Diagnostic accuracy of sFlt1/PlGF ratio as a marker for preeclampsia

Nikuei, Pooneh; Rajaei, Minoo; Roozbeh, Nasibeh; Mohseni, Fatemeh; Poordarvishi, Fatemeh; Azad, Mohsen; Haidari, Solmaz

doi:10.1186/s12884-020-2744-2

Cited by 50 publications

(32 citation statements)

References 25 publications

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“…The sFlt-1/PIGF ratio was not significantly increased in severe PE than mild PE (p = 0.0795) as documented in other studies. Our finding was however, similar to that documented by Nikuei et al, who observed that although the ratio was higher in severe PE than mild PE, the difference was not statistically significant, p = 0.389 [20]. Chaiworapongsa and co-workers [14] documented that increasing sFlt-1 correlates with disease severity, this was not observed in this study.…”

Section: Discussionsupporting

confidence: 88%

“…One of such markers is the alterations in maternal serum concentrations of anti-angiogenic (sFlt-1) and pro-angiogenic factors (VEGF and PlGF) [11]. Interestingly, the sFlt-1/PlGF ratio has been demonstrated to show better performance than single markers in predicting the risk of PE [19] [20]. The alteration of the balance between concentration of pro-angiogenic factors, VEGF and PIGF, and anti-angiogenic factors like sFlt-1 and Endoglin has been documented in the pathophysiology of preeclampsia [21].…”

Section: Introductionmentioning

confidence: 99%

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Placenta Growth Factor and Soluble Fms-Like Tyrosine Kinase 1 in Preeclampsia and Normotensive Pregnant Nigerian Women

Gbadegesin¹,

Agbara²,

Rabiu³

et al. 2021

OJOG

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Background: Preeclampsia (PE) is still one of the leading causes of maternal/perinatal morbidity/mortality in Nigeria. Imbalance between placenta growth factor (PLGF) and soluble fms-like tyrosine kinase 1 (sFlt1) has been reportedly present both before and after the manifestation of PE; however, Nigerian data regarding these angiogenesis-related substances are lacking. We here attempted to determine the maternal serum level of PLGF and sFlt1 and sFlt1/PLGF ratio in PE vs. non-PE women in Lagos State University Teaching Hospital, Nigeria. Methods: An observational cross-sectional study was made on 75 women with PE and 75 age-gestational-age matched women without PE, as case and control, respectively. Levels of sFlt-1, PIGF and the sFlt-1: PIGF ratio was compared between the two. Results: Serum levels of Flt-1 and sFlt1/PIGF ratio were significantly higher in PE patients (6581.86 ± 865.75, and 146.42 ± 92.43) than in the normotensive control (4584.52 ± 1479.6 and 11.60 ± 6.42). PIGF was significantly lower in PE patients (70.14 ± 51.03) than the normotensives (494.06 ± 475.8). There were positive and negative correlations between the sFlt-1 and PLGF respectively and mean arterial blood pressure. Conclusion: Serum sFlt-1, sFlt1/PIGF ratio was significantly higher and PIGF levels were significantly lower in PE than normotensive control in Nigerian population.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Placenta Growth Factor and Soluble Fms-Like Tyrosine Kinase 1 in Preeclampsia and Normotensive Pregnant Nigerian Women

Gbadegesin¹,

Agbara²,

Rabiu³

et al. 2021

OJOG

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“…PE progresses rapidly. There are currently no specific laboratory indicators to diagnose and grade the severity of PE patients, which may have an impact on the subsequent treatment of patients (8,9). The anti-angiogenic factor sFlt-1 is secreted by the placenta and is involved in vascular regulation.…”

Section: Discussionmentioning

confidence: 99%

“…The ROC curve results in this study show that sFlt-1, CXCL16, and LCN-2 all have good sensitivity for the diagnosis of PE, among which LCN-2 is the best for the diagnosis of PE, as it reached a diagnostic sensitivity of 93.33% and an AUC of 0.831. Insufficient blood supply to the placenta leads to the increase in sFlt-1 content, and the increase in sFlt-1 leads to angiogenesis dysfunction, damaged endothelial cells, and subsequent inflammation and PE (9). Inflammation and endothelial damage can induce high expression of LCN-2, which can possibly cause vascular sclerosis to aggravate PE (14).…”

Section: Discussionmentioning

confidence: 99%

Expression and significance of serum soluble fms-like tyrosine kinase 1 (sFlt-1), CXC chemokine ligand 16 (CXCL16), and lipocalin 2 (LCN-2) in pregnant women with preeclampsia

Li¹,

Ling²,

Mei³

et al. 2021

Ann Palliat Med

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Background: To explore the value of serum soluble fms-like tyrosine kinase 1 (sFlt-1), CXC chemokine ligand 16 (CXCL16), and lipocalin 2 (LCN-2) in the diagnosis and grading of preeclampsia (PE).Methods: A total of 186 patients with PE diagnosed and treated in our hospital were included. According to the disease severity, the patients were divided into the mild PE group (99 cases) and the severe PE group (87 cases). A total of 72 healthy pregnant women who underwent antenatal care were selected as the healthy control group. The levels of serum sFlt-1, CXCL16, and LCN-2 before medication were compared among the patients, and the diagnosis and grading value of the above 3 indicators were analyzed. Results: For PE patients vs. healthy controls, the levels of sFlt-1 (132.71±14.49 vs. 68.43±9.28 μg/L), CXCL16 (2.15±0.35 vs. 0.61±0.12 μg/L), and LCN-2 (70.81±8.25 vs. 19.22±3.14 μg/L) were all significantly higher in PE patients than in the healthy controls (P<0.05). For severe PE vs. mild PE, the levels of sFlt-1 (142.16±20.23 vs. 124.41±10.36 μg/L), CXCL16 (2.87±0.59 vs. 1.51±0.28 μg/L), and LCN-2 (90.76±10.16vs. 53.27±6.19 μg/L) in the severe PE group were higher than those in the mild PE group (P<0.05). Receiver operating characteristic curve (ROC) analysis showed that when the cut-off values of sFlt-1, CXCL16, and LCN-2 were 99.65, 1.36, and 0.84 μg/L, respectively, the diagnostic efficacy of PE was the highest. With these cut-off values, the diagnostic sensitivities of sFlt-1, CXCL16, and LCN-2 were 86.67%, 73.33%, and 93.33%, respectively. The specificities of sFlt-1, CXCL16, and LCN-2 were 80.00%, 86.67%, and 60.00%, respectively. The areas under the curves (AUC) of sFlt-1, CXCL16, and LCN-2 were 0.764, 0.769, and 0.831, respectively. When the cut-off values for sFlt-1, CXCL16, and LCN-2 were 135.16, 2.24, and 70.38 μg/L, respectively, the efficacy was the highest in distinguishing mild and severe PE. With these cut-off values, the AUC values of sFlt-1, CXCL16, and LCN-2 were 0.837, 0.808, and 0.869, respectively.Conclusions: sFlt-1, CXCL16, and LCN-2 have certain significance in the diagnosis and grading of PE.Among them, LCN-2 has the highest correlation with the diagnosis and grading of PE.

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“…As a member of the VEGF family, PLGF is considered as a biomarker in PE and its development. 19,49,50 Uteroplacental ischemia in PE can significantly reduce the PLGF expression in nonhuman primate, and PLGF treatment can reduce blood pressure and proteinuria. 51,52 However, a report demonstrated that PLGF is not statistically significant compared with the control group at the mRNA level.…”

Section: Discussionmentioning

confidence: 99%

High glucose condition inhibits trophoblast proliferation, migration and invasion by downregulating placental growth factor expression

Tao

Xia

Chen

et al. 2020

J of Obstet and Gynaecol

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Aim This study aimed to investigate the effect of high glucose (HG) level on the proliferation, migration and invasion of trophoblasts and determine the role of placental growth factor (PLGF) in the process. Methods HTR8‐S/Vneo was treated with different concentrations of d‐glucose (0, 10, 15, 20, 25 and 30 μM) at different times (0, 6, 12 and 24 h). qRT‐PCR and Western blot analyses were used to measure PLGF expression. The protein level of PLGF was measured by immunofluorescence. Cell proliferation was assessed with CCK‐8 analysis. Wound healing and transwell assays were used to evaluate cell migration and invasion. Intercellular ROS was detected with DCFH‐DA. Results After d‐glucose treatment, the viability decreased in 25 and 30 μM groups. The HG group (25 μM) showed inhibited cell migration and invasion ability. The mRNA and protein levels of PLGF decreased under HG condition. Elevated ROS production was also detected in the HG group. Knocked‐down PLGF expression enhanced increased ROS production and decreased cell migration and invasion, which reverted to the original levels after PLGF was overexpressed. Conclusion High glucose treatment inhibited HTR8‐S/Vneo viability, migration and invasion by downregulating PLGF expression.

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Diagnostic accuracy of sFlt1/PlGF ratio as a marker for preeclampsia

Cited by 50 publications

References 25 publications

Placenta Growth Factor and Soluble Fms-Like Tyrosine Kinase 1 in Preeclampsia and Normotensive Pregnant Nigerian Women

Placenta Growth Factor and Soluble Fms-Like Tyrosine Kinase 1 in Preeclampsia and Normotensive Pregnant Nigerian Women

Expression and significance of serum soluble fms-like tyrosine kinase 1 (sFlt-1), CXC chemokine ligand 16 (CXCL16), and lipocalin 2 (LCN-2) in pregnant women with preeclampsia

High glucose condition inhibits trophoblast proliferation, migration and invasion by downregulating placental growth factor expression

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