Diagnosis of vibriosis in the era of genomics: lessons from invertebrates

Roux, Frédérique Le

doi:10.20506/rst.35.1.2431

Cited by 5 publications

(3 citation statements)

References 49 publications

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“…The genitors that had experienced low-level natural selection were wild oysters recruited outside farming areas (Supplementary Table 1 ). The juvenile offspring of the 15 biparental families produced were subjected to an ‘natural’ experimental infection mimicking disease transmission in the wild 12 , 13 , 29 (Supplementary Fig. 1 ).…”

Section: Resultsmentioning

confidence: 99%

Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters

et al. 2018

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Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly influenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial and necessary step of infection of oyster haemocytes by the Ostreid herpesvirus OsHV-1 µVar. Viral replication leads to the host entering an immune-compromised state, evolving towards subsequent bacteraemia by opportunistic bacteria. We propose the application of our integrative approach to decipher other multifactorial diseases that affect non-model species worldwide.

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Section: Resultsmentioning

confidence: 99%

Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters

et al. 2018

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“…The bacterial genera colonizing oysters during POMS are conserved across environments suggesting functional complementarity within the pathobiota [15]. Members of the Vibrionaceae family are the best characterized bacteria in the POMS pathobiota [16][17][18][19][20]. Several Vibrio species have been shown to have virulence functions in this disease [19,21].…”

Section: Fatal Polymicrobial Synergy Has Been Reported Between Viruse...mentioning

confidence: 99%

Cooperation and cheating orchestrateVibrioassemblages and polymicrobial synergy in oysters infected with OsHV-1 virus

Oyanedel

Lagorce

Bruto

et al. 2023

Preprint

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Polymicrobial diseases significantly impact the health of humans and animals but remain understudied in natural systems. We recently described the Pacific Oyster Mortality Syndrome (POMS), a polymicrobial disease that impacts oyster production and is prevalent worldwide. Analysis of POMS-infected oysters on the French North Atlantic coast revealed that the disease involves co-infection with the endemic ostreid herpesvirus 1 (OsHV-1) and virulent bacterial species such as Vibrio crassostreae. However, it is unknown whether consistent Vibrio populations are associated with POMS in different regions, how Vibrio contribute to POMS, and how they interact with the OsHV-1 virus during pathogenesis. We resolved the Vibrio population structure in oysters from a Mediterranean ecosystem and investigated their functions in POMS development. We find that Vibrio harveyi and Vibrio rotiferianus are the predominant species found in OsHV-1-diseased oysters and show that OsHV-1 is necessary to reproduce the partition of the Vibrio community observed in the field. By characterizing the interspecific interactions between OsHV-1, V. harveyi and V. rotiferianus, we find that only V. harveyi synergizes with OsHV-1. When co-infected, OsHV-1 and V. harveyi behave cooperatively by promoting mutual growth and accelerating oyster death. V. harveyi showed high virulence potential in oysters and dampened host cellular defenses, making oysters a more favorable niche for microbe colonization. We next investigated the interactions underlying the co-occurrence of diverse Vibrio species in diseased oysters. We found that V. harveyi harbors genes responsible for the biosynthesis and uptake of a key siderophore called vibrioferrin. This important resource promotes the growth of V. rotiferianus, a cheater that efficiently colonizes oysters during POMS without costly investment in host manipulation nor metabolite sharing. By connecting field-based approaches, laboratory infection assays and functional genomics, we have uncovered a web of interdependencies that shape the structure and function of the POMS pathobiota. We showed that cooperative behaviors contribute to synergy between bacterial and viral co-infecting partners. Additional cheating behaviors further shape the polymicrobial consortium. Controlling such behaviors or countering their effects opens new avenues for mitigating polymicrobial diseases.

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“…The bacterial genera colonizing oysters during POMS are conserved across environments suggesting functional complementarity within the pathobiota ( 15 ). Members of the Vibrionaceae family are the best characterized bacteria in the POMS pathobiota ( 16 – 20 ). Several Vibrio species have been shown to have virulence functions in this disease ( 19 , 21 ).…”

mentioning

confidence: 99%

Cooperation and cheating orchestrate Vibrio assemblages and polymicrobial synergy in oysters infected with OsHV-1 virus

Oyanedel,

Lagorce,

Bruto

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Polymicrobial infections threaten the health of humans and animals but remain understudied in natural systems. We recently described the Pacific Oyster Mortality Syndrome (POMS), a polymicrobial disease affecting oyster production worldwide. In the French Atlantic coast, the disease involves coinfection with ostreid herpesvirus 1 (OsHV-1) and virulent Vibrio . However, it is unknown whether consistent Vibrio populations are associated with POMS in different regions, how Vibrio contribute to POMS, and how they interact with OsHV-1 during pathogenesis. By connecting field-based approaches in a Mediterranean ecosystem, laboratory infection assays and functional genomics, we uncovered a web of interdependencies that shape the structure and function of the POMS pathobiota. We show that Vibrio harveyi and Vibrio rotiferianus are predominant in OsHV-1-diseased oysters and that OsHV-1 drives the partition of the Vibrio community observed in the field. However only V. harveyi synergizes with OsHV-1 by promoting mutual growth and accelerating oyster death. V. harveyi shows high-virulence potential and dampens oyster cellular defenses through a type 3 secretion system, making oysters a more favorable niche for microbe colonization. In addition, V. harveyi produces a key siderophore called vibrioferrin. This important resource promotes the growth of V. rotiferianus , which cooccurs with V. harveyi in diseased oysters, and behaves as a cheater by benefiting from V. harveyi metabolite sharing. Our data show that cooperative behaviors contribute to synergy between bacterial and viral coinfecting partners. Additional cheating behaviors further shape the polymicrobial consortium. Controlling cooperative behaviors or countering their effects opens avenues for mitigating polymicrobial diseases.

show abstract

Diagnosis of vibriosis in the era of genomics: lessons from invertebrates

Cited by 5 publications

References 49 publications

Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters

Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters

Cooperation and cheating orchestrateVibrioassemblages and polymicrobial synergy in oysters infected with OsHV-1 virus

Cooperation and cheating orchestrate Vibrio assemblages and polymicrobial synergy in oysters infected with OsHV-1 virus

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