Diagnostic and Prognostic Values of MANF Expression in Hepatocellular Carcinoma

Abstract

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors, and its prognosis is still poor. Mesencephalic astrocyte-derived neurotrophic factor (MANF) plays a key role in endoplasmic reticulum stress. ER stress plays a key role in HCC carcinogenesis. To confirm the clinical and prognostic value of MANF in HCC, we investigated the expression level of MANF in HCC as recorded in databases, and the results were verified by experiment. Survival analysis was probed by the Kaplan–Meier method. Cox regression models were used to ascertain the prognostic value of MANF in HCC tissue microarray. The diagnostic value of MANF in HCC was evaluated by receiver operating characteristic curve analysis. Potential correlation between MANF and selected genes was also analyzed. Results showed that MANF was overexpressed in HCC. Patients with high MANF expression levels had a worse prognosis and higher risk of tumor recurrence. Furthermore, the expression level of MANF had good diagnostic power. Correlation analysis revealed potential regulatory networks of MANF in HCC, laying a foundation for further study of the role of MANF in tumorigenesis. In conclusion, MANF was overexpressed in HCC and related to the occurrence and development of HCC. It is a potential diagnostic and prognostic indicator of HCC.

Identification and Validation of Novel Biomarkers for Hepatocellular Carcinoma, Liver Fibrosis/Cirrhosis and Chronic Hepatitis B via Transcriptome Sequencing Technology

“…However, it remains unknown whether the expression of ITGB2 is elevated in hepatocellular carcinoma. He et al 39 showed that MANF was overexpressed in HCC, but Liu et al 40 found that the mRNA and protein levels of MANF were lower in HCC tissues than in adjacent non-cancer tissues. Thus, we hypothesize that the differential expression of MANF is associated with liver cancer.…”

Identification of potential plasma markers for hepatitis B virus‐related chronic hepatitis and liver fibrosis/cirrhosis

“…21 At present, various candidate biomarkers or discriminative models had been proposed, and most of them were concentrated at end-stage liver diseases, especially on HCC. 22,23 controls. Finally, our findings were verified through an independent validation cohort of 230 participants.…”

Expression of cyclin-dependent kinases and their clinical significance with immune infiltrates could predict prognosis in colorectal cancer

“…Gene expression prognostic signature for high-grade serous ovarian cancer was investigated by resampling/cross-validation method with Cox regression analysis followed by Kaplan–Meier analysis and log-rank testing [ 62 ]. Similarly, He et al [ 63 ] probed the survival analysis of the expression level of MANF in HCC using the Kaplan–Meier method. In addition, Cox regression models were used to ascertain the prognostic value.…”