Diagnostic and Prognostic Value of Serum Lactate Dehydrogenase (LDH) and LDH Isoenzymes in Canine Lymphoma

Clinical relevance of serial determinations of lactate dehydrogenase activity used to predict recurrence in dogs with lymphoma

“…22 It has become clear that use of only anatomic staging provides inadequate prognostic information, thereby making it difficult for clinical investigators to identify useful factors associated with the disease-free interval and overall survival. 12,13 In the study reported here, serum LDH activity at the time of initial lymphoma diagnosis was not associated with disease-free interval or overall survival interval, which is in contrast with another study 16 in dogs in which the LDH activity at diagnosis was associated with a shorter survival interval. 22,[25][26][27][28][29][30][31] In humans with non-Hodgkin lymphoma, the correlation between LDH activity at time of diagnosis and disease aggressiveness has been clearly established; increased serum LDH activities are closely associated with marked disease activity and diminished response to treatment.…”

“…14 Consequently, evaluation of LDH activity is considered important in the diagnosis, staging, and management for human patients with non-Hodgkin lymphoma. 16,18,19 In 1 study, 16 high LDH activities at the time of lymphoma diagnosis were associated with a shorter duration of survival, regardless of clinical stage or grade of the malignancy. 16,18,19 In 1 study, 16 high LDH activities at the time of lymphoma diagnosis were associated with a shorter duration of survival, regardless of clinical stage or grade of the malignancy.…”

“…13 Additionally, elevated serum LDH activities in patients without clinical signs may precede the onset of the clinical stage for occult lymphoma. [13][14][15] Measurement of LDH activity in small animals with lymphoma has gained attention [16][17][18][19] ; however, little information is available on the clinical importance of LDH activity in the canine species regarding prognosis or follow-up monitoring. [13][14][15] Measurement of LDH activity in small animals with lymphoma has gained attention [16][17][18][19] ; however, little information is available on the clinical importance of LDH activity in the canine species regarding prognosis or follow-up monitoring.…”

Longitudinal study of the effects of chronic hypothyroidism on skeletal muscle in dogs

“…Isoenzymatic fractions of LDH were determined via agarose gel electrophoresis at 100 V for 20 minutes as described, 17 with individual fractions expressed as percentages of the total LDH activities. Venous blood samples were immediately centrifuged for 15 minutes at 1,500 X g at 4 o C. Plasma samples were then frozen at -80 o C until analyzed for determination of activities of CK, AST, ALT, lactate, and total LDH and total, free, and esterified carnitine concentrations.…”

Reference intervals for the activity of lactate dehydrogenase and its isoenzymes in the serum and cerebrospinal fluid of healthy canines

“…Serum LD activities have been used as diagnostic and prognostic indicators for both systemic and localized diseases in human medicine, including malignancies such as lymphoma and leukemia . As in people, canine serum LD concentrations may be used as a prognostic tool in lymphoma cases . In addition, serum LD levels have been demonstrated to be significantly elevated in dogs with metastatic mammary tumors .…”