Diagnostic Accuracy of Group A Streptococcus Rapid Antigen Detection Test on Middle Ear Fluid in Children With Acute Otitis Media With Spontaneous Perforation: A Prospective Multicenter Evaluation

Cohen, Robert; Varon, Emmanuelle; Bidet, Philippe; Cohen, Jérémie F.; Béchet, Stéphane; Couloigner, V.; Michot, A.-S.; Guiheneuf, C.; Bonacorsi, Stéphane; Lévy, Corinne

doi:10.1097/inf.0000000000004009

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…Although the design of the present study is not the same, rapid antigen tests that are developed with immunochromatographic methods for the rapid diagnosis of S. pyogenes are widely used for diagnostic purposes [ 36 , 37 , 38 ]. In their study, Reijtman et al attempted to develop an immunochromatographic method to detect S. pyogenes antigen directly from clinical samples and positive blood culture bottles.…”

Section: Discussionmentioning

confidence: 99%

“…GAS is known to be among the most common bacterial pathogens, especially in children who are older than 3 years, and the incidence of GAS infections has increased in invasive and non-invasive forms in recent years. GAS also frequently causes middle ear infections in children between the ages of 3 months and 15 years [ 37 ]. Cohen et al conducted a multicenter immunochromatographic test trial in children who had middle ear infections and found that the performance of this test had a very high sensitivity [97.3%] and specificity [100%] when compared to the culture method.…”

Section: Discussionmentioning

confidence: 99%

“…Cohen et al conducted a multicenter immunochromatographic test trial in children who had middle ear infections and found that the performance of this test had a very high sensitivity [97.3%] and specificity [100%] when compared to the culture method. They also reported that diagnosis, especially made with rapid antigen tests, contributed to the prescription of narrow-spectrum antibiotics, which may reduce the development of antibiotic resistance [ 37 ]. Solvik et al aimed to evaluate the diagnostic performance of two rapid antigen tests, the QuickVue Dipstick Strep A test and the DIAQUICK Strep A blue dipstick under real-life conditions.…”

Section: Discussionmentioning

confidence: 99%

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Fabrication of a Microfluidic Test Device with a 3D Printer and Its Combination with the Loop Mediated Isothermal Amplification Method to Detect Streptococcus pyogenes

Kirkoyun Uysal,

Eryildiz,

Demirci

2024

Micromachines

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New rapid, reliable, and cost-effective alternative systems are needed for the rapid diagnosis of Streptococcus pyogenes. The aim of this study was to fabricate a microfluidic test device to detect Streptococcus pyogenes by combining the Loop-mediated isothermal amplification method via a 3D printer. Microfluidic test devices were designed in CATIA V5 Release 16 software, and data were directly transferred to a 3D printer and produced using the FDM method with biocompatible PLA filament. The S. pyogenes ATCC 19615 and different ATCC strains was used. Following identification by classical culture methods, a 0.5 McFarland suspension was prepared from the colonies, and DNA isolation was performed from this liquid by a boiling method. S. pyogenes specific speB gene was used to desing LAMP primer sets in PrimerExplorer V5 software and tested on a microfluidic device. LAMP reactions were performed on microfluidic device and on a microcentrifuge tube separately. Both results were analyzed using the culture method as the standard method to diagnostic values. Melting curve analysis of the amplicons of the LAMP reactions performed on a LightCycler 480 system to detect amplification. Among the 50 positive and 100 negative samples, only four samples were found to be false negative by LAMP reaction in a microcentrifuge tube, while eight samples were found to be false negative by LAMP reaction on a microfluidic device. Six samples were found to be false positive by the LAMP reaction in the microcentrifuge tube, while ten samples were found to be false positive by the LAMP reaction on a microfluidic chip. The sensitivity, specificity, positive predictive value, and negative predictive value of the LAMP reactions performed in the microcentrifuge tube and on the microfluidic device were 92–84%, 94–90%, 88.46–80.77%, and 95.92–91.84%, respectively. The limit of detection (LOD) was found to be the same as 1.5 × 102 CFU/mL and the limit of quantification (LOQ) values of the LAMP reactions were performed on the microcentrifuge tube and on the microfluidic device were 2.46 × 102–7.4 × 102 CFU/mL, respectively. Cohen’s kappa (κ) values of the LAMP reactions were performed on the microcentrifuge tube and on the microfluidic device were 0.620–0.705, respectively. In conclusion, our data showed that the LAMP method can be combined with microfluidic test device to detect S. pyogenes, this microfluidic device can be manufactured using 3D printers and results are close to gold standard methods. These devices can be combined with LAMP reactions to detect different pathogens where resources are limited and rapid results are required.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Fabrication of a Microfluidic Test Device with a 3D Printer and Its Combination with the Loop Mediated Isothermal Amplification Method to Detect Streptococcus pyogenes

Kirkoyun Uysal,

Eryildiz,

Demirci

2024

Micromachines

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Group A Streptococcal meningitis in children: a short case series and systematic review

Dou,

Li,

et al. 2024

Eur J Clin Microbiol Infect Dis

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Background Group A streptococcal(GAS) meningitis is a severe disease with a high case fatality rate. In the era of increasing GAS meningitis, our understanding about this disease is limited. Purpose To gain a better understanding about GAS meningitis. Methods Five new cases with GAS meningitis were reported. GAS meningitis related literatures were searched for systematic review in PUBMED and EMBASE. Case reports and case series on paediatric cases were included. Information on demographics, risk factors, symptoms, treatments, outcomes, and emm types of GAS was summarized. Results Totally 263 cases were included. Among 100 individuals, 9.9% (8/81) had prior varicella, 11.1% (9/81) had anatomical factors, and 53.2% (42/79) had extracranial infections. Soft tissue infections were common among infants (10/29, 34.5%), while ear/sinus infections were more prevalent in children ≥ 3 years (21/42, 50.0%). The overall case fatality rate (CFR) was 16.2% (12/74). High risk of death was found in patients with shock or systemic complications, young children(< 3 years) and cases related to hematogenic spread. The predominate cause of death was shock(6/8). Among the 163 patients included in case series studies, ear/sinus infections ranged from 21.4 to 62.5%, while STSS/shock ranged from 12.5 to 35.7%, and the CFR ranged from 5.9 to 42.9%. Conclusions A history of varicella, soft tissue infections, parameningeal infections and CSF leaks are important clinical clues to GAS in children with meningitis. Young children and hematogenic spread related cases need to be closely monitored for shock due to the high risk of death.

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Antimicrobial treatment of ENT infections

Cohen,

Madhi,

Thollot

et al. 2023

Infectious Diseases Now

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Diagnostic Accuracy of Group A Streptococcus Rapid Antigen Detection Test on Middle Ear Fluid in Children With Acute Otitis Media With Spontaneous Perforation: A Prospective Multicenter Evaluation

Cited by 4 publications

References 18 publications

Fabrication of a Microfluidic Test Device with a 3D Printer and Its Combination with the Loop Mediated Isothermal Amplification Method to Detect Streptococcus pyogenes

Fabrication of a Microfluidic Test Device with a 3D Printer and Its Combination with the Loop Mediated Isothermal Amplification Method to Detect Streptococcus pyogenes

Group A Streptococcal meningitis in children: a short case series and systematic review

Antimicrobial treatment of ENT infections

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