy of LAMP for Mycoplasma pneumoniae. Methods Cochrane Library and PubMed were systematically searched and checked for studies using LAMP for detecting mycoplasma pneumoniae. We used PCR as a reference standard to evaluate the quality of the studies eligible for inclusion in the meta-analysis. Then, the data from the studies were extracted by two independent assessors. Meta-DiSc 1.4 software was utilized to test the heterogeneity of sensitivity (SEN), specificity (SP), positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnosis odds ratio (DOR). The pooled analysis results were plotted, and the summary receiver operating characteristic (SROC) curve was plotted by calculating the area under the curve (AUC). Generated pooled summary estimates (95% CIs) were calculated for the overall accuracy, and a bivariate meta-regression model was used for the meta-analysis. Results Seven studies with nine fourfold tables were included in this meta-analysis. The pooled SEN and SPE for diagnosing Mycoplasma pneumoniae were 0.90 (95% CI: 0.87–0.93) and 0.98 (95% CI: 0.96–0.99), respectively. The PLR was 31.25 (95% CI: 14.83–65.87), NLR 0.10 (95% CI: 0.05–0.22), DOR 399.32 (95% CI: 172.01–927.00), and AUC 0.9892. Conclusions In conclusion, compared with PCR, LAMP is a valuable alternative method for Mycoplasma pneumoniae diagnosis in clinic with high sensitivity and specificity. However, more evidence is required to confirm that LAMP can fully replace other methods in the clinical diagnosis of MP.

Diagnostic value of loop‐mediated isothermal amplification assay for hand, foot, and mouth disease

“…12 It has a widespread application in the diagnosis of infectious diseases such as malaria and trypanosomiasis. 13 Moreover, it has been regarded as a potential alternative to PCR in the laboratory. 14 However, the diagnostic accuracy of LAMP for HFMD diagnosis remains unclear.…”

Pooled analysis of LAMP assay for the diagnosis of norovirus infection

“…The most significant one is its rapidity; LAMP allows immediate diagnosis in just 30–50 min 36 . Recently, LAMP has been utilized for the rapid detection of some pathogens, and their results showed high accuracy and availability in the diagnosis of the corresponding pathogens 37‐39 . In the detection of norovirus, LAMP with hydroxynaphthol blue dye could be observed visually through the change of color; therefore, the instrument of molecular electrophoresis is not required 27,28,31 …”

Gram-Positive Pneumonia: Possibilities Offered by Phage Therapy

“…This method can be applied for the detection of both viruses and bacteria, since both DNA and RNA can be amplified. So far, this technique has proven to be useful for the detection of M. pneumoniae [ 26 ] and S. pneumoniae [ 27 ], among others.…”