Diagnostic and therapeutic options in recurrent implantation failure

Moustafa, Sarah; Young, Steven L.

doi:10.12688/f1000research.22403.1

Cited by 52 publications

(32 citation statements)

References 91 publications

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“…However, despite the increasing trend of the procedure, it has still been facing challenges in terms of a low success rate, which only 10-40% globally [1] and 20-50% locally in Indonesia [2]. Apart from maternal factors, another reason for this sub-optimal outcome is the high number of embryo transfer failures [3], [4], [5]. Failure transfer is related to the insufficient embryo quality to develop reaches pregnancy [6].…”

Section: Introductionmentioning

confidence: 99%

Carrier RNA (cRNA) Enhances dsDNA Recovery Extracted from Small Volume Spent Embryo Culture Medium

Sundari¹,

Iffanolida²,

Mutia³

et al. 2021

Int. J. Adv. Sci. Eng. Inf. Technol.

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Embryo spent culture medium has been intensively investigated, considering its promising feature for non-invasive bioanalytical techniques in in-vitro fertilization (IVF). Despite, isolating DNA from such samples is quite challenging due to its small volume. Carrier RNA is reported to exhibit DNA retrieval effects and commonly employed in various limited biological samples, but there are no reports regarding its benefit on embryo media. Therefore, we aim to evaluate the competence of cRNA on isolated DNA from embryo medium and analyzed its optimal volume as there are also no records respecting its ideal volume to obtain decent outcomes. Results showed that cRNA significantly increases DNA amounts in the cRNA treated group (p<0.001), but the D-4/D-5 medium yielded similar (p=0.684). Pearson test demonstrated no correlation between cRNA volume vs. total retrieved DNA (r=0.760, p=0.80), and Whole Genome Amplification (WGA) was shown to increase DNA in the treated group (p=0.022), but not in the untreated group (p=0.128). Additionally, electrophoresis successfully resulting in a thick and thin band of TH01 locus signifies the cRNA competence. In conclusion, our study suggests that cRNA addition is essential in embryo medium extraction as it increases initial DNA that crucial for downstream application. However, the optimal volume could not be determined in the current study since the initial amount of DNA in the medium is unknown. Obtained findings are expected to be a new input for subsequent research on DNA extraction.

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Section: Introductionmentioning

confidence: 99%

Carrier RNA (cRNA) Enhances dsDNA Recovery Extracted from Small Volume Spent Embryo Culture Medium

Sundari¹,

Iffanolida²,

Mutia³

et al. 2021

Int. J. Adv. Sci. Eng. Inf. Technol.

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“…After a review of the titles and abstracts, 154 studies were identified as potentially eligible for inclusion. After a full review, we excluded 19 systematic reviews or meta-analysis 2,5,22,23,37,[46][47][48][49][50][51][52][53][54][55][56][57][58][59] , 8 case reports [60][61][62][63][64][65][66][67] , 4 letters to the editor [68][69][70][71] and 81 original studies [references and reasons for exclusion are reported in Table 1]. Data on the efficacy of therapies and interventions for RIF were extracted from the remaining 42 articles 8,12,13,18,20,21,24,27,28,31,75, .…”

Section: Resultsmentioning

confidence: 99%

Efficacy of therapies and interventions for repeated embryo implantation failure: a systematic review and meta-analysis

Busnelli

Somigliana

Cirillo

et al. 2021

Sci Rep

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The aim of the present systematic review and meta-analysis was to assess the effect of the different therapeutic options for repeated embryo implantation failure (RIF) on a subsequent IVF cycle outcome. Twenty-two RCTs and nineteen observational studies were included. Pooling of results showed a beneficial effect of intrauterine PBMC infusion on both CPR (RR 2.18; 95% CI 1.58–3.00; p < 0.00001; OR 2.03; 95% CI 1.22–3.36; p = 0.006) and LBR (RR 2.41; 95% CI 1.40–4.16; p = 0.002; OR 3.73; 95% CI 1.13–12.29; p = 0.03), of subcutaneous G-CSF administration on CPR (RR 2.29; 95% CI 1.58–3.31; p < 0.0001) and of intrauterine PRP infusion on CPR (RR 2.45; 95% CI 1.55–3.86; p = 0.0001). Observational studies also demonstrated a positive effect of IVIG and intrauterine hCG infusion on both CPR and LBR and of atosiban on CPR. Studies investigating intrauterine G-CSF infusion, LMWH, intravenous intralipid, hysteroscopy, blastocyst-stage ET, ZIFT, PGT-A and AH failed to observe an impact on IVF outcome. The quality of the evidence that emerged from RCTs focused on intrauterine PBMC infusion and subcutaneous G-CSF administration was moderate. For all other therapies/interventions it varied from low to very low. In conclusion, intrauterine PBMC infusion and subcutaneous G-CSF administration are the most promising therapeutic options for RIF. However, further well conducted RCTs are necessary before their introduction into clinical practice.

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“…Thus, endometriosis was not considered to be a cause of infertility in some of the patients with early-stage endometriosis. In addition, a large number of patients with implantation failure were potentially included in the patients treated with IVF [ 43 , 44 ]. Since it has been reported that CE is related to implantation failure [ 45 , 46 ], and because we hypothesized that the occurrence of EMT was related to implantation failure, IVF patients were targeted in the present study.…”

Section: Discussionmentioning

confidence: 99%

Induction of the epithelial-mesenchymal transition in the endometrium by chronic endometritis in infertile patients

et al. 2021

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Background The purpose of the present study was to evaluate the relationship between chronic endometritis and the epithelial-mesenchymal transition in the endometrium of infertile patients in the implantation phase. Methods Endometrial biopsy specimens from 66 infertility patients were analyzed. The presence of chronic endometritis was investigated by immunostaining for CD138. Immunohistochemical staining for E-cadherin, N-cadherin, Slug, and Snail was performed, and the expression profiles were statistically analyzed according to the presence of chronic endometritis. When the loss of E-cadherin expression and/or the positive expression of N-cadherin was detected, the specimen was considered epithelial-mesenchymal transition-positive. Epithelial-mesenchymal transition-positive cases were also statistically analyzed according to the presence of chronic endometritis. The characteristics of the patients in the epithelial-mesenchymal transition-positive and epithelial-mesenchymal transition-negative groups were compared. The association between variables, including age, body mass index, gravidity, parity, and each causative factor of infertility and epithelial-mesenchymal transition positivity was analyzed. Results The rates of the loss of E-cadherin expression, the gain of N-cadherin and epithelial-mesenchymal transition positivity were significantly higher in chronic endometritis patients. The expression of Slug, cytoplasmic Snail, and nuclear Snail was also detected at significantly higher rates in chronic endometritis patients. Chronic endometritis were related to the epithelial-mesenchymal transition. Conclusion The epithelial-mesenchymal transition was frequently detected in the endometrium in infertile patients with chronic endometritis. Since the epithelial-mesenchymal transition is associated with chronic endometritis, the epithelial-mesenchymal transition appears to be involved in the alteration of mechanisms of implantation.

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Diagnostic and therapeutic options in recurrent implantation failure

Cited by 52 publications

References 91 publications

Carrier RNA (cRNA) Enhances dsDNA Recovery Extracted from Small Volume Spent Embryo Culture Medium

Carrier RNA (cRNA) Enhances dsDNA Recovery Extracted from Small Volume Spent Embryo Culture Medium

Efficacy of therapies and interventions for repeated embryo implantation failure: a systematic review and meta-analysis

Induction of the epithelial-mesenchymal transition in the endometrium by chronic endometritis in infertile patients

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