Diagnostic Efficacy of Endometrial Cytology with the Abradul Cell Sampler Supplemented by Laser Scanning Confocal Microscopy

Boon, Mathilde E.; Luzzatto, Rui; Brücker, Newton; Recktenvald, Marta; Benita, E. M.

doi:10.1159/000333751

Cited by 6 publications

(2 citation statements)

References 16 publications

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“…When analyzing immunoreactivity localization of cell smears, conventional fluorescence or light microscopy generally cannot provide adequate information on the subcellular distribution. 1,2 In this study, CLSM observations clearly demonstrated different intranuclear localization of the antigen. This instrument has vast potentials not only in analyzing diagnostic cytologic material, including immunohistochemistry, but also in studying the intranuclear arrangement of chromatin.…”

Section: Discussionsupporting

confidence: 59%

“…6 Confocal laser scanning microscopy (CLSM) is an exciting new tool with rapidly expanding potential for morphologic analysis, especially in studying the three-dimensional morphology of biologic objects. 1,2,13 When considering the clinical application of CLSM, cell smears can be of greater value and potential than tissue sections. 11 Therefore, as an initial step in examining the potential of CLSM observations on diagnostic human surgical pathology materials, we used CLSM to analyze p53 immunolocalization in cell smears of human endometrial carcinoma.…”

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confidence: 99%

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Cytologic Application of p53 Overexpression Using Immunocytochemistry and Confocal Laser Scanning Microscopy

et al. 1998

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Section: Discussionsupporting

confidence: 59%

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confidence: 99%

Cytologic Application of p53 Overexpression Using Immunocytochemistry and Confocal Laser Scanning Microscopy

et al. 1998

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Rescreening of atypical cervicovaginal smears using PAPNET

Lerma

Colomo

Carreras

et al. 1998

Cancer

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BACKGROUND Atypical squamous cells of undetermined significance (ASCUS) is a cytopathologic term used to describe cases without specific pathologic substratum. Between 10‐60% of ASCUS cases correspond to squamous intraepithelial lesions (SIL). METHODS The objectives of this study were: 1) to detect the pathologic significance of ASCUS in study patients, 2) to determine whether PAPNET identifies these cases, and 3) to compare the results of PAPNET with those of a second conventional screening. One hundred and sixty‐three consecutive patients with the cytologic diagnosis of ASCUS and adequate follow‐up were selected. Of these, 111 patients had colposcopic lesions and biopsies were performed; in the remaining 52 cases colposcopy was negative, as were 3 consecutive annual Papanicolaou smears. In a blind review, all 163 cases were rescreened using PAPNET. A second manual screening was performed for comparison. RESULTS One hundred and twenty‐six of the 163 cases (77.3%) showed no SIL on biopsy or follow‐up. Of the 37 pathologic cases, the diagnosis was koilocytosis (flat condyloma) in 13 cases (8%), cervical intraepithelial neoplasia (CIN) type I in 11 cases (6.8%) low grade SIL [LSIL] in a total of 24 cases [14.8%]), and CIN II‐III or high grade SIL (HSIL) in 11 cases (6.8%). In the review using PAPNET, 57 previous ASCUS cases were classified correctly as negative, and 7 of 13 koilocytosis cases (54%), 9 of 11 CIN I cases (82%), and 7 of 11 CIN II‐III cases (64%) were diagnosed correctly. In the second conventional screening, 74 cases were negative and 77 cases were ASCUS; only 3 of 13 koilocytosis cases (23%), 4 of 11 CIN I cases (36.4%) and 5 of 11 CIN II‐III cases (45.5%) were reclassified correctly. CONCLUSIONS Among 163 patients with ASCUS, 77.3% had no precancerous squamous lesions. Concordance with definitive diagnosis was more accurate in our study using PAPNET analysis (Kappa index [K] = 0.7158) than by second conventional screening (K = 0.4537). Furthermore, we reclassified 35% of smears as negative and 15% as SIL. Cancer (Cancer Cytopathol) 1998;84:361‐365. © 1998 American Cancer Society.

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A primer of endometrial cytology with histological correlation

Maksem

Meiers

Robboy

2007

Diagnostic Cytopathology

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Cytology is an effective method for assessing benign endometrium and for discovering premalignant and malignant endometrial states. In addition, it is useful for diagnosing non-neoplastic abnormalities of the endometrium. This overview compares endometrial cytology to endometrial histology for a variety of benign, abnormal non-neoplastic, and neoplastic conditions; and, discusses both diagnostic criteria and pitfalls in the assessment of endometrial brushings specimens. It also makes an attempt to estimate levels of confidence in endometrial cytodiagnosis. When endometrial brushing is used in conjunction with other diagnostic techniques such as ultrasonography/sonohysterography or hysteroscopy, cytology becomes a sensitive case finding technique that shows good patient acceptance (because of a significant decrease in procedure-associated pain) and high diagnostic yield.

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Diagnostic Efficacy of Endometrial Cytology with the Abradul Cell Sampler Supplemented by Laser Scanning Confocal Microscopy

Cited by 6 publications

References 16 publications

Cytologic Application of p53 Overexpression Using Immunocytochemistry and Confocal Laser Scanning Microscopy

Cytologic Application of p53 Overexpression Using Immunocytochemistry and Confocal Laser Scanning Microscopy

Rescreening of atypical cervicovaginal smears using PAPNET

A primer of endometrial cytology with histological correlation

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