Diagnostic and prognostic relevance of microparticles in peripheral and uterine blood of patients with endometrial cancer

Abstract

Objectives: Exosomes-microvesicles which are secreted by living cells-can be produced from different cell types and detected in various body fluids. They are the carriers of intercellular information which regulate tumor microenvironment and are considered to be involved in tumor progression and metastasis. Cancer cells can secrete more exosomes than healthy cells, and are expected to be potential tools for tumor diagnosis and treatment. Material and methods: In this report, we present the results of microparticle analysis in peripheral and uterine blood of patients with endometrial cancer. To the best of our knowledge, this study has been the first to report microvesicle status in peripheral and uterine blood samples. The aim of the study was to determine the amount of total (TF+), endothelial (CD144+) and monocytic (CD14+) microparticles. The counting of the selected microparticles in citrate plasma was performed using flow cytometry on the BD Canto II cytometer. Results: We found that the total amount of microparticles in cancer patients was much higher than in healthy controls. Moreover, microparticle count in uterine blood was higher than in peripheral blood of patients with endometrial cancer. We also demonstrated that the amount of microparticles correlates with the histologic grade and clinical stage of the tumor. Conclusions: The most interesting finding in this work was the high level of TF, CD144 and CD14 MPs in uterine blood samples. Thus we can consider the monocyte-macrophage-derived MPs as a candidate marker of endometrial cancer and maybe very critical part of the endometrial carcinogenesis.

Extracellular Vesicles from Hyperammonemic Rats Induce Neuroinflammation and Motor Incoordination in Control Rats

“…Similar increases have been reported in other pathological situations, mostly associated to inflammation. The amount of EVs is increased in serum of patients with alcoholic hepatitis [38], β-thalassemia [39], endometrial [40] or lung cancer, correlating with tumor stage [41], obesity and diabetes mellitus [42], and chronic obstructive pulmonary disease, associated with systemic inflammation [43] and in serum of children with autism spectrum disorder [26]. Inflammation seems to trigger the increased amount of EVs in most of these situations.…”

Liquid biopsy in endometrial cancer

“…Pełnią ważną funkcję w komunikacji międzykomórkowej, wpływając na procesy zarówno fizjologiczne, jak i patologiczne. Są uwalniane przez wszystkie typy komórek, w warunkach prawidłowych, jak również patologicznych, jednak w przypadku komórek nowotworowych proces ten zachodzi w większym stopniu w porównaniu z komórkami prawidłowymi (2,19,31,32) . Krążące egzosomy po raz pierwszy zostały opisane w 1981 roku jako pozakomórkowe pęcherzyki uwalniane przez komórki nowotworowe.…”

“…Exosomes are involved in cell-to-cell signaling, and play a role in both physiological and pathological processes. Exosomes are released by all cell types, both under normal and pathological conditions, but in cancer cells the process is more pronounced compared to normal cells (2,19,31,32) . Circulating exosomes were first described in 1981 as extracellular vesicles released by cancer cells.…”

“…The authors concluded that MPs were potentially a candidate marker of endometrial cancer and perhaps a critical element of endometrial carcinogenesis. However, they also noted that further studies were needed to verify their clinical utility, and suggested they should be focused on the uterine cavity, where the proliferation of endometrial cancer begins (32) .…”

“…To date, this new research method has been used in a range of tumors including non-small cell lung cancer, colorectal cancer, breast, liver and bile duct cancer (29,36) . użyteczności klinicznej; analizy te powinny być skoncentrowane na jamie macicy, gdzie rozpoczyna się proliferacja raka endometrium (32) .…”