Diagnosis of visceral leishmaniasis

Srivastava, Pankaj; Dayama, Anand; Mehrotra, Sanjana; Sundar, Shyam

doi:10.1016/j.trstmh.2010.09.006

Cited by 236 publications

(205 citation statements)

References 58 publications

Supporting

Mentioning

181

Contrasting

Unclassified

Order By: Relevance

“…The sensitivity and specificity of the strip test were 82.4% and 100%, this finding higher than our study [14].…”

Section: Discussioncontrasting

confidence: 82%

Incidence of Leishmania Donovani Infection among Agropastoralists Tribes in Um-alkhare and Bazura Villages, Gedarif State, Eastern Sudan

Elamin¹,

M²,

Ad³

et al. 2017

J Trop Dis

View full text Add to dashboard Cite

Visceral leishmaniasis (VL) is a life-threatening parasitic disease, transmitted by a sand fly. A survey aimed to estimate the VL incidence in Um-alkhare and Bazura villages located in endemic southern part of Gedaref State, Eastern Sudan, in the period among (March 2014-February 2015. This study conducted among Agro-pastoralists tribes in the two villages comparing the epidemiological and clinical spectrum of VL infection. One hundred seventy five (109 male and 66 females) clinical suspected for VL were examined in the two rural hospitals, their age range between (3-48 years old). Bone marrow (BM) and lymph node (LN) smears were aspirated for parasitological examination and the serum was tested by using rk39 for Leishmania donovani antibodies. Socio-demographic and other determinants governing VL were also studied. The incidence rate of visceral leishmaniasis from was 42.8/1,000 person per year,and 57.1% for the prevalence rate. Microscopic examination for VL was confirmed in 64 patients (49 males, 15 females) from BM and LN aspirate smears. rK39 test gave prevalence rate of 36.6% and 42.3% VL antibodies for BM and LN specimen respectively. Sensitivity and specificity for rK39 was determined to be 79% and 70%, respectively. Patient's positive for microscopic tests showed clinical signs and symptoms such as, severe irregular fever, splenomegaly and LN enlargement. Hausa tribe has a highest VL infection (26.7%), followed by Masaleet (18.7%). Infection rate was reported to occur during October coinciding with high transmission season. It was also observed that prevailing Balantis trees, cracking soil and Leishmania reservoir have direct correlation with VL infection. Further genetic studies based on wide community survey are highly recommended to verify susceptibility of different agro-pastoralists tribes to VL infection.

show abstract

“…The sensitivity and specificity of the strip test were 82.4% and 100%, this finding higher than our study [14].…”

Section: Discussioncontrasting

confidence: 82%

Incidence of Leishmania Donovani Infection among Agropastoralists Tribes in Um-alkhare and Bazura Villages, Gedarif State, Eastern Sudan

Elamin¹,

M²,

Ad³

et al. 2017

J Trop Dis

View full text Add to dashboard Cite

show abstract

“…A similar PCR-based technique was applied in the diagnosis of VL in HIV patients, with good results [28].…”

Section: Molecular Methods: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

“…The sensitivity of the bone marrow stained with Giemsa is about 60% to 85%. In splenic aspirates, the sensitivity is higher (93%) [28], but sampling is associated with a risk of fatal hemorrhage in inexperienced hands. To increase the sensitivity, fluorescent dye-conjugated antibodies can be used [29].…”

Section: Culture and Microscopic Observationmentioning

confidence: 99%

“…In this respect, this method has reported 100% sensitivity and 96% specificity in immunocompromised patients [39]. In another study, 87% specificity and 85% sensitivity were obtained for primary VL in HIV-coinfected patients, and the method had predictive capabilities in the follow-up of treatment and detection of subclinical infection in Leishmania/HIV co-infected [28].…”

Section: Antigen Detection In Urinementioning

confidence: 99%

See 1 more Smart Citation

Diagnosis of leishmaniasis

Elmahallawy

Martínez

Rodríguez‐Grangér

et al. 2014

J Infect Dev Ctries

154

119

View full text Add to dashboard Cite

Leishmaniasis is a clinically heterogeneous syndrome caused by intracellular protozoan parasites of the genus Leishmania. The clinical spectrum of leishmaniasis encompasses subclinical ( not apparent), localized (skin lesion), and disseminated (cutaneous, mucocutaneous, and visceral) infection. This spectrum of manifestations depends on the immune status of the host, on the parasite, and on immunoinflammatory responses. Visceral leishmaniasis causes high morbidity and mortality in the developing world. Reliable laboratory methods become mandatory for accurate diagnosis, especially in immunocompromised patients such as those infected with HIV. In this article, we review the current state of the diagnostic tools for leishmaniasis, especially the serological test.

show abstract

“…104,105 Serological tests have a high diagnostic value for VL diagnosis in immunocompetent patients, [106][107][108] but their value is limited in HIV-infected patients. [105][106][107][108][109] According to data obtained from a study evaluating the accuracy of invasive and noninvasive tests for diagnosis of VL in a large series of HIV-infected patients at a reference center in Brazil, serological tests, such as indirect fluorescent antibody test and ELISA, showed lower sensitivity (,60%) when compared to the direct agglutination test (DAT), the sensitivity of which was 85%. 105 DAT is considered a highly sensitive and easy-to-use test and may be a good alternative for screening VL in HIV-infected patients.…”

Section: Leishmaniasismentioning

confidence: 99%