Diagnostic accuracy of hematoxylin-eosin staining in comparison to calretinin and S100 for the assessment of ganglion cells in rectal biopsy

Aziz, Zainab Waleed; Hajjar, Bassam Khalid Al; Hajjar, Ziyad Ahmed Abed Al; Almola, Ehsan Mahmood Abdullah; Jawher, Nazar Mohammed Taher; Muhammad, Radhwan Khudhur Muhammad

doi:10.47391/jpma.3368

Cited by 1 publication

(1 citation statement)

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“…Although it has been shown that the addition of IHC to conventional HE may improve diagnostic sensitivity and specificity [15], this has, to the best of our knowledge, never been proven in randomized studies. In a study by Gonzalo et al, a retrospective analysis was conducted of 17 rectal suction biopsies that were deemed inconclusive at HE staining.…”

Section: Discussionmentioning

confidence: 94%

Full-Thickness Rectal Biopsy in Children Suspected of Having Hirschsprung’s Disease: The Inconclusive Biopsy

Korsager,

Bjørn,

Ellebæk

et al. 2023

Children

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The diagnosis of Hirschsprung’s disease relies on histologically proven aganglionosis and nerve trunk hypertrophy in rectal biopsies. Although the frequency of inconclusive biopsies is relatively low, it is a relevant clinical problem. The aim of the present study was to investigate whether a re-evaluation of archived full-thickness biopsies (FTBs) stained with hematoxylin and eosin (HE), together with immune histochemical (IHC) staining, would be diagnostic in biopsies otherwise deemed inconclusive at initial examination with HE only. A total of 34 inconclusive biopsies in 31 patients were identified. From each tissue block, three slices were cut and stained with HE, S100 and calretinin. A blinded pathologist examined the tissue samples. At re-evaluation, one patient was found positive for HD and 11 negative for HD with both HE and IHC staining, respectively. In all 12 cases, the result was confirmed by the final diagnosis at a 5-year follow-up. The rest of the cases were deemed inconclusive. A re-evaluation of the remaining tissue from the biobank might have saved one third of the children from a re-biopsy. The value of adding IHC to conventional HE staining is dubious.

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Section: Discussionmentioning

confidence: 94%