Diagnostic assays for two closely related tospovirus species, Watermelon bud necrosis virus and Groundnut bud necrosis virus and identification of new natural hosts

Genetic analysis of resistance to watermelon bud necrosis orthotospovirus in watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai]

“…To specifically identify WBNV, RT‐PCR was performed using primers specific to the nucleocapsid (N) gene of WBNV as described by Holkar et al. ().…”

“…DAC-ELISA was performed using general orthotospovirus antiserum as per the protocol described by Hobbs, Reddy, Rajeshwari, and Reddy (1987). To specifically identify WBNV, RT-PCR was performed using primers specific to the nucleocapsid (N) gene of WBNV as described by Holkar et al (2017).…”

“…It was first recorded during 1991 infecting watermelon at Indian Institute of Horticultural Research (IIHR), Bangalore, India (Singh & Krishnareddy, ) and later found to infect several other cucurbits, such as cucumber, ridge gourd and muskmelon (Jain, Bag, Umamaheswaran, & Mandal, ; Jain et al., ; Kumar, Mandal, Geetanjali, Jain, & Jaiwal, ; Mandal, Jain, Chaudhary, & Varma, ). Recently, WBNV was also detected in chilli pepper, tomato (Kunkalikar et al., ) and chrysanthemum (Holkar et al., ) in northern India. WBNV is widely distributed and endemic in many states of India, such as Andhra Pradesh, Gujarat, Haryana, Himachal Pradesh, Karnataka, Kerala, Madhya Pradesh, Maharashtra, Orissa, Punjab, Rajasthan, Tamil Nadu, Uttar Pradesh and West Bengal (Mandal et al., ).…”

“…Recently, WBNV was also detected in chilli pepper, tomato (Kunkalikar et al, 2011) and chrysanthemum (Holkar et al, 2017) in northern India. WBNV is widely distributed and endemic in many states of India, such as Andhra Pradesh, Gujarat, Haryana, Himachal Pradesh, Karnataka, Kerala, Madhya Pradesh, Maharashtra, Orissa, Punjab, Rajasthan, Tamil Nadu, Uttar Pradesh and West Bengal (Mandal et al, 2012).…”

“…CaCV which is a serious pathogen of capsicum and chilli was also reported to infect tomato and groundnut (Chen, Xu, Yan, & Wang, 2007;Persley, Thomas, &Sharman, 2006 andPremachandra, Borgemeister, Maiss, Knierim, &Poehling, 2005), but not yet reported to infect watermelon. GBNV was found not to infect watermelon (Holkar et al, 2017). In India, WBNV is naturally transmitted by a melon thrips, Thrips palmi Karny (Rajasekharam, 2010;Rebijith, Asokan, Hande, & Kumar, 2016;Rebijith, Asokan, Krishna Kumar, Krishna, & Ramamurthy, 2012).…”