Diagnosis of Pulmonary and Extra Pulmonary Tuberculosis: How Best is CBNAAT when Compared to Conventional Methods of TB Detection?

Kandi, Subhakar; Reddy, Vikasana; Nagaraja, Sharath Burugina

doi:10.17140/prrmoj-4-137

Cited by 12 publications

(5 citation statements)

References 2 publications

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“…For extra-pulmonary samples ,the sensitivity and specificity of CBNAAT samples were 85.7% and 93.5% respectively while that for smear negative were 60.7% and 100% respectively. 16 CBNAAT is a useful surrogate test for screening for MDR-TB as past studies on drug resistance have shown that rifampicin resistance seldom occurs alone and around 90% of rifampicin resistant patients are diagnosed to have MDR -TB. 12 The specificity of CBNAAT in detecting rifampicin resistance is very high (98%), and increasing evidence has shown that the infrequent occurrence of so-called false-positive results may be linked to the detection by Xpert MTB/RIF of strains that are truly resistant to rifampicin, but which are not detected by the phenotypic culture-based DST, the present reference standard.…”

Section: Resultsmentioning

confidence: 99%

Pattern of tuberculosis among tribal population of Central India with special reference to cartridge based nucleic acid amplification test as diagnostic tool: a descriptive study at tertiary care hospital

2019

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Background: Tuberculosis (TB) kills close to half a million Indians every year. Lack of reliable rapid diagnostic techniques for TB hampers timely diagnosis and leads to continued disease transmission, causing significant morbidity and mortality. The potential of newly recommended CBNAAT in TB and MDR-TB detection has been underutilized in our area due to lack of awareness regarding the same. Hence we utilized this rapid, logistically simplified test to study the pattern of tuberculosis among tribal population of Central India.Methods: Descriptive study of suspected TB patients in tertiary care centre from March 2016 to March 2019. Appropriate specimens from suspected TB patients were collected and subjected to CBNAAT and AFB smear to study the pattern of TB and Rifampicin- Resistant(RR) TB in our area.Results: CBNAAT detected overall 27% MTB cases; 27.72 % Pulmonary-TB cases as against smear positivity rate of 20.73% whereas 12.74% Extra-pulmonary-TB (EPTB) cases as against smear positivity rate of 1.59%.Overall 94.91% were RiF Sensitive( RS-TB) and 4.58% were RR-TB. Of the 57 (4.16%) HIV-TB coinfected cases; 96.49% were RS-TB and 5.26% were RR-TB. Co-infected patients have high incidence of EPTB(21.05%) involvement with RR-TB 3.50%. Among EPTB cases; lymph node aspirate and pus provided highest CBNAAT positive cases and almost 90.62% EPTB specimens were RS-TB .Conclusions: Availability of new diagnostic services has increased early identification of TB and RR-TB. Awareness among physicians regarding diagnostic utility of CBNAAT should be further increased as early identification of possible MDR cases is key to reducing community transmission and treatment initiation, particularly in high-burden, resource-limited settings.

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Section: Resultsmentioning

confidence: 99%

Pattern of tuberculosis among tribal population of Central India with special reference to cartridge based nucleic acid amplification test as diagnostic tool: a descriptive study at tertiary care hospital

2019

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“…CB-NAAT as a rapid diagnostic test, should be routinely used in public and private health sectors efficiently for the detection of a case of tuberculosis. 11 In cases of extrapulmonary tuberculosis, nucleic acid amplification tests appears to be an attractive modality with a high specificity, positive predictive value and highly variable sensitivity. 12…”

Section: Discussionmentioning

confidence: 99%

Spectrum of diseases presenting with lymphadenopathy in elderly: Fine needle aspiration cytology of 60 cases

Kushtagi¹,

Husain²,

.R³

2020

ACHR

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Introduction: Lymphadenopathy is one of the common clinical presentations in patients of all age group. Lymphadenopathy can be due to reactive or infective cause, but malignancy including metastastic deposits in are not uncommon in elderly. Fine needle aspiration cytology (FNAC) is a useful diagnostic rapider in many mass lesions like lymphadenopathy. Aims and Objectives: Three years study was conducted to know the spectrum of diseases causing enlargement of lymph nodes in elderly patients with age 60 years and above. Materials and Methods:We have collected all the clinical data from the registers from June 2014 to June 2017 in Medical records section, KIMS, Koppal and reviewed FNAC slides of lymph node aspirates of elderly. Results: A total of 60 elderly patients of age 60 years and above with lymphadenopathy were included in this study. Cervical group of lymph nodes were the predominant site for lymphadenopathy. The commonest cause of lymphadenopathy was reactive, seen in 29 patients, followed by 23 patients with metastatic deposits and squamous cell differentiation was commonest type. Tubercular and suppurative lymphadenitis were observed in four cases each. CBNNAT is an useful diagnostic modality in microbiological confirmation of tuberculosis. Males showed preponderance of reactive as well as in metastatic lymphadenopathy. Conclusion: Though reactive lymphadenitis is the commonest cause for enlargement of lymph nodes but metastatic deposits are not uncommon in elderly. FNAC as a diagnostic rapider useful screening tool for all elderly patients presenting with lymphadenopathy.

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“…In a study done Kandi et al the sensitivity of CBNAAT for pulmonary samples was 79% when compared to sputum smear which was 42%. [6][7][8] Cite this article as: Kumar P, Bhardwaj P. Diagnosis of pulmonary tuberculosis with cartridge based nucleic acid amplification test and light emitting diode fluorescent microscopy: a comparative study. Int J Adv Med 2019;6:1580-3.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of pulmonary tuberculosis with cartridge based nucleic acid amplification test and light emitting diode fluorescent microscopy: a comparative study

Kumar

Bhardwaj

2019

Int J Adv Med

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Background: Due to low sensitivity and inability to detect drug resistance, smear microscopy limits its impact on TB control. Culture methods and drug susceptibility testing is complex, time consuming, and takes around 6-8 weeks. A new diagnostic test, cartridge based nucleic acid amplification test (CBNAAT) was developed based on real-time polymerase chain reaction (RT PCR). Objective of this study to compare the results of CBNAAT for diagnosis of pulmonary tuberculosis with LED fluorescent microscopy and sputum culture.Methods: A cross-sectional study was conducted in the department of Chest and TB, CIMS, Bilaspur. Each Sputum sample of presumptive TB patients were tested with CBNAAT, sputum smear microscopy by light emitting diode (LED) fluorescent microscopy (FM) and solid and liquid culture for diagnosis of Tuberculosis. Results of CBNAAT, Fluorescent Microscopy and Culture for detection of Mycobacterium Tuberculosis were compared.Results: The sensitivity and specificity for CBNAAT were 97% and 100% respectively; while that for Fluorescent microscopy were 70% and 100% respectively. The positive and negative predictive value for CBNAAT was 100% and 96% respectively. The positive and negative predictive value for Fluorescent microscopy was 100% and 73% respectively.Conclusion: CBNAAT is having high sensitivity and specificity for diagnosis of pulmonary tuberculosis. It should be routinely used under national health programme to detect a tuberculosis case efficiently.

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Diagnosis of Pulmonary and Extra Pulmonary Tuberculosis: How Best is CBNAAT when Compared to Conventional Methods of TB Detection?

Cited by 12 publications

References 2 publications

Pattern of tuberculosis among tribal population of Central India with special reference to cartridge based nucleic acid amplification test as diagnostic tool: a descriptive study at tertiary care hospital

Pattern of tuberculosis among tribal population of Central India with special reference to cartridge based nucleic acid amplification test as diagnostic tool: a descriptive study at tertiary care hospital

Spectrum of diseases presenting with lymphadenopathy in elderly: Fine needle aspiration cytology of 60 cases

Diagnosis of pulmonary tuberculosis with cartridge based nucleic acid amplification test and light emitting diode fluorescent microscopy: a comparative study

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