Diagnostic Accuracy of Coronary Calcium Score Less than 100 in Excluding Coronary Artery Disease

Hanifehpour, Reza; Motevalli, Marzieh; Shahriari, Mona; Ghasabeh, Mounes Aliyari

doi:10.5812/iranjradiol.16705

Cited by 15 publications

(12 citation statements)

References 19 publications

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“…Currently, CAD incidence is still a main cause of mortality in the world [22]. Endothelial cells are the essential components of various inflammatory diseases, such as psoriasis, diabetes, cancer and rheumatoid arthritis, and chronic inflammation is related to increased atherosclerosis and accelerated cardiovascular mortality and morbidity [23].…”

Section: Discussionmentioning

confidence: 99%

Effects of MicroRNA-499 On the Inflammatory Damage of Endothelial Cells During Coronary Artery Disease Via the Targeting of PDCD4 Through the NF-Κβ/TNF-α Signaling Pathway

Zhang¹,

He²,

Shi³

2017

Cell Physiol Biochem

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Background/Aims: This study aimed to analyze the impact of microRNA-499 (miR-499) on the inflammatory damage of endothelial cells during coronary artery disease (CAD) via the targeting of PDCD4 through the NF-kB/ TNF-α signaling pathway. Methods: A total of 216 CAD patients (CAD group) and 90 healthy people (normal group) were enrolled in our study. Endothelial cells were collected and assigned into normal, OX-LDL, negative control (NC), miR-499 inhibitor, miR-499 mimic, PDCD4 siRNA, and miR-499 inhibitor + PDCD4 siRNA groups. The qRT-PCR and western blotting were performed to detect the mRNA and protein expression levels of PDCD4 and miR-499. The MTT assay was performed to determine cell viability, ELISA was performed to determine the expression levels of inflammatory factors, and flow cytometry assay to evaluate cell apoptosis. Results: Increased miR-499 expression and decreased PDCD4 expression in the plasma were observed in the CAD group compared with the normal group, demonstrating a negative correlation between miR-499 and PDCD4. Compared to the normal and miR-499 inhibitor groups, the survival rate of cells and PDCD4 expression were decreased; and the expressions of miR-499, IL-6, IL-8, IL-1β, TNF-α, NF-kB, VCAM-1, ICAM-1 and MCP-1 and the apoptosis rate were all elevated in the OX-LDL, NC, miR-499 mimic, PDCD4 siRNA and miR-499 inhibitor + PDCD4 siRNA groups. Compared to the OX-LDL, NC and miR-499 inhibitor + PDCD4 siRNA groups, PDCD4 expression and the survival rate of cells were increased; and the IL-6, IL-8, IL-1β, TNF-α, NF-κB, VCAM-1, ICAM-1 and MCP-1 expression levels and the apoptosis rate were all reduced in the miR-499 inhibitor group. In the PDCD4 siRNA group, PDCD4 expression and the survival rate of cells were lower, and the expression levels of IL-6, IL-8, IL-1β, TNF-α, NF-κB, VCAM-1, ICAM-1 and MCP-1 and the apoptosis rate were all higher compared with the miR-499 mimic group. In the miR-499 inhibitor + PDCD4 siRNA group, PDCD4 expression and the survival rate of cells were higher, and the expression levels of IL-6, IL-8, IL-1β, TNF-α, NF-κB, VCAM-1, ICAM-1, and MCP-1 and the apoptosis rate were all lower than those in the PDCD4 siRNA group. Conclusion: Down-regulated miR-499 expression increased PDCD4 expression and protected endothelial cells from inflammatory damage during CAD by inhibiting the NF-κB/TNF-α signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Effects of MicroRNA-499 On the Inflammatory Damage of Endothelial Cells During Coronary Artery Disease Via the Targeting of PDCD4 Through the NF-Κβ/TNF-α Signaling Pathway

Zhang¹,

He²,

Shi³

2017

Cell Physiol Biochem

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“…cHd is a major cause of mortality in the world (34). Endothelial cells are involved in a number of inflammatory diseases, including psoriasis, diabetes, cancer and rheumatoid arthritis (35).…”

Section: Discussionmentioning

confidence: 99%

Protective effect of microRNA‑381 against inflammatory damage of endothelial cells during coronary heart disease by targeting CXCR4

Huang

Hong

et al. 2020

Mol Med Report

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coronary heart disease (cHd) is the leading cause of human morbidity and mortality worldwide. Microrna (mirna) profiling is an innovative method of identifying biomarkers for many diseases and may be a powerful tool in the diagnosis and treatment of cHd. The present study aimed to analyze the effects of miRNA (miR)-381 on the inflammatory damage of endothelial cells during cHd. a total of 21 patients with cHd and 21 healthy control patients were enrolled in this study. reverse transcription-quantitative PCR, western blotting and immunofluorescence assays were conducted to examine the expression levels of mir-381, c-X-c chemokine receptor type 4 (cXcr4), Bcl-2, Bax, cleaved-caspases-3 and-9, p38, erK1/2 and JnK. cell Counting Kit-8, EdU and flow cytometry experiments were performed to evaluate cell proliferation and apoptosis. an ELISA was adopted to determine the expressions of inflammatory factors (interleukins-8,-6 and-1β, and tumor necrosis factor-α). in addition, a dual-luciferase reporter assay was used to determine the relationship between mir-381 and cXcr4. decreased mir-381 expression and increased cXcr4 expression in the plasma were observed in the cHd group compared with the normal group, which indicated a negative relationship between mir-381 and cXcr4. overexpression of mir-381 significantly promoted the proliferation and inhibited the apoptosis of oxidized low-density lipoprotein (oX-ldl)-induced human umbilical vein endothelial cells (HuVecs) through mitogen-activated protein kinase pathway by targeting and inhibiting cXcr4. Furthermore, overexpression of miR-381 reduced the release of inflammatory factors in oX-ldl-induced HuVecs. By contrast, reduced expression of mir-381 exerted the opposite effects, which were subsequently reversed by silencing cXcr4 expression. results from the present study indicated that mir-381 was a cHd-related factor that may serve as a potential molecular target for cHd treatment.

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“…Although we have cited some previous studies about the impact of IR algorithms on the Agatston score-based risk category, we were not sure whether this effect extended to evaluation efficiency of the risk category for coronary artery disease. A few studies with a combination of Agatston score and CCTA [ 33 , 34 ] have shown that coronary calcium scoring provided useful information in the diagnosis of coronary artery stenosis. It has been reported that the prevalence of significant coronary artery stenoses increased with increasing calcium amounts and extensive calcium may be associated with a high prevalence of significant coronary artery stenoses [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Sinogram-Affirmed Iterative Reconstruction Negatively Impacts the Risk Category Based on Agatston Score: A Study Combining Coronary Calcium Score Measurement and Coronary CT Angiography

Wang

Zhao

et al. 2020

BioMed Research International

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Purpose. To assess the impact of sinogram-affirmed iterative reconstruction (SAFIRE) on risk category for coronary artery disease by combining coronary calcium score measurement and coronary CT angiography (CCTA). Materials and Methods. Eighty-nine patients (64.0% male) older than 18 years (64.4±10.3 years) underwent coronary artery calcium scanning and prospectively ECG-triggered sequential CCTA examination. All raw data acquired in coronary artery calcium scanning were reconstructed by both filtered back projection (FBP) and SAFIRE algorithms with 5 different levels. Objective image quality and calcium quantification were evaluated and compared between FBP and all SAFIRE levels by the Sphericity Assumed test or Greenhouse-Geisser ε correction coefficient. Coronary artery stenosis was assessed in CCTA. Risk categories of all patients and of the patients with coronary artery stenosis in CCTA were compared between FBP and all SAFIRE levels by the Friedman test. Results. The reconstruction protocol from traditional FBP to SAFIRE 5 was associated with a gradual reduction in CT value and image noise (P<0.001) but associated with a gradual improvement in the signal-to-noise ratio (P<0.001). There was a gradual reduction in coronary calcification quantification (Agatston score: from 73.5 in FBP to 38.1 in SAFIRE 5, P<0.001) from traditional FBP to SAFIRE 5. There was a significant difference for the risk category between FBP and all levels of SAFIRE in all patients (from 3.5 in FBP to 3.2 in SAFIRE 5, P<0.001) and in the patients with coronary artery stenosis in CCTA (from 4.0 in FBP to 3.6 in SAFIRE 5, P<0.001). Conclusions. SAFIRE significantly reduces coronary calcification quantification compared to FBP, resulting in the reduction of risk categories based on the Agatston score. The risk categories of the patients with coronary artery stenosis in CCTA may also decline. Thus, SAFIRE may lead risk categories to underestimate the existence of significant coronary artery stenosis.

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Diagnostic Accuracy of Coronary Calcium Score Less than 100 in Excluding Coronary Artery Disease

Cited by 15 publications

References 19 publications

Effects of MicroRNA-499 On the Inflammatory Damage of Endothelial Cells During Coronary Artery Disease Via the Targeting of PDCD4 Through the NF-Κβ/TNF-α Signaling Pathway

Effects of MicroRNA-499 On the Inflammatory Damage of Endothelial Cells During Coronary Artery Disease Via the Targeting of PDCD4 Through the NF-Κβ/TNF-α Signaling Pathway

Protective effect of microRNA‑381 against inflammatory damage of endothelial cells during coronary heart disease by targeting CXCR4

Sinogram-Affirmed Iterative Reconstruction Negatively Impacts the Risk Category Based on Agatston Score: A Study Combining Coronary Calcium Score Measurement and Coronary CT Angiography

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