Diagnostic challenges in von Willebrand disease. Report of two cases with emphasis on multimeric and molecular analysis

Moreno-Castaño, AB; Ramos, Angela; Pino, Marcos; Parra, Rafael; Altisent, Carme; Vidal, Francisco; Corrales, Irene; Borràs, Nina; Torramadé-Moix, Sergi; Palomo, Marta; Escolar, Ginés; Díaz‐Ricart, Maribel

doi:10.1080/09537104.2020.1784403

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Assessment Of Circulating Biomarkers1

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2022

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“…Visualization of VWF multimers was achieved using a commercially available enhanced chemiluminescence kit for detecting horseradish peroxidaselabeled antibodies on Western blots. 29 In addition, VWF-cleaving protease (ADAMTS13) activity was assessed by fluorescence resonance energy transfer (Fluoroskan Ascent FL, Thermo Fisher Scientific, Waltham, MA). Plasminogen activator inhibitor antigen (PAI) and thrombomodulin (TM) were measured by ELISA (Imubind, BioMedica Diagnostics, Windsor, Canada and Biomatik Corporation, respectively).…”

Section: Assessment Of Circulating Biomarkersmentioning

confidence: 99%