Diagnostic Accuracy of Novel and Traditional Rapid Tests for Influenza Infection Compared With Reverse Transcriptase Polymerase Chain Reaction

Merckx, Joanna; Wali, Rehab; Schiller, Ian; Caya, Chelsea; Gore, Geneviève; Chartrand, Caroline; Dendukuri, Nandini; Papenburg, Jesse

doi:10.7326/m17-0848

Cited by 246 publications

(233 citation statements)

References 141 publications

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“…The majority of clinical diagnostic labs have shifted to using rapid molecular tests, which may be performed as a laboratory-developed realtime reverse transcription PCR (rRT-PCR) panel or cartridge-based assay. 64,65 These methods demonstrate excellent analytic parameters and have a turn-around time on the order of minutes to hours. However, the shortcomings of these rapid molecular tests were made evident during the 2009 pandemic.…”

Section: Clinical Laboratory Diagnosticsmentioning

confidence: 99%

Emerging respiratory infections: The infectious disease pathology of SARS, MERS, pandemic influenza, and Legionella

Bradley

Bryan

2019

Seminars in Diagnostic Pathology

112

126

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A B S T R A C TLower respiratory infections remain one of the top global causes of death and the emergence of new diseases continues to be a concern. In the first two decades of the 21st century, we have born witness to the emergence of newly recognized coronaviruses that have rapidly spread around the globe, including severe acute respiratory syndrome virus (SARS) and Middle Eastern respiratory syndrome virus (MERS). We have also experienced the emergence of a novel H1N1 pandemic influenza strain in 2009 that caused substantial morbidity and mortality around the world and has transitioned into a seasonal strain. Although we perhaps most frequently think of viruses when discussing emerging respiratory infections, bacteria have not been left out of the mix, as we have witnessed an increase in the number of infections from Legionella spp. since the organisms' initial discovery in 1976. Here, we explore the basic epidemiology, clinical presentation, histopathology, and clinical laboratory diagnosis of these four pathogens and emphasize themes in humans' evolving relationship with our natural environment that have contributed to the infectious burden. Histology alone is rarely diagnostic for these infections, but has been crucial to bettering our understanding of these diseases. Together, we rely on the diagnostic acumen of pathologists to identify the clinicopathologic features that raise the suspicion of these diseases and lead to the early control of the spread in our populations. (A. Bryan). Seminars in DiagnosticPathology 36 (2019) 152-159 0740-2570/

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Section: Clinical Laboratory Diagnosticsmentioning

confidence: 99%

Emerging respiratory infections: The infectious disease pathology of SARS, MERS, pandemic influenza, and Legionella

Bradley

Bryan

2019

Seminars in Diagnostic Pathology

112

126

View full text Add to dashboard Cite

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“…Rapid antigen tests are available for influenza A and B and respiratory syncytial virus (RSV), but these tests have low sensitivity and specificity. 1,2 The authors declare no conflicts of interest.…”

Section: Introductionmentioning

confidence: 99%

Combining Procalcitonin and Rapid Multiplex Respiratory Virus Testing for Antibiotic Stewardship in Older Adult Patients With Severe Acute Respiratory Infection

Lee

Chang

Mao

et al. 2020

Journal of the American Medical Directors Association

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“…Traditional rapid diagnostics, such as immunoassays, produce quick results and are simple to perform but have sub-optimal sensitivity (Reviewed in [1]). NAATs, which are rapid and have enhanced sensitivity, are considered the method of choice by many and are recommended by IDSA Guidelines [2][3][4]. However, performance differences have been observed among commercial NAATs, particularly after 2014 when sequence divergence in the matrix gene of A(H3N2) viruses emerged [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Both FluB and RSV now target the matrix gene and the AdV hexon gene target is designed to detect all AdV genotypes. The gene targets for hMPV and PIV 4 are the nucleocapsid genes, while those for PIV [1][2][3] and RV are the hemagglutinin-neuraminidase region and the 5′ UTR, respectively. This test system also expands the menu of virus detected with the inclusion of a RV and PIV4.…”

Section: Introductionmentioning

confidence: 99%

Panther Fusion® Respiratory Virus Assays for the detection of influenza and other respiratory viruses

Stellrecht

Cimino

Wilson

et al. 2019

Journal of Clinical Virology

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Background: Nucleic acid amplification tests (NAATs), such as PCR, are preferred for respiratory virus testing, due to superior diagnostic accuracy and faster turnaround time. Panther Fusion® Respiratory Assays (Fusion), which includes FluA/B/RSV (FFABR), Paraflu and AdV/hMPV/RV, offers a modular approach to syndromic testing on a fully automated platform while improving gene targets and expanding the test menu. Objectives and study design: We evaluated Fusion using 275 consecutive nasopharyngeal specimens previously used in an analysis of five PCRs, as well as 225 archived specimens. Results: Of the combined 500 specimens, 134 were positive for influenza A (FluA), 54 for FluB, 65 for RSV, 64 for parainfluenza (PIV), 24 for adenovirus (AdV), 21 for humanmetapneumovirus (hMPV), and 40 for rhinovirus (RV) with Fusion. Of the positive samples Fusion correlated with historical results for all but one, despite multiple freeze-thaws cycles of this collection. Fusion was positive for an additional 33 samples, including 11 FluAs, 7 RSVs, 3 PIV3s, 3 AdV, 6 hMPV and 3 RVs. These samples were retested with corresponding Prodesse (Pro) assays using quadruple sample volume. This resolver test confirmed Fusion results for an additional 4 FluAs, 4 RSVs, 1 PIV3 and 3 AdVs. The sensitivity and specificity ranges of Fusion were 99-100% and 98-100%. Limit of detection (LOD) analyses were performed on a variety of Flu isolates. The LODs ranged from 2.69 to 2.99 log copies/ml and demonstrated superior LOD as compared to previously published data for some assays or to concurrent analyses with two new commercial tests.

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Diagnostic Accuracy of Novel and Traditional Rapid Tests for Influenza Infection Compared With Reverse Transcriptase Polymerase Chain Reaction

Abstract: Québec Health Research Fund and BD Diagnostic Systems.

Cited by 246 publications

References 141 publications

Emerging respiratory infections: The infectious disease pathology of SARS, MERS, pandemic influenza, and Legionella

Emerging respiratory infections: The infectious disease pathology of SARS, MERS, pandemic influenza, and Legionella

Combining Procalcitonin and Rapid Multiplex Respiratory Virus Testing for Antibiotic Stewardship in Older Adult Patients With Severe Acute Respiratory Infection

Panther Fusion® Respiratory Virus Assays for the detection of influenza and other respiratory viruses

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