Diagnosis of pulmonary tuberculosis using Ziehl–Neelsen stain or cold staining techniques?

Abdelaziz, Maha M.; Bakr, Wafaa; Hussien, Somya M.; Amine, Amira

doi:10.1097/01.epx.0000481358.12903.af

Cited by 18 publications

(9 citation statements)

References 14 publications

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“…Comparison of ZN staining to the culture, which is regarded as gold standard, in this study showed higher sensitivity and slightly less specifi city than previous study (Abdelaziz et al 2016). The study conducted…”

Section: Discussionmentioning

confidence: 58%

Drug Susceptibility Profile of Mycobacterium tuberculosis Isolated from Patients Visiting National Tuberculosis Centre, Nepal

et al. 2018

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Objectives: The objective of this study was to assess drug susceptibility pattern of Mycobacterium tuberculosis (MTB). Methods: This cross-sectional study was carried out among 145 clinically suspected and previously treated pulmonary tuberculosis patients visiting National Tuberculosis Centre, Bhaktapur, Nepal. After obtaining written informed consent, questionnaire was administered and sputum samples were collected from each patient. Each sample was subjected to Ziehl-Neelsen (ZN) staining and cultured on Lowenstein Jensen (LJ) medium at 37ºC for 8 weeks. MTB isolates were identified by growth rate and colony morphology, confirmed by biochemical tests and drug susceptibility testing (DST) of identified isolates was performed by proportion method. Results: A total of 49.7% (n=72) sputum samples were positive for MTB by culture and 46.9% (n=68) were positive by ZN staining. Among culture positive isolates of MTB (n= 72), 25% (n=18) were resistant to at least one drug. The prevalence of multi drug resistant tuberculosis (MDR-TB) was 15.3% (n=11) of which 5.56% (n=4) were resistant to rifampicin (RIF) only, 1.39% (n= 1) were resistant to isoniazid (INH) only. Out of 18 resistant isolates, 61.1% (n=11) were resistant to both RIF and INH, 21.43% (n=3) resistant to INH were susceptible to RIF and 26.67% (n=4) resistant to RIF were susceptible to INH. Smoking (P=0.001) and coughing (P=0.009) were statistically significant with isolation of MTB. Conclusion: Since the prevalence of MDR-TB was high, MDR-TB strains should be identified in order to initiate second line treatment.

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Section: Discussionmentioning

confidence: 58%

Drug Susceptibility Profile of Mycobacterium tuberculosis Isolated from Patients Visiting National Tuberculosis Centre, Nepal

et al. 2018

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“…there is a significant relationship between smoking habit and the incidence of tuberculosis and people who smoke for a period of > 10 years have a 2 times greater risk of developing tuberculosis, through interviews conducted by respondents who have a smoking habit and suffer from tuberculosis claiming that they cannot stop smoking because they are addicted Even though they already know the dangers of smoking, the smoking behavior of people with tuberculosis has been going on for years and they still don't stop smoking even though they have tested positive for tuberculosis, smoking with the incidence of tuberculosis is a double problem because smoking helps spread the infection and worsens the severity of tuberculosis. [19]. [20].…”

Section: Resultsmentioning

confidence: 99%

Identification of Mycobacterium Tuberculosis in Active Smokers with Ziehl–Neelsen Staining Method

2022

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Tuberculosis is still a health problem for the people of Indonesia, especially active smokers. There are many risk factors that can cause tuberculosis in active smokers including age at starting smoking, number of cigarettes consumed per day, and how long smoking has been done. The purpose of this study was to determine the results of identifying mycobacterium tuberculosis in active smokers with the Ziehl–Neelsen staining acid resistance test method. This type of research is a descriptive-analytic study that aims to determine the results of identifying Mycobacterium tuberculosis in active smokers. The Ziehl–Neelsen staining acid resistance test was conducted at the Tourism Hospital Laboratory of the University of East Indonesia Makassar on September 22 to October 6, 2022. The population in the study is 10 people. The sample was determined using a total sampling technique so that 10 samples were obtained. The research variables were active smokers. The data obtained are presented in the form of tables and narratives. After laboratory testing using the Ziehl–Neelsen staining method, the conclusion was that 1 out of 10 samples of active smokers was identified as Mycobacterium Tuberculosis.

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“…In addition to infectious etiologies identified by culture, microscopy identified seven patients with acid-fast bacilli (AFB) present in sputum smears. Because the presence of AFB in sputum is considered indicative of Mycobacterium tuberculosis infection, these patients were treated as tuberculosis (TB) patients [ 40 ]. Microscopy also identified Plasmodium species in three patients.…”

Section: Resultsmentioning

confidence: 99%

An Observational Study of Sepsis in Takeo Province Cambodia: An in-depth examination of pathogens causing severe infections

et al. 2020

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The world’s most consequential pathogens occur in regions with the fewest diagnostic resources, leaving the true burden of these diseases largely under-represented. During a prospective observational study of sepsis in Takeo Province Cambodia, we enrolled 200 patients over an 18-month period. By coupling traditional diagnostic methods such as culture, serology, and PCR to Next Generation Sequencing (NGS) and advanced statistical analyses, we successfully identified a pathogenic cause in 46.5% of our cohort. In all, we detected 25 infectious agents in 93 patients, including severe threat pathogens such as Burkholderia pseudomallei and viral pathogens such as Dengue virus. Approximately half of our cohort remained undiagnosed; however, an independent panel of clinical adjudicators determined that 81% of those patients had infectious causes of their hospitalization, further underscoring the difficulty of diagnosing severe infections in resource-limited settings. We garnered greater insight as to the clinical features of severe infection in Cambodia through analysis of a robust set of clinical data.

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Diagnosis of pulmonary tuberculosis using Ziehl–Neelsen stain or cold staining techniques?

Abstract: Gabbett's stain was more accurate in the diagnosis of PTB compared with both ZN and MCS, even though the results were not statistically significant. It can replace ZN for initial diagnosis and follow up of cases. It is also easier, faster, and does not require a heating step.

Cited by 18 publications

References 14 publications

Drug Susceptibility Profile of Mycobacterium tuberculosis Isolated from Patients Visiting National Tuberculosis Centre, Nepal

Drug Susceptibility Profile of Mycobacterium tuberculosis Isolated from Patients Visiting National Tuberculosis Centre, Nepal

Identification of Mycobacterium Tuberculosis in Active Smokers with Ziehl–Neelsen Staining Method

An Observational Study of Sepsis in Takeo Province Cambodia: An in-depth examination of pathogens causing severe infections

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