Diagnosis of tuberculous lymphadenitis by FNAC, microbiological methods and PCR: a comparative study

Aljafari, Alfatih Saifudinn; Khalil, E. A. G.; Hag, I. A. El; Mutlu, İbrahim; Elsafi, Mohamed Elhassan; Hussein, Abdinafic Mohamud; Elkhidir, Isam M; Sulaiman, G. S.; El-Hassan, A. M.

doi:10.1111/j.1365-2303.2003.00119.x

Cited by 80 publications

(64 citation statements)

References 16 publications

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“…Comparable observations have been reported in other studies (5,7,23). These findings are analogous to those reported for the use of FNA for the diagnosis of tuberculous lymphadenitis (2,8,15,27). Also, a recent study by Phillips et al (24) reports PCR as the most sensitive technique for confirming the presence of M. ulcerans in FNA of nonulcerative BU lesions.…”

Section: Discussionsupporting

confidence: 77%

Fine-Needle Aspiration, an Efficient Sampling Technique for Bacteriological Diagnosis of Nonulcerative Buruli Ulcer

et al. 2009

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Invasive punch or incisional skin biopsy specimens are currently employed for the bacteriological confirmation of the clinical diagnosis of Buruli ulcer (BU), a cutaneous infectious disease caused by Mycobacterium ulcerans. The efficacy of fine-needle aspirates (FNA) using fine-gauge needles (23G by 25 mm) for the laboratory confirmation of BU was compared with that of skin tissue fragments obtained in parallel by excision or punch biopsy. In three BU treatment centers in Benin, both types of diagnostic material were obtained from 33 clinically suspected cases of BU and subjected to the same laboratory analyses: i.e., direct smear examination, IS2404 PCR, and in vitro culture. Twenty-three patients, demonstrating 17 ulcerative and 6 nonulcerative lesions, were positive by at least two tests and were therefore confirmed to have active BU. A total of 68 aspirates and 68 parallel tissue specimens were available from these confirmed patients. When comparing the sensitivities of the three confirmation tests between FNA and tissue specimens, the latter yielded more positive results, but only for PCR was this significant. When only nonulcerative BU lesions were considered, however, the sensitivities of the confirmation tests using FNA and tissue specimens were not significantly different. Our results show that the minimally invasive FNA technique offers enough sensitivity to be used for the diagnosis of BU in nonulcerative lesions.

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Section: Discussionsupporting

confidence: 77%

Fine-Needle Aspiration, an Efficient Sampling Technique for Bacteriological Diagnosis of Nonulcerative Buruli Ulcer

et al. 2009

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“…13,18 This study had a lower false negative rate in TB diagnosis when compared to a study by Aljafari et al in Sudan where he found a false negative rate of 38% using a bigger gauge needle. 19 The difference, probably can be attributed to the differences in sizes of needles used, since the bigger the needle the higher the chances of haemorrhage distorting the cytological picture. There was an excellent kappa agreement of 0.85 between FNAC and histology results in the diagnosis of tuberculosis.…”

Section: Diagnostic Accuracymentioning

confidence: 99%

Diagnostic accuracy of fine needle aspiration cytology in providing a diagnosis of cervical lymphadenopathy among HIV-infected patients

Muyanja¹,

Kalyesubula²,

Namukwaya³

et al. 2015

Afr H. Sci.

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Background: Opportunistic infections and malignancies cause lymphadenopathy in HIV-infected patients. The use and accuracy of fine needle aspiration cytology in diagnosing of cervical lymphadenopathy among HIV-infected patients is not well studied in Uganda. Objective: The aim of this study was to determine the diagnostic accuracy of fine needle aspiration cytology in providing a diagnosis of cervical lymphadenopathy among HIV-infected patients in Uganda. Methods: We consecutively recruited adult HIV-infected patients with cervical lymphadenopathy admitted to Mulago Hospital medical wards. Clinical examination, fine needle aspiration and lymph node biopsy were performed. We estimated the sensitivity, specificity; negative and positive predictive values using histology as the gold standard. Results: We enrolled 108 patients with a mean age of 33 years (range, 18-60), 59% were men and mean CD4 was 83(range, 22-375) cells/mm

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“…FNAC of the lymph node was done under clinical guidance with a 23-gauge needle under aseptic conditions [6,7] . The aspirate was divided into 5 parts.…”

Section: Fine-needle Aspiration Cytologymentioning

confidence: 99%

Molecular Diagnosis of Tubercular Lymphadenopathy from Fine-Needle Aspirates in Pediatric Patients

Gupta

Bhake²

2017

Acta Cytologica

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Objectives: The diagnosis of peripheral tubercular lymphadenopathy (TBLN) in pediatric patients is often a challenge because features evident on fine-needle aspiration cytology (FNAC) or tissue biopsy can be deceptive for the reason that they result from an immunological response. This study aimed to evaluate polymerase chain reaction (PCR) for Mycobacterium tuberculosis complex (MTBC) in pediatric patients under clinical suspicion for TBLN and to assess its role in the evaluation of cases cytodiagnosed as reactive lymphoid hyperplasia. Methods: This was a cross-sectional study conducted on 45 pediatric patients clinically suspected and unsuspected for TBLN. FNAC, culture on Löwenstein-Jensen medium, and real-time PCR were performed. Comparative values with reference to the culture were calculated. Results: Cytology had a sensitivity and specificity of 38.5 and 87.5%, respectively. Real-time PCR had a sensitivity and specificity of 84.6 and 81.3%, respectively. Of the 32 cases with a cytodiagnosis of reactive lymphoid hyperplasia, 53% were positive both on PCR and culture for M. tuberculosis; the φ value of 0.93 demonstrated a strong association between these 2 methods. Conclusion: Real-time PCR is useful in detecting MTBC in pediatric patients, and it also helps in the diagnosis of cases missed on FNAC.

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Diagnosis of tuberculous lymphadenitis by FNAC, microbiological methods and PCR: a comparative study

Cited by 80 publications

References 16 publications

Fine-Needle Aspiration, an Efficient Sampling Technique for Bacteriological Diagnosis of Nonulcerative Buruli Ulcer

Fine-Needle Aspiration, an Efficient Sampling Technique for Bacteriological Diagnosis of Nonulcerative Buruli Ulcer

Diagnostic accuracy of fine needle aspiration cytology in providing a diagnosis of cervical lymphadenopathy among HIV-infected patients

Molecular Diagnosis of Tubercular Lymphadenopathy from Fine-Needle Aspirates in Pediatric Patients

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