Diagnostic approaches for oculoglandular tularemia: advantages of PCR

Kantardjiev, Todor; Padeshki, Plamen; Ivanov, Ivan N.

doi:10.1136/bjo.2007.117523

Cited by 35 publications

(27 citation statements)

References 10 publications

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“…1B). Despite the high sensitivity of the assay (10), no RD1 amplicons were obtained from any of the FLE-positive samples, suggesting that they lack this region or at least have a significantly different RD1 sequence. Our finding suggests that F. tularensis can be readily distinguished from the three FLEs described in this study by RD1.…”

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confidence: 97%

Detection of New Francisella-Like Tick Endosymbionts in Hyalomma spp. and Rhipicephalus spp. (Acari: Ixodidae) from Bulgaria

Ivanov

Mitkova

Reye

et al. 2011

Appl Environ Microbiol

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mentioning

confidence: 97%

Detection of New Francisella-Like Tick Endosymbionts in Hyalomma spp. and Rhipicephalus spp. (Acari: Ixodidae) from Bulgaria

Ivanov

Mitkova

Reye

et al. 2011

Appl Environ Microbiol

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“…In this study F. tularensis was detected by PCR in the majority of lymph node aspirates and even in surgically extracted conjunctival granuloma [26].…”

Section: Discussionmentioning

confidence: 97%

Tularemia in Bulgaria 2003-2004

Komitova

Nenova

Padeshki

et al. 2010

J Infect Dev Ctries

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Introduction: Tularemia is an uncommon but potentially fatal zoonosis. А second outbreak of tularemia in Bulgaria, about 40 years after the first, occurred in 1997 in two western regions, near the Serbian border. In 2003 tularemia reemerged in the same foci. This retrospective study aimed to evaluate the clinical characteristics and the efficacy of antibiotic therapy in a tularemia resurgence in the Slivnitza region in [2003][2004]. Methodology: A total of 26 cases were evaluated. Using medical records, the following data were collected for all patients: symptoms, physical signs, and microbiology results of agglutination tests, cultures and PCR assays. Results: Twenty-four of 26 suspected tularemia patients were laboratory confirmed by agglutination test and/or culture. Fifteen (57.7%) patients had clinical presentation compatible with oropharyngeal, 8 (30.8%) with glandular, and 3 (11.5%) with oculoglandular tularemia. The most frequent symptoms were swollen neck (84.6%) and sore throat (76.9%). Lymphadenopathy (100%) was the most common finding. Francisella tularensis (F. tularensis) was detected by PCR, providing a definitive diagnosis in 82.3% of the cases. All the patients were treated with antibiotics considered effective against F. tularensis; however, therapeutic failure was observed in 23.1% of the cases, which was related to a delay in the initiation of antibiotics. Conclusion: The tularemia outbreak in west Bulgaria near the Serbian border was probably food-borne, associated with a surge in the rodent population. The oropharyngeal form was the most common. Although the disease runs a benign course, late initiation of antimicrobial therapy might delay complete recovery.

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“…A description of the use of PCR for the diagnosis of oculoglandular tularemia was published recently, noting positive results from conjunctival swab in one patient and a positive PCR result from a conjunctival nodule in another [50]. In these two patients, PCR testing of the blood was also positive.…”

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confidence: 97%

Tularemia: current diagnosis and treatment options

Hepburn

Simpson

2008

Expert Review of Anti-infective Therapy

117

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Tularemia is an infection caused by Francisella tularensis with a worldwide distribution and diverse clinical manifestations. Limitations in both culture and serologic testing have led to substantial research into new diagnostic techniques and their clinical application, with PCR testing as the best example. This review focuses on the utility of culture, PCR and serologic testing for tularemia. In addition, we also review the evidence to support different therapeutic options for tularemia, highlighting both the most effective supporting evidence for therapeutic recommendations as well as gaps in current knowledge. We conclude the article with suggestions regarding potential areas for future research.

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Diagnostic approaches for oculoglandular tularemia: advantages of PCR

Abstract: Some of the proposed laboratory diagnostic strategies (swab PCR) are not invasive and could represent a new approach for resolving rare and hard-to-diagnose cases of oculoglandular tularemia.

Cited by 35 publications

References 10 publications

Detection of New Francisella-Like Tick Endosymbionts in Hyalomma spp. and Rhipicephalus spp. (Acari: Ixodidae) from Bulgaria

Detection of New Francisella-Like Tick Endosymbionts in Hyalomma spp. and Rhipicephalus spp. (Acari: Ixodidae) from Bulgaria

Tularemia in Bulgaria 2003-2004

Tularemia: current diagnosis and treatment options

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