Diagnosis of Quinine Hypersensitivity, Use of Platelet Factor 3 and Acid Phosphatase Availability Tests

Abstract

Two patients with a known quinine induced allergic thrombocytopenia have been studied in order to detect in vitro the presence of drug-dependent antibodies in their plasma. The platelet factor 3 and platelet acid phosphatase availability tests have shown that these antibodies are still present several years after the last exposition to quinine followed by the typical clinical symptoms. The demasking of acid phosphatase activity is increased in presence of calcium ions. The possible mechanisms leading to the demasking of PF 3 and acid phosphatase after exposition of the patient’s platelets to quinine are discussed

Lysosomal Consept of Platelet Secretion ‐ Revisited

“…While true release or secretion of acid phosphatase activity into serum was observed only during platelet rich plasma clotting and retraction (22, 49), majority of acid phosphatase activity in stimulated platelets is sedimentable with platelet aggregates (50)(51)(52)(53). A possibility that pnitrophenylphosphate substrate measures true availability of acid phosphatase activity originating from platelet storage organelles (22,24,(50)(51)(52)(53)(107)(108)(109) seems to be supported by the timing of acridin orange release from platelet granules in a recalcified platelet rich plasma. Both events occurring only after a formation of a fibrin meshwork (51,107,256).…”

Procoagulant potential of platelet α granules

“…35,36 Scott syndrome platelets are characterized by a lack of PS availability and MV formation after activation. 4,37 It seems fair to predict that they will also show lack of acid phosphatase availability upon activation.…”