Diagnostic criteria for ductal adenocarcinoma of the prostate: interobserver variability among 20 expert uropathologists

Seipel, Amanda H.; Delahunt, Brett; Samaratunga, Hemamali; Amin, Mahul B.; Barton, Joel; Berney, Daniel M.; Billis, Athanase; Liu, Cheng; Compérat, Eva; Evans, Andrew; Fine, Samson W.; Grignon, David J.; Humphrey, Peter A.; Magi‐Galluzzi, Cristina; Montironi, Rodolfo; Sesterhenn, Isabell A.; Srigley, John R.; Trpkov, Kiril; Kwast, Theodorus H. van der; Varma, Murali; Zhou, Ming; Ahmad, Amar; Moss, Steve; Egevad, Lars

doi:10.1111/his.12382

Cited by 41 publications

(30 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of interest, the only other study to investigate ERG protein expression in ductal adenocarcinomas did not find a lower rate of expression in these tumors versus acinar carcinomas . Though this study did not do confirmatory FISH, this discrepancy with our current results raises the interesting question of whether variable criteria for diagnosing ductal adenocarcinoma (vs acinar carcinoma) may in part explain these differences . Indeed, ductal adenocarcinoma and intraductal spread of acinar carcinoma of the prostate have overlapping morphologic features and previous work by our group has suggested a relatively high rate of ERG expression among the latter entity .…”

Section: Discussioncontrasting

confidence: 99%

PTEN loss and ERG protein expression are infrequent in prostatic ductal adenocarcinomas and concurrent acinar carcinomas

et al. 2015

View full text Add to dashboard Cite

Background Prostatic ductal adenocarcinoma is an unusual and aggressive morphologic subtype of prostate cancer. PTEN gene deletion and ERG gene rearrangement are among the most common genomic changes in acinar prostate cancers. Though ductal adenocarcinoma most commonly occurs with synchronous usual-type acinar adenocarcinoma, little is known about the molecular phenotype of these mixed tumors. Methods We used genetically validated immunohistochemistry (IHC) assays to assess PTEN and ERG status in a group of 37 surgically treated ductal adenocarcinomas and 18 synchronous acinar adenocarcinomas where we have previously reported ERG gene rearrangement status by fluorescence in situ hybridization (FISH). A group of 34 stage and grade-matched pure acinar adenocarcinoma cases was studied as a control. Results ERG IHC was highly concordant with ERG FISH results, with 100% (36/36) concordance among ductal adenocarcinomas and 91% (31/34) concordance among 34 pure acinar carcinomas. Similar to previous FISH results, ERG expression by IHC was significantly less common among ductal adenocarcinomas (11% or 4/37) and their synchronous acinar tumors (6% or 1/18) compared to matched pure acinar adenocarcinoma cases (50% or 17/34; p= 0.0005 and 0.002, respectively). PTEN loss by IHC was also less common among ductal adenocarcinomas (18% or 6/34) and their synchronous acinar tumors (22% or 4/18) compared to matched pure acinar carcinomas (50% or 17/34; p = 0.01 and 0.08, respectively). As expected, PTEN loss was enriched among ERG positive compared to ERG-negative tumors in the pure acinar tumor control group (2.5-fold enrichment; p=0.04) however this was not observed among the ductal adenocarcinomas (1.5 fold enrichment; p=NS). Of ductal adenocarcinomas with an evaluable synchronous acinar component, ERG status was concordant in 94% (17/18) and PTEN status was concordant in 94% (16/17). Conclusions Based on PTEN and ERG, ductal adenocarcinomas and their concurrent acinar carcinomas may be clonally related in some cases and show important molecular differences from pure acinar carcinoma.

show abstract

Section: Discussioncontrasting

confidence: 99%

PTEN loss and ERG protein expression are infrequent in prostatic ductal adenocarcinomas and concurrent acinar carcinomas

et al. 2015

View full text Add to dashboard Cite

show abstract

“…In our series, CDX2 was positive in nine DAC cases and all colon adenocarcinomas. Immunoreactivity for CDX2 in acinar prostatic adenocarcinoma has been reported in 5.7% of cases , which compares to 15% in DAC in the current study; however, a previous study from our group did not show a statistically significant difference in staining between DAC and acinar adenocarcinomas . Herawi et al found that the staining was independent of GS and growth pattern.…”

Section: Discussioncontrasting

confidence: 80%

Immunohistochemistry of ductal adenocarcinoma of the prostate and adenocarcinomas of non‐prostatic origin: a comparative study

Seipel

Samaratunga²,

Delahunt

et al. 2016

APMIS

Self Cite

View full text Add to dashboard Cite

Ductal adenocarcinoma of the prostate (DAC) has morphological similarities to adenocarcinomas of other organs. DAC behaves in an aggressive manner and may present with metastases. These metastases may occur at unusual sites, which itself may cause diagnostic difficulties. It is important for therapeutic decisions that a prostatic origin of these metastases be established. Our aim was to compare the protein expression of DAC and adenocarcinomas of colon, endometrium, lung, pancreas, stomach and urinary bladder. A tissue microarray was constructed using 60 DAC, 6 colonic, 7 endometrial, 7 lung, 5 pancreatic, 5 gastric, and 9 urinary bladder adenocarcinomas. Slides were stained for estrogen, progesterone and androgen receptor, prolactin, PSA, prostein, PSMA, PSAP, CDX2, lysozyme, villin, monoclonal CEA, CK7, CK20, HMWCK, p63, p504s, c‐Myc, EGFR, Ki‐67, p16, p21, p27, p53, PTEN, ERG, and PAX‐8. Androgen receptor, prostein, PSA, and PSAP were almost invariably expressed in DAC. Ki‐67‐labeling index was lower in DAC than in other adenocarcinomas. The expression patterns of intestinal markers and cytokeratins in DAC were less specific and may lead to diagnostic errors if not combined with prostate‐specific markers.

show abstract

“…7,30,36 In a reproducibility study among international experts, papillary architecture was considered the most helpful feature for diagnosing DAC, followed by cellular features consisting of high-grade nuclear atypia, tall columnar epithelium and elongated nuclei. 37 Cribriform patterns and necrosis were considered more non-specific, while the lack of typical architecture was the most common cause for not assigning a DAC diagnosis. There is no evidence that necrosis, percentage of DAC or stromal invasion versus intraductal spread have any clinical significance.…”

Section: Histopathologymentioning

confidence: 99%

“…37 Points of difference are that its cells are less columnar and may form cribriform patterns with rounded lumina or micropapillary tufts without fibrovascular stalks. The nuclei are more rounded and the epithelium not as columnar as those seen in DAC, but the distinction is not always easily made.…”

Section: Differential Diagnosesmentioning

confidence: 99%

Ductal adenocarcinoma of the prostate: histogenesis, biology and clinicopathological features

et al. 2016

Self Cite

View full text Add to dashboard Cite

Diagnostic criteria for ductal adenocarcinoma of the prostate: interobserver variability among 20 expert uropathologists

Abstract: Papillary architecture was the most useful diagnostic feature of DAC, and nuclear and cellular features were considered to be less important.

Cited by 41 publications

References 30 publications

PTEN loss and ERG protein expression are infrequent in prostatic ductal adenocarcinomas and concurrent acinar carcinomas

PTEN loss and ERG protein expression are infrequent in prostatic ductal adenocarcinomas and concurrent acinar carcinomas

Immunohistochemistry of ductal adenocarcinoma of the prostate and adenocarcinomas of non‐prostatic origin: a comparative study

Ductal adenocarcinoma of the prostate: histogenesis, biology and clinicopathological features

Contact Info

Product

Resources

About