Diagnosis of systemic mycoses by specific immunohistochemical tests

Jensen, Henrik Elvang; Schønheyder, Henrik Carl; Hotchi, Masao; Kaufman, Leo

doi:10.1111/j.1699-0463.1996.tb00714.x

Cited by 73 publications

(80 citation statements)

References 71 publications

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“…Situação semelhante tem sido descrita por outros autores em infecções micóticas que, muitas vezes, não apresentam alterações clínicas características que permitam um diagnóstico presuntivo (Kaufman 1992, Jensen et al 1996, seguido da ausência de cultura micológica, que é a técnica padrão-ouro (Lima et al 1977), e que permite a identificação definitiva do agente etiológico. Consequentemente, o agente causal de doenças micóticas (e oomicóticas) pode ser encontrado ou suspeitado somente durante o exame histopatológico dos tecidos afetados (Jensen et al 1996). Nesses casos, nos quais não é possível a cultura e a identificação do fungo, o reconhecimento das características morfológicas e histoquímicas das leveduras de Cryptococcus spp.…”

Section: Discussionunclassified

“…Infecções micóticas muitas vezes não apresentam alterações clínicas e lesões macroscópicas características que permitam um diagnóstico presuntivo (Kaufman 1992, Jensen et al 1996, o qual possa ser confirmado pela cultura micológica, que é a técnica padrão-ouro (Lima et al 1977). Consequentemente, o agente causal pode ser encontrado ou suspeitado somente durante o exame histopatológico dos tecidos afetados (Jensen et al 1996).…”

Section: Introductionunclassified

“…Consequentemente, o agente causal pode ser encontrado ou suspeitado somente durante o exame histopatológico dos tecidos afetados (Jensen et al 1996). Nesses casos, o reconhecimento detalhado das características histomorfológicas e histoquímicas desses fungos constitui-se na principal ferramenta de diagnóstico etiológico.…”

Section: Introductionunclassified

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Características histomorfológicas e histoquímicas determinantes no diagnóstico da criptococose em animais de companhia

et al. 2014

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Pesq. Vet. Bras. 34(3):261-269, março 2014 261 RESUMO.-Sete casos de criptococose (seis gatos e um cão) foram estudados para estabelecer as características histomorfológicas e histoquímicas determinantes no diagnóstico histopatológico dessa condição. Os dados complementares relacionados à epidemiologia, aos aspectos clínicos, à localização das lesões e às alterações macroscópicas foram obtidos dos protocolos de necropsias e biópsias. Na histologia, as leveduras foram observadas no interior de macrófagos ou livres no parênquima, associadas à reação inflamatória linfo-histioplasmocítica que variou de escassa a acentuada. Pela técnica de hematoxilina-eosina (HE) as leveduras eram arredondadas, com célula central contendo um núcleo, circundada por um halo claro (cápsula geralmente não corada). As técnicas histoquímicas do ácido periódico de Schiff (PAS), Grocott e Fontana-Masson (FM) foram utilizadas e evidenciaram a parede das células das leveduras. Pelo FM observou-se a melanina presente nessas células. As téc- Seven cases of cryptococosis (six cats and one dog) were studied to establish the determining histomorphological and histochemical characteristics in the histopathological diagnosis of this condition. Additional data related to the epidemiology, clinical aspects, sites of the lesions, and gross findings were obtained from the necropsy and biopsy protocols. Histologically, yeasts were observed inside macrophages or free in the parenchyma, associated with scarse to severe lymphohistioplasmacytic inflammatory reaction. In the hematoxylin-eosin (HE) sections, the yeasts were round, with a central cell containing a nucleus, surrounded by a clear halo (usually non-stained capsule). The techniques of periodic Schiff acid (PAS), Groccot (GMS), and Fontana-Masson (FM) were utilized and demonstrated the wall of the yeast cells. The FM stain showed the melanin present in these cells. The Alcian blue and Mayer's mucicarmin stains showed mainly the yeast polysaccharide capsule. The diameter of the cells ranged from 1.67 to 10.00µm and the full diameter of the encapsulated yeasts varied between 4.17 e 34.16µm. Yeast buddings were better observed through the PAS stain and were narrow based, simple or multiple, mainly in the opposite poles of the cells or forming chains. The definitive diagnosis of cryptococosis was established through the histopathological exam, based on the specific morphology of the agent (encapsulated yeast) and on histochemical proprieties, mostly in the cases without fungal culture.

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Section: Discussionunclassified

Section: Introductionunclassified

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Características histomorfológicas e histoquímicas determinantes no diagnóstico da criptococose em animais de companhia

et al. 2014

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“…Unfortunately, many of the filamentous fungi cannot be reliably differentiated from one another with any of the routine fungal stains (Phillips and Weiner, 1987;Buckley et al, 1992;Reed et al, 1993;Jensen et al, 1993;Jensen et al, 1996a;Fenelon et al, 1999), because the appearance of fungi in sections is affected by steric orientation, age of the fungi, type of infected tissue, the number of fungal elements present, and the host response Jensen, 1994). It has also been reported that sometimes, and most often in chronic lesions containing non-viable hyphae, staining may only be weak, or in overstained sections fungal fragments may be masked.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, some inflammatory reactions may suggest the possibility of a mycotic infection, even though no fungus is identified in tissue sections (Jensen, 1994;Jensen et al, 1996a). For these reasons, immunohistochemical techniques are increasingly used for obtaining specific diagnosis in cases of mycosis in birds (Carrasco et al, 1993;Jensen et al, 1997) as well as in mammals.…”

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confidence: 99%

Immunohistochemical detection of fungal elements in the tissues of goslings with pulmonary and systemic aspergillosis

Beytut

Özcan

Erginsoy

2004

Acta Veterinaria Hungarica

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Nineteen goslings with pulmonary and systemic aspergillosis were the subject of the study. The lungs and air sacs were the main sites affected by the disease, and were generally characterised by diffuse yellowish-white granulomas. In 7 cases with pulmonary and air-sac involvement the granulomas were scattered to the serosal linings of the gastrointestinal and upper respiratory tracts, to the liver, spleen and kidneys, and in two cases also to the bursa of Fabricius, musculus (m.) longus colli and adventitia of aorta. The granulomas were often characterised by a necrotic centre surrounded by heterophils, macrophages, lymphocyte and plasma cells, and in late granulomas by multinucleated foreign-body giant cells, and again by an outer thin fibrous capsule. Numerous fungal hyphae were found within the necrotic debris of the granulomas by Gridley and PAS staining techniques. Immunohistochemistry reliably confirmed aspergillosis in all of the cases. Fungal elements in the lungs of goslings severely affected by the disease stained heavily within the centre of the granulomas, whereas few antigens reacted in the chronic cases. Fungal fragments, which were not discernible using routine fungal stains, reacted clearly in the cytoplasm of macrophages and giant cells. Thus, although fungal elements within the granulomas were histologically indicative of aspergillosis, immunohistochemistry also had to be applied to obtain a definitive diagnosis of the disease and to differentiate it from many of the filamentous fungi.

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The Use of Immunohistochemistry to Improve Sensitivity and Specificity in the Diagnosis of Systemic Mycoses in Patients With Haematological Malignancies

1997

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The original histomorphological diagnoses in a series of 34 mycotic lesions from 23 patients with haematological malignancies were re‐evaluated by immunohistochemistry. A panel of antibodies was used to identify the agents of aspergillosis, candidosis, fusariosis, scedosporiosis (pseudallescheriosis), and zygomycosis. Apart from improving the diagnosis of aspergillosis, candidosis, and zygomycosis, the application of immunohistochemistry also disclosed three lesions of aspergillosis which had been overlooked during the original screening. It is concluded that the use of immunohistochemistry for the diagnosis of common opportunistic mycoses will not only increase diagnostic specificity, but will also reveal more tissue infections than the conventional histomorphological examination of traditionally stained sections. © 1997 by John Wiley & Sons, Ltd.

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Diagnosis of systemic mycoses by specific immunohistochemical tests

Cited by 73 publications

References 71 publications

Características histomorfológicas e histoquímicas determinantes no diagnóstico da criptococose em animais de companhia

Características histomorfológicas e histoquímicas determinantes no diagnóstico da criptococose em animais de companhia

Immunohistochemical detection of fungal elements in the tissues of goslings with pulmonary and systemic aspergillosis

The Use of Immunohistochemistry to Improve Sensitivity and Specificity in the Diagnosis of Systemic Mycoses in Patients With Haematological Malignancies

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